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[186456_at]

Description T12B5.12 /REP_DB=WormBase Gene ID /WP=CE20069 /TR=Q9TZ20 /GB=AAC68935.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 70      4grp_total > 20      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 28
(-0)
A 		22.4
(-1.9)
A 		19.3
(-3)
A 		36.9
(3)
A 		18
B 32.3
(0.6)
A 		33.8
(0.9)
A 		10.6
(-3)
A 		46.6
(3)
P 		36
C 32.9
(-0.8)
A 		27.5
(-1.4)
A 		14
(-3)
A 		64.4
(3)
A 		50
avr 31.1
(-0.1) 		27.9
(-0.7) 		14.6
(-3) 		49.3
(3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 42
(0.7)
A 		28.7
(-3)
P 		50.2
(3)
A 		39.9
(0.1)
P 		46.1
(1.9)
A 		35.4
(-1.1)
A 		22
B 25
(-3)
A 		27.7
(-2.6)
A 		61.8
(2.3)
P 		66.3
(3)
P 		38.1
(-1.1)
P 		29.6
(-2.3)
A 		41
C 32.5
(0.3)
A 		48.4
(2.2)
P 		4.8
(-3)
A 		55.1
(3)
A 		20.7
(-1.1)
A 		33.7
(0.4)
P 		50
avr 33.2
(-2.9) 		34.9
(-2.4) 		38.9
(-1.3) 		53.8
(3) 		35
(-2.4) 		32.9
(-3) 		21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 10.2
(3)
A 		4.9
(-3)
A 		5.1
(-2.8)
A 		5.4
(-2.4)
A 		5
B 10.3
(3)
A 		8.3
(1.5)
A 		2.5
(-3)
A 		9.9
(2.7)
P 		8
C 10.9
(3)
A 		7.9
(0)
A 		4.9
(-3)
A 		10.9
(3)
A 		6
avr 10.5
(3) 		7
(-0.3) 		4.2
(-3) 		8.7
(1.3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 7.7
(-3)
A 		10.2
(-0.6)
P 		10.9
(0.1)
A 		10.1
(-0.7)
P 		13.9
(3)
A 		8.6
(-2.1)
A 		6
B 8.7
(-3)
A 		9.2
(-2.7)
A 		15
(1.3)
P 		17.5
(3)
P 		15.5
(1.6)
P 		11
(-1.4)
A 		9
C 12.1
(1.8)
A 		14.8
(3)
P 		1.2
(-3)
A 		11.1
(1.4)
A 		6.7
(-0.6)
A 		11.3
(1.5)
P 		14
avr 9.5
(-2.2) 		11.4
(0.7) 		9
(-3) 		12.9
(3) 		12
(1.6) 		10.3
(-1) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186456_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8150
Sequence Type Exemplar sequence
Representative Public ID T12B5.12 NCBI
Target Description T12B5.12 /REP_DB=WormBase Gene ID /WP=CE20069 /TR=Q9TZ20 /GB=AAC68935.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064905(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064905 NCBI Caenorhabditis elegans T12B5.12 (T12B5.12) mRNA, complete cds. 10/11 None
T12B5.12 ENSEMBL cdna:known chromosome:CEL140:III:960285:961334:-1 gene:T12B5.12 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:960254-961334(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T12B5.12
Entrez Gene 188449 Entrez gene
SwissProt Q9TZ20 EMBL-EBI
WormBase CE32933 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68935 Hypothetical protein T12B5.12 [Caenorhabditis elegans] ref|NP_497306.2| T12B5.12 [Caenorhabditis elegans] 9.0E-64
blast AAK29719 Hypothetical protein Y119D3B.7 [Caenorhabditis elegans] ref|NP_497354.1| Y119D3B.7 [Caenorhabditis elegans] 1.0E-15
blast AAC04395 Hypothetical protein C17B7.11 [Caenorhabditis elegans] ref|NP_503929.1| C17B7.11 [Caenorhabditis elegans] 5.0E-15
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 7.4E-11
Sequence
Target Sequence 
>C. ELEGANS:186456_AT
gaacctaatggtggtaccactcacgcatcaatatttttcattaaaaacaatttagaaaat
agacacgacattatcacatcttttctggaaactttgaaatcagttggatgtattcacgcg
aaaaaaacgataattgaaaagctctcattcgaacatgtcatttctattcttccatatttt
gatgccaaaacgctgaaaaatattcttttgatagaaacggaccaagttgttcaattcgaa
cggatcattcctttggatcagtggaaaaatgcaacaaagtatggatattgttgttatcct
tccagcactaaacaacttgaacattgctttcactttgaagaatttactattgaaattgat
gaattatcagtacagaatgcagttcaaattagagatgatttaatgaaaaggagtacattt
caaaaatgcgaaataagttttgccaaatcggcaaaccttattaaaattgcgaaagtattc
aaatcagactatgctggtgacatctattttaagtttgaatattcaaacaataatgacact
tttgccattgaatgtcagtctcatctttggttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACCTAATGGTGGTACCACTCACG 428 345 292 Antisense
ACCACTCACGCATCAATATTTTTCA 608 85 307 Antisense
GAACATGTCATTTCTATTCTTCCAT 464 351 442 Antisense
ATTCTTCCATATTTTGATGCCAAAA 548 11 457 Antisense
ATTCGAACGGATCATTCCTTTGGAT 552 7 525 Antisense
GATATTGTTGTTATCCTTCCAGCAC 20 427 575 Antisense
ATCCTTCCAGCACTAAACAACTTGA 222 39 587 Antisense
CTTGAACATTGCTTTCACTTTGAAG 710 191 607 Antisense
ATCAGACTATGCTGGTGACATCTAT 198 29 774 Antisense
GACACTTTTGCCATTGAATGTCAGT 246 367 826 Antisense
TGAATGTCAGTCTCATCTTTGGTTT 504 575 840 Antisense
Sequence Source Affymetrix Proprietary Database