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[186377_at]

Description F35H10.7 /REP_DB=WormBase Gene ID /WP=CE04507 /TR=Q20069 /GB=AAA81679.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 88      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_highest_nonorm > 94      6grp_total > 88      4grp_total > 52      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 219
1 2 3 4 range
A 582.4
(3)
P 		564.2
(1.8)
P 		505.7
(-2.2)
P 		493.4
(-3)
P 		89
B 443.3
(-3)
P 		566
(3)
P 		520.2
(0.8)
P 		459.6
(-2.2)
P 		123
C 638.2
(3)
P 		525.6
(-0.1)
P 		526.3
(-0.1)
P 		418.9
(-3)
P 		219
avr 554.6
(3) 		551.9
(2.8) 		517.4
(0.7) 		457.3
(-3) 		97

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 423
1 2 3 4 5 6 range
A 568.2
(0.5)
P 		403.3
(-3)
P 		411.5
(-2.8)
P 		505
(-0.8)
P 		549
(0.1)
P 		684.4
(3)
P 		281
B 495.7
(-1.4)
P 		388.3
(-3)
P 		447.5
(-2.1)
P 		428.4
(-2.4)
P 		674.7
(1.4)
P 		780.6
(3)
P 		392
C 357.6
(-3)
P 		459.3
(-1.5)
P 		481.6
(-1.2)
P 		462.1
(-1.5)
P 		589.5
(0.4)
P 		765
(3)
P 		407
avr 473.8
(-2) 		417
(-3) 		446.9
(-2.5) 		465.2
(-2.1) 		604.4
(0.4) 		743.3
(3) 		326
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 141
1 2 3 4 range
A 212
(3)
P 		122.5
(-0.8)
P 		132.2
(-0.4)
P 		72.2
(-3)
P 		140
B 141.4
(3)
P 		139.8
(2.8)
P 		120.5
(0.1)
P 		97.9
(-3)
P 		44
C 211.3
(3)
P 		151.1
(0.4)
P 		183.4
(1.8)
P 		70.6
(-3)
P 		141
avr 188.2
(3) 		137.8
(0.2) 		145.4
(0.6) 		80.2
(-3) 		108

