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[186394_s_at]

Description F31D5.3B /REP_DB=WormBase Gene ID /WP=CE19828 /TR=Q19929 /GB=AAC71103.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 93      6grp_high1_10_10 > 49      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 85      6grp_total > 49      4grp_total > 04      6grp_highest_sub > Cu      6grp_cgc > 09      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1792
1 2 3 4 range
A 808.4
(-2.1)
P 		609.5
(-2.8)
P 		550.5
(-3)
P 		2328.8
(3)
P 		1778
B 975.8
(-1.5)
P 		630.9
(-3)
P 		728.8
(-2.6)
P 		2035
(3)
P 		1404
C 774.3
(-1.9)
P 		536.7
(-3)
P 		581.1
(-2.8)
P 		1858.6
(3)
P 		1322
avr 852.8
(-1.9) 		592.4
(-3) 		620.1
(-2.9) 		2074.1
(3) 		1482

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 845
1 2 3 4 5 6 range
A 971.2
(0.7)
P 		930.5
(0.3)
P 		546.6
(-3)
P 		708.1
(-1.6)
P 		804.7
(-0.8)
P 		1241.1
(3)
P 		695
B 776.5
(1.5)
P 		698.1
(0.6)
P 		396.6
(-3)
P 		707.8
(0.7)
P 		633.5
(-0.2)
P 		901.7
(3)
P 		505
C 887.5
(0.8)
P 		559
(-3)
P 		779.7
(-0.5)
P 		579.8
(-2.8)
P 		1078.6
(3)
P 		682
(-1.6)
P 		520
avr 878.4
(2) 		729.2
(-0.5) 		574.3
(-3) 		665.2
(-1.5) 		838.9
(1.3) 		941.6
(3) 		367
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 301
1 2 3 4 range
A 294.3
(1.7)
P 		132.3
(-3)
P 		143.9
(-2.7)
P 		340.9
(3)
P 		209
B 311.2
(0.4)
P 		155.8
(-3)
P 		168.9
(-2.7)
P 		433.6
(3)
P 		278
C 256.3
(0.8)
P 		154.3
(-3)
P 		202.5
(-1.2)
P 		313.4
(3)
P 		159
avr 287.3
(0.9) 		147.5
(-3) 		171.8
(-2.3) 		362.6
(3) 		215

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 253
1 2 3 4 5 6 range
A 179
(-1.3)
P 		330.9
(3)
P 		119
(-3)
P 		179.6
(-1.3)
P 		242.8
(0.5)
P 		302.2
(2.2)
P 		212
B 270.2
(1.3)
P 		232.4
(0.4)
P 		96.3
(-3)
P 		187.5
(-0.7)
P 		257.4
(1)
P 		336.3
(3)
P 		240
C 329
(2.5)
P 		170.6
(-1.6)
P 		198.4
(-0.9)
P 		116.6
(-3)
P 		349
(3)
P 		228.3
(-0.1)
P 		232
avr 259.4
(1.8) 		244.6
(1.2) 		137.9
(-3) 		161.2
(-2.1) 		283.1
(2.8) 		288.9
(3) 		151
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186394_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4393
Sequence Type Exemplar sequence
Representative Public ID F31D5.3B NCBI
Target Description F31D5.3B /REP_DB=WormBase Gene ID /WP=CE19828 /TR=Q19929 /GB=AAC71103.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062335(11), NM_062336(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062335 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-149) (tag-149) mRNA, complete cds. 11/11 None
NM_062336 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-149) (tag-149) mRNA, complete cds. 11/11 None
SNAP00000018590 ENSEMBL cdna:SNAP chromosome:CEL140:II:4204808:4208260:1 11/11 None
GENEFINDER00000018596 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4206464:4208260:1 11/11 None
F31D5.3b ENSEMBL cdna:known chromosome:CEL140:II:4186660:4208480:1 gene:F31D5.3 11/11 None
F31D5.3a ENSEMBL cdna:known chromosome:CEL140:II:4186670:4208260:1 gene:F31D5.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182186 3/11 Cross Hyb Matching Probes None
F31D5.3d 3/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173753 4 2 NM_062335 186394_s_at (11)
NM_062336 186394_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4186668-4208259(+) 100.0 100.0
Public Domain and Genome References
Ensembl F31D5.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC71102 Temporarily assigned gene name protein 149, isoform b [Caenorhabditis elegans] ref|NP_494736.1| Temporarily Assigned Gene name family member (tag-149) [Caenorhabditis elegans] 0.0
blast AAC71103 Temporarily assigned gene name protein 149, isoform a [Caenorhabditis elegans] ref|NP_494737.1| Temporarily Assigned Gene name family member (tag-149) [Caenorhabditis elegans] 0.0
blast CAE74278 Hypothetical protein CBG21973 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR010734 EMBL-EBI Copine 7.5E-73
Pfam IPR010734 EMBL-EBI Copine 7.5E-73
Pfam IPR010734 EMBL-EBI Copine 7.5E-73
Pfam IPR010734 EMBL-EBI Copine 7.5E-73
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494736.1 1 23-45
NP_494737.1 1 23-45
F31D5.3b 1 23-45
F31D5.3a 1 23-45
Sequence
Target Sequence 
>C. ELEGANS:186394_S_AT
ttggaaggacgttggctcctttcgccgggcagggaagacttggagtctatggattcggag
acgcaaagactggagactggagtgtattcaatttgaagggcgaaggaggagattgtagaa
gtttggatgaggtgttgaatgtttataatacagtcacaccaactgtagcactctccggac
cgactaactttgcccctctcatctatcaggcaatggagatttgtcaaaagtccagagatt
accacatccttgtaatcattgccgatgggcaagtgacaaatgagcgagcaactcgaagag
caattgtacaggcctgccagcatcctctctcgattattgtcgtcggtgttggagatggtc
catgggatatgatgaggatattcgacgaatcccttccaaaacgaccatgggacaacttcc
actttgtcgagttccacgaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGAAGGACGTTGGCTCCTTTCGC 160 693 2726 Antisense
CTCCTTTCGCCGGGCAGGGAAGACT 190 231 2741 Antisense
CAGTCACACCAACTGTAGCACTCTC 638 207 2876 Antisense
TTGCCCCTCTCATCTATCAGGCAAT 492 701 2915 Antisense
ACCACATCCTTGTAATCATTGCCGA 344 85 2966 Antisense
GCAATTGTACAGGCCTGCCAGCATC 584 321 3025 Antisense
GCATCCTCTCTCGATTATTGTCGTC 660 309 3045 Antisense
TATTGTCGTCGGTGTTGGAGATGGT 454 663 3060 Antisense
CGAATCCCTTCCAAAACGACCATGG 77 253 3111 Antisense
ACGACCATGGGACAACTTCCACTTT 427 83 3126 Antisense
TTCCACTTTGTCGAGTTCCACGAGA 129 701 3142 Antisense
Sequence Source Affymetrix Proprietary Database