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[186469_at]

Description C28C12.8 /REP_DB=WormBase Gene ID /WP=CE04115 /TR=Q18277 /GB=AAB37551.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 73      4grp_total > 09      6grp_cgc > 76      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 246
1 2 3 4 range
A 86.8
(-3)
M 		108.5
(-2.3)
A 		121.7
(-1.9)
A 		273.9
(3)
P 		187
B 76.4
(-3)
A 		145
(-0.6)
P 		161.7
(-0.1)
P 		251.3
(3)
P 		175
C 147.2
(-1.9)
A 		110.1
(-3)
A 		109.1
(-3)
P 		321.9
(3)
P 		213
avr 103.5
(-3) 		121.2
(-2.4) 		130.8
(-2.1) 		282.4
(3) 		179

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 131
1 2 3 4 5 6 range
A 49.6
(-3)
A 		64.9
(-1.9)
A 		68.6
(-1.6)
A 		132.7
(3)
P 		111.3
(1.5)
M 		82.8
(-0.6)
P 		83
B 25.1
(-3)
A 		44.7
(-2.1)
A 		104.7
(0.6)
P 		156.1
(3)
P 		87.2
(-0.2)
P 		59.3
(-1.4)
P 		131
C 64.3
(-2.7)
A 		92.3
(0.1)
A 		120.6
(3)
P 		117.8
(2.7)
A 		61.7
(-3)
A 		87.5
(-0.4)
A 		59
avr 46.3
(-3) 		67.3
(-1.6) 		98
(0.5) 		135.5
(3) 		86.7
(-0.3) 		76.5
(-1) 		89
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 31.6
(-0.1)
M 		23.5
(-3)
A 		31.8
(0)
A 		40.1
(3)
P 		17
B 24.4
(-3)
A 		35.8
(-0.7)
P 		37.5
(-0.3)
P 		53.6
(3)
P 		29
C 48.7
(1.5)
A 		31.6
(-3)
A 		38
(-1.3)
P 		54.3
(3)
P 		23
avr 34.9
(-1.5) 		30.3
(-3) 		35.8
(-1.3) 		49.3
(3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 9.1
(-3)
A 		23.1
(0.4)
A 		14.9
(-1.6)
A 		33.7
(3)
P 		33.6
(3)
M 		20.2
(-0.3)
P 		25
B 8.7
(-3)
A 		14.9
(-1.9)
A 		25.4
(0.1)
P 		41.3
(3)
P 		35.4
(1.9)
P 		22.1
(-0.5)
P 		33
C 23.8
(-0.9)
A 		28.1
(1.5)
A 		30.7
(3)
P 		23.7
(-0.9)
A 		20
(-3)
A 		29.3
(2.2)
A 		11
avr 13.9
(-3) 		22
(-0.4) 		23.7
(0.1) 		32.9
(3) 		29.7
(2) 		23.9
(0.2) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186469_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10074
Sequence Type Exemplar sequence
Representative Public ID C28C12.8 NCBI
Target Description C28C12.8 /REP_DB=WormBase Gene ID /WP=CE04115 /TR=Q18277 /GB=AAB37551.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069044(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069044 NCBI Caenorhabditis elegans Helix Loop Helix family member (hlh-12) (hlh-12) mRNA, complete cds. 11/11 None
SNAP00000012162 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8476793:8479428:-1 11/11 None
GENEFINDER00000012175 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8478622:8479428:-1 11/11 None
C28C12.8 ENSEMBL cdna:known chromosome:CEL140:IV:8478622:8479428:-1 gene:C28C12.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8478627-8479434(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol hlh-12
Ensembl C28C12.8
Entrez Gene 182980 Entrez gene
SwissProt Q18277 EMBL-EBI
WormBase CE04115 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37551 Helix loop helix protein 12 [Caenorhabditis elegans] ref|NP_501445.1| Helix Loop Helix family member (hlh-12) [Caenorhabditis elegans] 2.0E-73
blast AAB37551 Helix loop helix protein 12 [Caenorhabditis elegans] ref|NP_501445.1| Helix Loop Helix family member (hlh-12) [Caenorhabditis elegans] 2.0E-69
blast CAE56699 Hypothetical protein CBG24484 [Caenorhabditis briggsae] 3.0E-19
blast CAE56699 Hypothetical protein CBG24484 [Caenorhabditis briggsae] 8.0E-19
blast CAE71553 Hypothetical protein CBG18500 [Caenorhabditis briggsae] 9.0E-18
Protein Domains
Database ID Description E-Value
Pfam IPR001092 EMBL-EBI Basic helix-loop-helix dimerization domain bHLH 1.4E-16
Sequence
Target Sequence 
>C. ELEGANS:186469_AT
aaccgagagttaccaagctgaatacggatcgaagatcgagagcaaacgaacgagaacgac
agagagtttccgagatgaacggaatgtttgatgttctacttaatcttcttccgccgtcac
attttaaaacacgactctccagagttcaaatcttgagagaagccacctcttacataattc
gtcttcacaactttcttattgaaagttcaaatagtgacatagacgcaataactgtatttc
cgcacatcttcaacggagaacgcaagtcaaataaagatgcaattcgtcgtcccatgaagt
tgaagcagggtggcggtgttgctgctttcatctcacgccacgaacttcccccacttcaac
ttccaaatcccgttatcccaattttgaagcccacttcagttccagttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACCGAGAGTTACCAAGCTGAATAC 707 143 23 Antisense
GATGTTCTACTTAATCTTCTTCCGC 82 411 112 Antisense
TCTTCCGCCGTCACATTTTAAAACA 665 617 129 Antisense
ACATTTTAAAACACGACTCTCCAGA 67 103 141 Antisense
GACTCTCCAGAGTTCAAATCTTGAG 183 375 155 Antisense
GAAGCCACCTCTTACATAATTCGTC 394 345 181 Antisense
ATAACTGTATTTCCGCACATCTTCA 477 25 250 Antisense
CGCACATCTTCAACGGAGAACGCAA 111 255 263 Antisense
GCAATTCGTCGTCCCATGAAGTTGA 221 323 301 Antisense
AGCAGGGTGGCGGTGTTGCTGCTTT 31 79 326 Antisense
TTGAAGCCCACTTCAGTTCCAGTTT 353 705 406 Antisense
Sequence Source Affymetrix Proprietary Database