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[186405_at]

Description H34I24.1 /REP_DB=WormBase Gene ID /WP=CE19501 /TR=O76673 /GB=AAK18966.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 87      4grp_total > 10      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 110
1 2 3 4 range
A 4.5
(-3)
A 		49.7
(-0.5)
A 		12.5
(-2.6)
A 		114.2
(3)
P 		110
B 11.7
(-2.3)
A 		4.7
(-3)
A 		5.1
(-3)
A 		62.7
(3)
A 		58
C 4.6
(-3)
A 		12.6
(-2.2)
A 		23.2
(-1.1)
A 		63.2
(3)
A 		59
avr 6.9
(-3) 		22.3
(-1.7) 		13.6
(-2.5) 		80
(3) 		73

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 72
1 2 3 4 5 6 range
A 16
(-2.4)
A 		10.2
(-3)
A 		10.2
(-3)
A 		19.4
(-2)
A 		17.1
(-2.3)
A 		68.3
(3)
A 		58
B 10.1
(-3)
A 		20.5
(-2.1)
A 		15.5
(-2.5)
A 		11.6
(-2.9)
A 		72.2
(2.3)
A 		80.4
(3)
A 		70
C 11.6
(-2.7)
A 		8.5
(-3)
A 		18.2
(-2.2)
A 		43.5
(-0)
A 		30.5
(-1.1)
A 		78.9
(3)
M 		70
avr 12.6
(-3) 		13.1
(-3) 		14.6
(-2.8) 		24.8
(-1.8) 		39.9
(-0.4) 		75.9
(3) 		63
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 1.6
(-3)
A 		10.8
(0.7)
A 		3.3
(-2.3)
A 		16.7
(3)
P 		15
B 3.7
(-1.8)
A 		1.2
(-3)
A 		1.2
(-3)
A 		13.4
(3)
A 		12
C 1.5
(-3)
A 		3.6
(-1.6)
A 		8.1
(1.3)
A 		10.7
(3)
A 		9
avr 2.3
(-3) 		5.2
(-1.5) 		4.2
(-2) 		13.6
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 2.9
(-2.7)
A 		3.6
(-2.4)
A 		2.2
(-3)
A 		4.9
(-1.9)
A 		5.2
(-1.8)
A 		16.6
(3)
A 		14
B 3.5
(-2.9)
A 		6.8
(-2.2)
A 		3.8
(-2.8)
A 		3.1
(-3)
A 		29.3
(2.8)
A 		30
(3)
A 		27
C 4.3
(-2.6)
A 		2.6
(-3)
A 		4.6
(-2.5)
A 		8.8
(-1.4)
A 		9.9
(-1.2)
A 		26.4
(3)
M 		24
avr 3.6
(-3) 		4.3
(-2.8) 		3.5
(-3) 		5.6
(-2.4) 		14.8
(0.3) 		24.3
(3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186405_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7750
Sequence Type Exemplar sequence
Representative Public ID H34I24.1 NCBI
Target Description H34I24.1 /REP_DB=WormBase Gene ID /WP=CE19501 /TR=O76673 /GB=AAK18966.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065228(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065228 NCBI Caenorhabditis elegans H34I24.1 (H34I24.1) mRNA, complete cds. 11/11 None
SNAP00000044590 ENSEMBL cdna:SNAP chromosome:CEL140:III:2842419:2848543:1 11/11 None
GENEFINDER00000044593 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2847051:2848543:1 11/11 None
H34I24.1 ENSEMBL cdna:known chromosome:CEL140:III:2847051:2848543:1 gene:H34I24.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2847050-2848543(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H34I24.1
Entrez Gene 175402 Entrez gene
SwissProt O76673 EMBL-EBI
WormBase CE19501 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK18967 Hypothetical protein H34I24.2 [Caenorhabditis elegans] ref|NP_497628.1| H34I24.2 [Caenorhabditis elegans] 0.0
blast AAK18966 Hypothetical protein H34I24.1 [Caenorhabditis elegans] ref|NP_497629.1| H34I24.1 [Caenorhabditis elegans] 1.0E-120
blast AAK18966 Hypothetical protein H34I24.1 [Caenorhabditis elegans] ref|NP_497629.1| H34I24.1 [Caenorhabditis elegans] 1.0E-119
blast CAE57498 Hypothetical protein CBG00470 [Caenorhabditis briggsae] 4.0E-70
blast AAF59547 Hypothetical protein Y53G8AR.1 [Caenorhabditis elegans] ref|NP_497692.1| Y53G8AR.1 [Caenorhabditis elegans] 2.0E-16
blast CAA21609 Hypothetical protein Y43F8C.5 [Caenorhabditis elegans] ref|NP_507805.1| Y43F8C.5 [Caenorhabditis elegans] 3.0E-15
Sequence
Target Sequence 
>C. ELEGANS:186405_AT
agatccccacgttcgatgaaattctgaagatatatgagaatcgagcggaaaaaataattg
ataaggcaacacaaggaaatacaaagctttgcgagaatgacccgtattgttatggacaac
gaattctgaatcccggacaaaaaactatcaaatgcaaatatgaatgcaaggaagagggcg
ctggagatgattgtgcggattacaagttgatttgtaacctctatccgttgcccgaatcgg
ctgacttgtggtggaataagcaaaccaatggagaagctggctcagcgtgcgatgctgatc
gtcacgctgacaatggtctctgtgcagacggtgctccaacgaccactacaacgactacag
tgaagccaccgactactgctccagctgtgcagtcgaattctagagatgtgggagaactct
caacttctgccactaataactatcttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGATCCCCACGTTCGATGAAATTCT 371 59 305 Antisense
AAGCTTTGCGAGAATGACCCGTATT 695 149 388 Antisense
TTGATTTGTAACCTCTATCCGTTGC 268 705 511 Antisense
CCCGAATCGGCTGACTTGTGGTGGA 652 259 535 Antisense
GAGAAGCTGGCTCAGCGTGCGATGC 687 391 575 Antisense
CGTGCGATGCTGATCGTCACGCTGA 478 245 590 Antisense
GTCACGCTGACAATGGTCTCTGTGC 47 467 605 Antisense
TACAGTGAAGCCACCGACTACTGCT 346 643 660 Antisense
CGACTACTGCTCCAGCTGTGCAGTC 664 251 674 Antisense
TGTGGGAGAACTCTCAACTTCTGCC 210 555 711 Antisense
ACTTCTGCCACTAATAACTATCTTC 301 103 727 Antisense
Sequence Source Affymetrix Proprietary Database