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[186421_at]

Description F21C10.3 /REP_DB=WormBase Gene ID /WP=CE07118 /TR=Q19667 /GB=AAA97977.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 11      6grp_high1_10_10 > 06      4grp_highest_8_8 > 46      4grp_high_8_8 > 10      6grp_highest_nonorm > 41      6grp_total > 05      4grp_total > 14      6grp_high1_sub > De      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1163
1 2 3 4 range
A 1079.2
(0.5)
P 		486.8
(-2.8)
P 		454.2
(-3)
P 		1532.3
(3)
P 		1078
B 1130
(2.4)
P 		615.7
(-1.3)
P 		369.5
(-3)
P 		1218.5
(3)
P 		849
C 1194.5
(2.8)
P 		560.7
(-1.9)
P 		404.8
(-3)
P 		1218.9
(3)
P 		814
avr 1134.6
(1.8) 		554.4
(-2) 		409.5
(-3) 		1323.2
(3) 		914

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 918
1 2 3 4 5 6 range
A 1265.3
(3)
P 		806
(-0)
P 		752.9
(-0.4)
P 		576.2
(-1.5)
P 		561.4
(-1.6)
P 		353.4
(-3)
P 		912
B 1212.1
(3)
P 		818.7
(0.3)
P 		730.9
(-0.3)
P 		719.1
(-0.4)
P 		499.8
(-1.9)
P 		347
(-3)
P 		865
C 1007
(3)
P 		741.6
(0.4)
P 		636
(-0.6)
P 		693.7
(-0.1)
P 		396.9
(-3)
P 		425.5
(-2.7)
P 		610
avr 1161.5
(3) 		788.8
(0.2) 		706.6
(-0.5) 		663
(-0.8) 		486
(-2.2) 		375.3
(-3) 		786
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 310
1 2 3 4 range
A 392.9
(3)
P 		105.7
(-3)
P 		118.7
(-2.7)
P 		224.3
(-0.5)
P 		287
B 360.3
(3)
P 		152.1
(-1.5)
P 		85.6
(-3)
P 		259.7
(0.8)
P 		275
C 395.4
(3)
P 		161.2
(-2.5)
P 		141.1
(-3)
P 		205.5
(-1.5)
P 		254
avr 382.9
(3) 		139.7
(-2.4) 		115.1
(-3) 		229.8
(-0.4) 		268

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 336
1 2 3 4 5 6 range
A 233.3
(1.4)
P 		286.6
(3)
P 		164
(-0.7)
P 		146.2
(-1.2)
P 		169.4
(-0.5)
P 		86
(-3)
P 		201
B 421.8
(3)
P 		272.5
(-0.1)
P 		177.5
(-2)
P 		190.5
(-1.7)
P 		203.1
(-1.5)
P 		129.4
(-3)
P 		292
C 373.3
(3)
P 		226.3
(-0.6)
P 		161.9
(-2.2)
P 		139.5
(-2.7)
P 		128.4
(-3)
P 		142.4
(-2.7)
P 		245
avr 342.8
(3) 		261.8
(0.8) 		167.8
(-1.7) 		158.7
(-1.9) 		167
(-1.7) 		119.3
(-3) 		224
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186421_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12397
Sequence Type Exemplar sequence
Representative Public ID F21C10.3 NCBI
Target Description F21C10.3 /REP_DB=WormBase Gene ID /WP=CE07118 /TR=Q19667 /GB=AAA97977.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171498(11), NM_171499(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171498 NCBI Caenorhabditis elegans F21C10.3a (F21C10.3) mRNA, complete cds. 11/11 None
NM_171499 NCBI Caenorhabditis elegans F21C10.3b (F21C10.3) mRNA, complete cds. 11/11 None
SNAP00000042382 ENSEMBL cdna:SNAP chromosome:CEL140:V:9131517:9133102:1 11/11 None
GENEFINDER00000042384 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9131517:9133102:1 11/11 None
F21C10.3a ENSEMBL cdna:known chromosome:CEL140:V:9131451:9133529:1 gene:F21C10.3 11/11 None
F21C10.3b ENSEMBL cdna:known chromosome:CEL140:V:9131517:9133207:1 gene:F21C10.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9131517-9133103(+) 93.89 100.0
Public Domain and Genome References
Ensembl F21C10.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA97977 Hypothetical protein F21C10.3a [Caenorhabditis elegans] ref|NP_741583.1| F21C10.3a [Caenorhabditis elegans] 1.0E-120
blast AAM29677 Hypothetical protein F21C10.3b [Caenorhabditis elegans] ref|NP_741584.1| F21C10.3b [Caenorhabditis elegans] 1.0E-113
blast AAM29677 Hypothetical protein F21C10.3b [Caenorhabditis elegans] ref|NP_741584.1| F21C10.3b [Caenorhabditis elegans] 1.0E-110
blast AAA97977 Hypothetical protein F21C10.3a [Caenorhabditis elegans] ref|NP_741583.1| F21C10.3a [Caenorhabditis elegans] 1.0E-110
Protein Domains
Database ID Description E-Value
Pfam IPR002619 EMBL-EBI Protein of unknown function CX 4.5E-37
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741583.1 1 198-217
NP_741584.1 1 198-217
F21C10.3a 1 198-217
F21C10.3b 1 198-217
SNAP00000042382 1 198-217
GENEFINDER00000042384 1 198-217
Sequence
Target Sequence 
>C. ELEGANS:186421_AT
ttcgttccggtgcacatggctataaagtacaaacaaaaacgtctacattttctaattacg
ggtcctcatcatttcgatctgcacatttgtatcattctccacatctccagaaacggccgg
gtcatccgtttgtcattatcgctgctaccacaccattattttacgacaatcgaaattact
actggagctatggtttggcaaagtctacacaaaactcgtcatatccaggagttatctgcg
agtatgtatttggagaggatgatggagaattgcaaaatataactttcgcaaatgcaactc
aagtaaagtcaatattcttcagttgctcaggaagagttgattgttgtggaatgtactgct
gtcacgatttcaatcaatggtttgagctagcatttgtctttggattcattgctgtgatgt
gtttcattgcctattacgccaagaaaaatgaaagtgaacgcccagaagtgccaaaagatt
tggagcctctacgacccaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCGTTCCGGTGCACATGGCTATAA 698 685 227 Antisense
TAATTACGGGTCCTCATCATTTCGA 356 635 279 Antisense
ATCATTTCGATCTGCACATTTGTAT 144 31 294 Antisense
GTCATCCGTTTGTCATTATCGCTGC 508 467 347 Antisense
GCTACCACACCATTATTTTACGACA 524 303 370 Antisense
GTCTACACAAAACTCGTCATATCCA 281 469 429 Antisense
TGTACTGCTGTCACGATTTCAATCA 234 559 578 Antisense
GAGCTAGCATTTGTCTTTGGATTCA 663 385 610 Antisense
GATGTGTTTCATTGCCTATTACGCC 253 409 642 Antisense
GAAAGTGAACGCCCAGAAGTGCCAA 352 361 676 Antisense
AGATTTGGAGCCTCTACGACCCAAA 64 65 702 Antisense
Sequence Source Affymetrix Proprietary Database