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[186440_at]

Description Y46G5A.14 /REP_DB=WormBase Gene ID /WP=CE21989 /TR=Q9U2F4 /GB=CAB60357.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 78      4grp_total > 01      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 103
1 2 3 4 range
A 50.5
(-0.1)
A 		25.3
(-3)
A 		77.5
(3)
A 		48.5
(-0.3)
A 		52
B 43.6
(0.4)
A 		8.1
(-3)
A 		8.7
(-2.9)
A 		70.7
(3)
A 		63
C 5.7
(-3)
A 		42.6
(-0.9)
A 		7.5
(-2.9)
A 		109
(3)
A 		103
avr 33.3
(-2.1) 		25.3
(-3) 		31.2
(-2.3) 		76.1
(3) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 96
1 2 3 4 5 6 range
A 7
(-2.7)
A 		1.6
(-3)
A 		65.5
(1)
A 		5
(-2.8)
A 		65.2
(1)
A 		97.7
(3)
A 		96
B 48.9
(2.8)
A 		35
(0.8)
A 		20.9
(-1.3)
A 		8.9
(-3)
A 		40.5
(1.6)
A 		50.4
(3)
A 		42
C 8.5
(-3)
A 		31.7
(-0.6)
A 		43
(0.6)
A 		10.1
(-2.8)
A 		12.8
(-2.5)
A 		65.5
(3)
A 		57
avr 21.5
(-1.7) 		22.8
(-1.6) 		43.1
(0.3) 		8
(-3) 		39.5
(-0) 		71.2
(3) 		63
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 18.4
(2.2)
A 		5.5
(-3)
A 		20.3
(3)
A 		7.1
(-2.4)
A 		15
B 13.9
(2.5)
A 		2
(-3)
A 		2
(-3)
A 		15.1
(3)
A 		13
C 1.9
(-3)
A 		12.3
(0.8)
A 		2.6
(-2.7)
A 		18.4
(3)
A 		17
avr 11.4
(1.2) 		6.6
(-3) 		8.3
(-1.5) 		13.5
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 1.3
(-2.8)
A 		0.6
(-3)
A 		14.3
(0.5)
A 		1.3
(-2.8)
A 		19.7
(1.9)
A 		23.8
(3)
A 		23
B 17
(2.3)
A 		11.7
(0.4)
A 		5.1
(-2)
A 		2.4
(-3)
A 		16.4
(2.1)
A 		18.8
(3)
A 		16
C 3.1
(-2.7)
A 		9.7
(-0.7)
A 		11
(-0.3)
A 		2
(-3)
A 		4.1
(-2.4)
A 		21.9
(3)
A 		20
avr 7.1
(-1.4) 		7.3
(-1.3) 		10.1
(-0.5) 		1.9
(-3) 		13.4
(0.5) 		21.5
(3) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186440_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3523
Sequence Type Exemplar sequence
Representative Public ID Y46G5A.14 NCBI
Target Description Y46G5A.14 /REP_DB=WormBase Gene ID /WP=CE21989 /TR=Q9U2F4 /GB=CAB60357.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064318(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064318 NCBI Caenorhabditis elegans Y46G5A.14 (Y46G5A.14) mRNA, complete cds. 11/11 None
SNAP00000038994 ENSEMBL cdna:SNAP chromosome:CEL140:II:12774945:12775398:1 11/11 None
GENEFINDER00000039041 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:12774945:12775398:1 11/11 None
Y46G5A.14 ENSEMBL cdna:known chromosome:CEL140:II:12774622:12775426:1 gene:Y46G5A.14 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027270 1/11 Cross Hyb Matching Probes None
SNAP00000038996 1/11 Cross Hyb Matching Probes None
GENEFINDER00000039028 1/11 Cross Hyb Matching Probes None
Y46G5A.34 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:12774941-12775395(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y46G5A.14
Entrez Gene 174910 Entrez gene
SwissProt Q9U2F4 EMBL-EBI
WormBase CE21989 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB60357 Hypothetical protein Y46G5A.14 [Caenorhabditis elegans] ref|NP_496719.1| Y46G5A.14 [Caenorhabditis elegans] 2.0E-41
blast CAE18011 Hypothetical protein Y46G5A.34 [Caenorhabditis elegans] ref|NP_001022441.1| Y46G5A.34 [Caenorhabditis elegans] 8.0E-29
blast AAM97975 Hypothetical protein Y4C6A.4 [Caenorhabditis elegans] ref|NP_741402.1| Y4C6A.4 [Caenorhabditis elegans] 2.0E-24
Sequence
Target Sequence 
>C. ELEGANS:186440_AT
tattgaactcctcatatccatcaccgtgctcgcgtcgattctcgtctcgtgcaagaaaga
gaagaacgaagcggcaaaactgaagccacgtggcttcgatcgaaaatccagtggagcacc
ggctccggctggagccaacttggttaacaagtctggcggttccggcggaggtagtggagg
atctggagatgctaatagacttctcaaaagagaaatcaaagaagtaaagatggaggagcg
ttcggctgacgataacgagacaatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATTGAACTCCTCATATCCATCACC 641 665 18 Antisense
TCGATTCTCGTCTCGTGCAAGAAAG 257 603 52 Antisense
GCGGCAAAACTGAAGCCACGTGGCT 655 291 88 Antisense
GCCACGTGGCTTCGATCGAAAATCC 109 279 102 Antisense
GAAAATCCAGTGGAGCACCGGCTCC 660 353 119 Antisense
CCGGCTCCGGCTGGAGCCAACTTGG 108 267 136 Antisense
TGGTTAACAAGTCTGGCGGTTCCGG 547 547 158 Antisense
TGGCGGTTCCGGCGGAGGTAGTGGA 109 471 171 Antisense
GAGGTAGTGGAGGATCTGGAGATGC 168 401 185 Antisense
GATGGAGGAGCGTTCGGCTGACGAT 443 407 246 Antisense
TCGGCTGACGATAACGAGACAATCA 375 605 259 Antisense
Sequence Source Affymetrix Proprietary Database