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 202
1 2 3 4 5 6 range
A 104.7
(-1.8)
P 		143.4
(1.2)
P 		89.6
(-3)
P 		128.1
(0)
P 		165.7
(2.9)
P 		166.6
(3)
P 		77
B 172.5
(-0.9)
P 		129.3
(-2.3)
P 		108.7
(-3)
P 		113.5
(-2.8)
P 		274.1
(2.4)
P 		291.1
(3)
P 		182
C 132.5
(-1.5)
P 		140.2
(-1.3)
P 		122.6
(-1.9)
P 		92.9
(-3)
P 		190.8
(0.6)
P 		256.1
(3)
P 		163
avr 136.6
(-1.6) 		137.6
(-1.6) 		107
(-3) 		111.5
(-2.8) 		210.2
(1.7) 		237.9
(3) 		131
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186377_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9072
Sequence Type Exemplar sequence
Representative Public ID F35H10.7 NCBI
Target Description F35H10.7 /REP_DB=WormBase Gene ID /WP=CE04507 /TR=Q20069 /GB=AAA81679.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068995(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068995 NCBI Caenorhabditis elegans F35H10.7 (F35H10.7) mRNA, complete cds. 11/11 None
SNAP00000039423 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8299171:8299750:1 9/11 None
GENEFINDER00000039438 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8297475:8299750:1 11/11 None
F35H10.7.2 ENSEMBL cdna:known chromosome:CEL140:IV:8297471:8299809:1 gene:F35H10.7 11/11 None
F35H10.7.1 ENSEMBL cdna:known chromosome:CEL140:IV:8297474:8299822:1 gene:F35H10.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000039422 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8297480-8299756(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35H10.7
Entrez Gene 185331 Entrez gene
SwissProt Q20069 EMBL-EBI
WormBase CE04507 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1639212_A_AT dm
DROSGENOME1:142760_AT dm
CHICKEN:GGAAFFX.4649.1.S1_AT CGTHBA gga
HG-U133_PLUS_2:214273_X_AT chromosome 16 open reading frame 35 hs
HG-U133A_2:214273_X_AT chromosome 16 open reading frame 35 hs
HG-U133A:214273_X_AT chromosome 16 open reading frame 35 hs
HG-U133_PLUS_2:210672_S_AT chromosome 16 open reading frame 35 hs
HG-U133A:210672_S_AT chromosome 16 open reading frame 35 hs
HG-U133A_2:210672_S_AT chromosome 16 open reading frame 35 hs
HUGENEFL:Z84722_AT chromosome 16 open reading frame 35 hs
HUGENEFL:X90857_AT chromosome 16 open reading frame 35 hs
U133_X3P:G13278839_3P_A_AT chromosome 16 open reading frame 35 hs
U133_X3P:HS.19699.4.A1_3P_A_AT chromosome 16 open reading frame 35 hs
U133_X3P:HS.19699.0.A1_3P_A_AT chromosome 16 open reading frame 35 hs
HG-U133A_2:203289_S_AT chromosome 16 open reading frame 35 hs
HG-FOCUS:203289_S_AT chromosome 16 open reading frame 35 hs
HG-U133_PLUS_2:203289_S_AT chromosome 16 open reading frame 35 hs
HG-U133A:203289_S_AT chromosome 16 open reading frame 35 hs
HG-U95AV2:40956_AT chromosome 16 open reading frame 35 hs
HG-U133_PLUS_2:229122_X_AT Chromosome 16 open reading frame 35 hs
HG-U133B:229122_X_AT Chromosome 16 open reading frame 35 hs
HU35KSUBA:W67675_AT Chromosome 16 open reading frame 35 hs
HU35KSUBB:RC_T58648_AT Chromosome 16 open reading frame 35 hs
U133_X3P:HS.19699.3.A1_3P_AT Chromosome 16 open reading frame 35 hs
MG-U74BV2:114440_AT alpha globin regulatory element containing gene mm
MOE430A:1433548_AT alpha globin regulatory element containing gene mm
MOUSE430A_2:1433548_AT alpha globin regulatory element containing gene mm
MOUSE430_2:1433548_AT alpha globin regulatory element containing gene mm
RG-U34B:RC_AA901352_AT similar to CGTHBA protein (-14 gene protein) (predicted) rn
RAE230A:1373948_AT similar to CGTHBA protein (-14 gene protein) (predicted) rn
RAT230_2:1373948_AT similar to CGTHBA protein (-14 gene protein) (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAA81679 Hypothetical protein F35H10.7 [Caenorhabditis elegans] ref|NP_501396.1| F35H10.7 [Caenorhabditis elegans] sp|Q20069|U171_CAEEL Hypothetical UPF0171 protein F35H10.7 in chromosome IV 0.0
blast CAE58451 Hypothetical protein CBG01588 [Caenorhabditis briggsae] 0.0
blast AAA81679 Hypothetical protein F35H10.7 [Caenorhabditis elegans] ref|NP_501396.1| F35H10.7 [Caenorhabditis elegans] sp|Q20069|U171_CAEEL Hypothetical UPF0171 protein F35H10.7 in chromosome IV 1.0E-87
blast CAE58451 Hypothetical protein CBG01588 [Caenorhabditis briggsae] 2.0E-67
Protein Domains
Database ID Description E-Value
Pfam IPR005365 EMBL-EBI Protein of unknown function UPF0171 1.0E-126
Pfam IPR005365 EMBL-EBI Protein of unknown function UPF0171 3.2E-96
Sequence
Target Sequence 
>C. ELEGANS:186377_AT
acacgtgctattctcatttatcctttatgtaatacaaacatctatacatctgcaacaagt
ccacaacctcttgataaaatggcagagaagttcacagctcaattcggcaataccattcac
ttggctgccgggctggctcatttcaatcccccaaaaacactggatacgtttatccgaaaa
aaccttcctctacacgaacagggagttcgtgcgaagcttgtcgttgctttgcttcgtcat
caaatgctcatgcagttgcatcaattttactatattctgaaaccgtactcgatcgcgatc
ctaccagaaccaaaagagccatgtcccgctgagtttacgaaaatcatcgaggaaagcaca
ttaccaaatgacgtgaagggagttgtagcggatatttgtgctgaaatgctcgaaactaca
tcttatgccagtgtcaaacgaacgttgagccttttcgtaaaagttgctccgatgatggat
ggaaatcatcatttggaagaaatcaaatacaaaaataatttggatcgtacagaaatcgag
ggagttttcgtgtctttcaagcttgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACACGTGCTATTCTCATTTATCCTT 90 111 943 Antisense
TACATCTGCAACAAGTCCACAACCT 135 643 987 Antisense
GTTCACAGCTCAATTCGGCAATACC 182 445 1032 Antisense
GGCAATACCATTCACTTGGCTGCCG 241 535 1048 Antisense
AACCTTCCTCTACACGAACAGGGAG 674 93 1123 Antisense
GAGTTCGTGCGAAGCTTGTCGTTGC 424 397 1145 Antisense
TCGTTGCTTTGCTTCGTCATCAAAT 83 605 1163 Antisense
TCTGAAACCGTACTCGATCGCGATC 241 607 1218 Antisense
TCGCGATCCTACCAGAACCAAAAGA 50 599 1235 Antisense
CCATGTCCCGCTGAGTTTACGAAAA 480 271 1261 Antisense
GAGTTTTCGTGTCTTTCAAGCTTGT 673 395 1484 Antisense
Sequence Source Affymetrix Proprietary Database