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[186454_at]

Description T25F10.1 /REP_DB=WormBase Gene ID /WP=CE07532 /TR=Q23051 /GB=AAB04990.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 29      4grp_total > 35      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 92
1 2 3 4 range
A 36.1
(3)
A 		19.2
(-1.3)
A 		22.2
(-0.6)
A 		12.7
(-3)
A 		23
B 13.8
(-2.8)
A 		101.8
(3)
A 		10.5
(-3)
A 		61.4
(0.3)
A 		91
C 10.6
(-2.8)
A 		22.7
(3)
A 		10.1
(-3)
A 		20
(1.7)
A 		13
avr 20.2
(-1.9) 		47.9
(3) 		14.3
(-3) 		31.4
(0.1) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 67
1 2 3 4 5 6 range
A 73.5
(3)
A 		16.2
(-2.4)
A 		9.6
(-3)
A 		9.8
(-3)
A 		25.4
(-1.5)
A 		21.7
(-1.9)
A 		64
B 15.6
(-1.6)
A 		6.1
(-3)
A 		9.9
(-2.4)
A 		16.1
(-1.5)
A 		47.1
(3)
A 		7.1
(-2.9)
A 		41
C 12.9
(-2.8)
A 		59.3
(3)
A 		57.3
(2.8)
A 		15.9
(-2.4)
A 		25
(-1.3)
A 		11.2
(-3)
A 		48
avr 34
(3) 		27.2
(1) 		25.6
(0.6) 		13.9
(-2.8) 		32.5
(2.6) 		13.3
(-3) 		21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 13.1
(3)
A 		4.2
(-1.8)
A 		5.8
(-0.9)
A 		1.9
(-3)
A 		11
B 4.4
(-2.5)
A 		25.1
(3)
A 		2.4
(-3)
A 		13.1
(-0.2)
A 		23
C 3.5
(-2.8)
A 		6.5
(3)
A 		3.5
(-2.8)
A 		3.4
(-3)
A 		3
avr 7
(-0.7) 		11.9
(3) 		3.9
(-3) 		6.1
(-1.4) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 13.5
(3)
A 		5.8
(-1.1)
A 		2.1
(-3)
A 		2.5
(-2.8)
A 		7.7
(-0.1)
A 		5.3
(-1.3)
A 		11
B 5.4
(-1.8)
A 		2
(-3)
A 		2.4
(-2.9)
A 		4.3
(-2.2)
A 		19.2
(3)
A 		2.6
(-2.8)
A 		17
C 4.8
(-2.4)
A 		18.1
(3)
A 		14.6
(1.6)
A 		3.2
(-3)
A 		8.1
(-1)
A 		3.7
(-2.8)
A 		15
avr 7.9
(0.3) 		8.6
(0.8) 		6.4
(-0.8) 		3.3
(-3) 		11.7
(3) 		3.9
(-2.6) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186454_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12068
Sequence Type Exemplar sequence
Representative Public ID T25F10.1 NCBI
Target Description T25F10.1 /REP_DB=WormBase Gene ID /WP=CE07532 /TR=Q23051 /GB=AAB04990.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072312(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072312 NCBI Caenorhabditis elegans T25F10.1 (T25F10.1) mRNA, complete cds. 11/11 None
SNAP00000017682 ENSEMBL cdna:SNAP chromosome:CEL140:V:6771096:6771380:1 11/11 None
GENEFINDER00000017687 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6771096:6772075:1 11/11 None
T25F10.1 ENSEMBL cdna:known chromosome:CEL140:V:6771096:6771380:1 gene:T25F10.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001028988 11/11 Negative Strand Matching Probes None
T25F10.6b.1 11/11 Negative Strand Matching Probes A 
T25F10.6b.2 11/11 Negative Strand Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6771096-6771381(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T25F10.1
Entrez Gene 188902 Entrez gene
SwissProt Q23051 EMBL-EBI
WormBase CE07532 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB04990 Hypothetical protein T25F10.1 [Caenorhabditis elegans] ref|NP_504713.1| T25F10.1 [Caenorhabditis elegans] 2.0E-48
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000017687 1 98-120
Sequence
Target Sequence 
>C. ELEGANS:186454_AT
tgccttcgacgatcgctcaaagtaggtgggcggagtctgccacatccttcccgacggcaa
cggtggcggcggcgggaggaaaagatatcgaaatcgagagagacgtcgcccacaatcaac
caggaggcatcttcccgaatgtaatattcttggaatctcttgacttctcggcgtgtttcg
tcgatgctcaccaagtcttcgtctccgacggaagtgtaatgtttcgacgatggga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCCTTCGACGATCGCTCAAAGTAG 582 585 32 Antisense
CGACGATCGCTCAAAGTAGGTGGGC 482 243 38 Antisense
GTAGGTGGGCGGAGTCTGCCACATC 65 457 53 Antisense
TGGCGGCGGCGGGAGGAAAAGATAT 24 541 95 Antisense
GAAATCGAGAGAGACGTCGCCCACA 105 363 121 Antisense
GTCGCCCACAATCAACCAGGAGGCA 586 473 136 Antisense
AATCAACCAGGAGGCATCTTCCCGA 513 171 145 Antisense
GGAGGCATCTTCCCGAATGTAATAT 573 515 154 Antisense
TCTTGGAATCTCTTGACTTCTCGGC 303 619 179 Antisense
CCAAGTCTTCGTCTCCGACGGAAGT 550 267 222 Antisense
GAAGTGTAATGTTTCGACGATGGGA 279 335 242 Antisense
Sequence Source Affymetrix Proprietary Database