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[186449_at]

Description Y46G5A.19 /REP_DB=WormBase Gene ID /WP=CE21997 /TR=Q9U2F0 /GB=CAB60361.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 22      4grp_highest_8_8 > 24      4grp_high_8_8 > 52      6grp_highest_nonorm > 80      6grp_total > 21      4grp_total > 60      6grp_highest_sub > 02      6grp_high1_sub > De      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 205
1 2 3 4 range
A 326.9
(3)
P 		313.8
(2.6)
P 		288
(1.7)
P 		146.2
(-3)
P 		181
B 328.4
(1.8)
P 		333
(2.1)
P 		350.8
(3)
P 		237
(-3)
P 		114
C 240.4
(0.3)
P 		294.2
(2.5)
P 		307
(3)
P 		159.4
(-3)
P 		148
avr 298.6
(2.3) 		313.7
(2.9) 		315.3
(3) 		180.9
(-3) 		134

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 178
1 2 3 4 5 6 range
A 295.8
(3)
P 		256.4
(1.5)
P 		244.4
(1)
P 		205.5
(-0.5)
P 		142.6
(-3)
P 		161.6
(-2.3)
P 		153
B 164.2
(-1.6)
P 		300.9
(3)
P 		201.9
(-0.3)
P 		213.5
(0.1)
P 		122.7
(-3)
P 		151.7
(-2)
P 		178
C 202.7
(-0.6)
P 		254.2
(2.3)
P 		266.5
(3)
P 		197
(-0.9)
P 		183.7
(-1.7)
P 		160
(-3)
P 		107
avr 220.9
(0.5) 		270.5
(3) 		237.6
(1.4) 		205.3
(-0.2) 		149.7
(-3) 		157.8
(-2.6) 		121
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 119
(3)
P 		68.1
(-0.1)
P 		75.3
(0.3)
P 		21.4
(-3)
P 		98
B 104.7
(3)
P 		82.2
(0.5)
P 		81.3
(0.4)
P 		50.5
(-3)
P 		54
C 79.6
(0.9)
P 		84.6
(1.3)
P 		107
(3)
P 		26.9
(-3)
P 		80
avr 101.1
(3) 		78.3
(1) 		87.9
(1.8) 		32.9
(-3) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 61
1 2 3 4 5 6 range
A 54.5
(-1.2)
P 		91.2
(3)
P 		53.2
(-1.4)
P 		52.1
(-1.5)
P 		43
(-2.6)
P 		39.3
(-3)
P 		52
B 57.1
(-2.1)
P 		100.2
(3)
P 		49
(-3)
P 		56.6
(-2.1)
P 		49.9
(-2.9)
P 		56.6
(-2.1)
P 		51
C 75.1
(2.6)
P 		77.6
(3)
P 		67.8
(1.5)
P 		39.6
(-3)
P 		59.4
(0.1)
P 		53.6
(-0.8)
P 		38
avr 62.2
(-1.1) 		89.7
(3) 		56.7
(-1.9) 		49.4
(-3) 		50.8
(-2.8) 		49.8
(-2.9) 		40
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186449_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2923
Sequence Type Exemplar sequence
Representative Public ID Y46G5A.19 NCBI
Target Description Y46G5A.19 /REP_DB=WormBase Gene ID /WP=CE21997 /TR=Q9U2F0 /GB=CAB60361.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064322, AJ306734
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000038999 ENSEMBL cdna:SNAP chromosome:CEL140:II:12806805:12810693:1 11/11 None
GENEFINDER00000039031 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:12806805:12810693:1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_064322 5/11 Cross Hyb Matching Probes None
AJ306734 5/11 Cross Hyb Matching Probes None
Y46G5A.19.2 5/11 Cross Hyb Matching Probes None
Y46G5A.19.1 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:12806801-12810690(+) 100.0 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE63440 Hypothetical protein CBG07888 [Caenorhabditis briggsae] 1.0E-180
blast CAB60361 Hypothetical protein Y46G5A.19 [Caenorhabditis elegans] emb|CAC37332.1| spermidine synthase [Caenorhabditis elegans] ref|NP_496723.2| Y46G5A.19 [Caenorhabditis elegans] 1.0E-152
blast NP_033298 spermidine synthase [Mus musculus] emb|CAA91561.1| spermidine synthase [Mus musculus] gb|AAH05566.1| Spermidine synthase [Mus musculus] dbj|BAC34526.1| unnamed protein product [Mus musculus] sp|Q64674|SPEE_MOUSE Spermidine synthase (Putrescine aminopropyltransferase) (SPDSY) gb|AAC37666.1| spermidine synthase prf||2113276A spermidine synthase 2.0E-86
Protein Domains
Database ID Description E-Value
Pfam IPR001045 EMBL-EBI Spermine synthase 1.0E-126
Pfam IPR001045 EMBL-EBI Spermine synthase 8.9E-11
Sequence
Target Sequence 
>C. ELEGANS:186449_AT
acacccgaaactcgacttattctgcggagatggcttcgaatttttgaaaaatcacaagaa
cgagttcgacgtaattatcacggacagttccgatccagttgggccggctgaatcgctttt
cgggcaaagttattacgagttgctgagagatgcgttgaaggaggatggaattttgtcatc
gcaaggcgaatgcccatggctagacatgaagctcatctctaccgtaatccattcggctcg
tgacctatttcccgtggcaaagtacgcggttggcagcgtgccaacctacacgtcaggcct
gatgggctatattttatgcgcaaaggataaggtgaacactggagaatctgtgtggcttca
cctcccactaatcgctcatctcgtcgccttcaatcgaaaaattttcccggccgtcaccta
cgcccagtcgatcgtctctacctacccgtctggaagcatggg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACACCCGAAACTCGACTTATTCTGC 72 109 492 Antisense
CCGGCTGAATCGCTTTTCGGGCAAA 487 263 595 Antisense
GCAAGGCGAATGCCCATGGCTAGAC 208 321 672 Antisense
ACATGAAGCTCATCTCTACCGTAAT 41 107 695 Antisense
TACCGTAATCCATTCGGCTCGTGAC 416 649 711 Antisense
GACCTATTTCCCGTGGCAAAGTACG 85 379 733 Antisense
AAAGTACGCGGTTGGCAGCGTGCCA 549 121 750 Antisense
ACCTACACGTCAGGCCTGATGGGCT 434 87 775 Antisense
GGAGAATCTGTGTGGCTTCACCTCC 189 519 832 Antisense
TCATCTCGTCGCCTTCAATCGAAAA 10 621 867 Antisense
CTACCTACCCGTCTGGAAGCATGGG 429 209 929 Antisense
Sequence Source Affymetrix Proprietary Database