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[186392_at]

Description T24A6.15 /REP_DB=WormBase Gene ID /WP=CE14152 /TR=O61947 /GB=AAC17802.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 80      4grp_total > 12      6grp_cgc > 85      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 100
1 2 3 4 range
A 38.1
(-2.8)
A 		83.9
(3)
P 		36.9
(-3)
A 		68.2
(1)
A 		47
B 70.8
(3)
P 		14.3
(-2.3)
A 		6.3
(-3)
A 		40
(0.1)
A 		65
C 16.3
(-3)
A 		18.4
(-2.9)
A 		41.4
(-1.3)
A 		106.4
(3)
A 		90
avr 41.7
(-1.1) 		38.9
(-1.5) 		28.2
(-3) 		71.5
(3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 72
1 2 3 4 5 6 range
A 50.9
(2.8)
A 		16.6
(-3)
A 		52
(3)
A 		33.3
(-0.2)
A 		29.6
(-0.8)
A 		17.6
(-2.8)
A 		35
B 18.9
(-1.1)
A 		28
(0.4)
A 		39.2
(2.2)
A 		39
(2.1)
A 		44.4
(3)
P 		7.1
(-3)
A 		37
C 34.6
(-0.5)
A 		15.8
(-2.1)
A 		5.3
(-3)
A 		76.8
(3)
A 		32.7
(-0.7)
A 		40.9
(-0)
P 		72
avr 34.8
(-0) 		20.1
(-3) 		32.2
(-0.5) 		49.7
(3) 		35.6
(0.1) 		21.9
(-2.6) 		30
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 13.9
(-0)
A 		18.2
(3)
P 		9.7
(-3)
A 		10
(-2.8)
A 		9
B 22.6
(3)
P 		3.5
(-2.4)
A 		1.5
(-3)
A 		8.5
(-1)
A 		21
C 5.4
(-3)
A 		5.3
(-3)
A 		14.4
(1.3)
A 		17.9
(3)
A 		13
avr 14
(3) 		9
(-2.5) 		8.5
(-3) 		12.1
(0.9) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 9.4
(1.4)
A 		5.9
(-1.6)
A 		11.3
(3)
A 		8.4
(0.5)
A 		8.9
(0.9)
A 		4.3
(-3)
A 		7
B 6.6
(-1.5)
A 		9.3
(-0.4)
A 		9.5
(-0.3)
A 		10.3
(-0)
A 		18
(3)
P 		2.7
(-3)
A 		15
C 12.8
(1.9)
A 		4.8
(-1.5)
A 		1.4
(-3)
A 		15.4
(3)
A 		10.6
(0.9)
A 		13.7
(2.3)
P 		14
avr 9.6
(-0) 		6.7
(-3) 		7.4
(-2.3) 		11.4
(1.9) 		12.5
(3) 		6.9
(-2.8) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186392_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15486
Sequence Type Exemplar sequence
Representative Public ID T24A6.15 NCBI
Target Description T24A6.15 /REP_DB=WormBase Gene ID /WP=CE14152 /TR=O61947 /GB=AAC17802.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071594(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071594 NCBI Caenorhabditis elegans GRound-Like (grd related) family member (grl-28) (grl-28) mRNA, complete cds. 11/11 None
SNAP00000027428 ENSEMBL cdna:SNAP chromosome:CEL140:V:3551172:3551691:-1 11/11 None
GENEFINDER00000027445 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3551172:3551691:-1 11/11 None
T24A6.15 ENSEMBL cdna:known chromosome:CEL140:V:3551172:3551691:-1 gene:T24A6.15 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3551171-3551691(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol grl-28
Ensembl T24A6.15
Entrez Gene 188832 Entrez gene
SwissProt O61947 EMBL-EBI
WormBase CE14152 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC17802 Ground-like (grd related) protein 28 [Caenorhabditis elegans] ref|NP_503995.1| GRound-Like (grd related) family member (grl-28) [Caenorhabditis elegans] 9.0E-53
blast AAF39799 Ground-like (grd related) protein 27 [Caenorhabditis elegans] ref|NP_504006.1| GRound-Like (grd related) family member (grl-27) [Caenorhabditis elegans] 5.0E-39
blast CAE58215 Hypothetical protein CBG01310 [Caenorhabditis briggsae] 2.0E-36
Protein Domains
Database ID Description E-Value
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 1.9E-23
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503995.1 1 5-27
T24A6.15 1 5-27
SNAP00000027428 1 5-27
GENEFINDER00000027445 1 5-27
Sequence
Target Sequence 
>C. ELEGANS:186392_AT
gctgtggatgtggagcaccaccgccaccatcctgtggctgcggaggacggaaaaagagat
ctcttccggaaaagccgacattttataaaatttctgcttcaggtgaagatttggtgtgta
acactccggaattgaagaaaattatagtggagaacatggaatcttccgcaatagaatctt
caaaatctgtaaatgcagtactggaaacccgccagctcaaccgattcgtggtggtgtgct
ccgagaatccgtttgctttcacagcccgcgccgactcagcgtactgtggagctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTGTGGATGTGGAGCACCACCGCC 539 295 101 Antisense
CATCCTGTGGCTGCGGAGGACGGAA 418 211 128 Antisense
AAGAGATCTCTTCCGGAAAAGCCGA 5 155 154 Antisense
GAAGATTTGGTGTGTAACACTCCGG 345 337 205 Antisense
AGTGGAGAACATGGAATCTTCCGCA 299 59 246 Antisense
GAATCTTCCGCAATAGAATCTTCAA 153 329 259 Antisense
ATGCAGTACTGGAAACCCGCCAGCT 194 41 293 Antisense
GCTCAACCGATTCGTGGTGGTGTGC 469 301 315 Antisense
TTCGTGGTGGTGTGCTCCGAGAATC 236 669 325 Antisense
TCCGAGAATCCGTTTGCTTTCACAG 662 595 340 Antisense
GCCGACTCAGCGTACTGTGGAGCTG 420 281 370 Antisense
Sequence Source Affymetrix Proprietary Database