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[186373_at]

Description ZK1248.7 /REP_DB=WormBase Gene ID /WP=CE02907 /TR=Q23415 /GB=AAC71088.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 60      4grp_total > 20      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 65
1 2 3 4 range
A 15.4
(-2.1)
A 		13.7
(-2.4)
A 		11
(-3)
A 		40.4
(3)
A 		29
B 27.3
(3)
A 		4.7
(-2.9)
A 		11.8
(-1.1)
A 		4.5
(-3)
A 		23
C 17.5
(-1.9)
A 		42.1
(0.4)
A 		6.2
(-3)
A 		69.7
(3)
A 		64
avr 20.1
(-0.8) 		20.2
(-0.8) 		9.7
(-3) 		38.2
(3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 50.5
(3)
A 		3.1
(-3)
A 		25.1
(-0.2)
A 		6.3
(-2.6)
A 		25.6
(-0.2)
A 		3.8
(-2.9)
A 		47
B 23.5
(1.1)
A 		12.4
(-1.2)
A 		3.4
(-3)
A 		5.6
(-2.5)
A 		7.8
(-2.1)
A 		32.7
(3)
A 		29
C 4.1
(-2.9)
A 		38.7
(-0.2)
A 		34.6
(-0.5)
A 		78.1
(3)
A 		12.2
(-2.3)
A 		3.2
(-3)
A 		75
avr 26
(1.6) 		18.1
(-1.3) 		21
(-0.2) 		30
(3) 		15.2
(-2.3) 		13.2
(-3) 		17
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 5.6
(2.4)
A 		3
(-2.8)
A 		2.9
(-3)
A 		5.9
(3)
A 		3
B 8.7
(3)
A 		1.2
(-2.8)
A 		2.7
(-1.7)
A 		1
(-3)
A 		8
C 5.8
(-0.8)
A 		12.1
(3)
A 		2.2
(-3)
A 		11.8
(2.8)
A 		10
avr 6.7
(3) 		5.4
(1.1) 		2.6
(-3) 		6.2
(2.3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 9.3
(3)
A 		1.1
(-2.9)
A 		5.5
(0.3)
A 		1.6
(-2.5)
A 		7.7
(1.9)
A 		0.9
(-3)
A 		8
B 8.2
(0.9)
A 		4.1
(-1.3)
A 		0.8
(-3)
A 		1.5
(-2.6)
A 		3.2
(-1.7)
A 		12.2
(3)
A 		11
C 1.5
(-2.8)
A 		11.8
(1.4)
A 		8.8
(0.2)
A 		15.7
(3)
A 		3.9
(-1.8)
A 		1.1
(-3)
A 		15
avr 6.3
(3) 		5.7
(0.8) 		5
(-1.9) 		6.3
(3) 		4.9
(-2.3) 		4.7
(-3) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186373_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5312
Sequence Type Exemplar sequence
Representative Public ID ZK1248.7 NCBI
Target Description ZK1248.7 /REP_DB=WormBase Gene ID /WP=CE02907 /TR=Q23415 /GB=AAC71088.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062750(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062750 NCBI Caenorhabditis elegans ZK1248.7 (ZK1248.7) mRNA, complete cds. 11/11 None
SNAP00000041372 ENSEMBL cdna:SNAP chromosome:CEL140:II:5812340:5816368:-1 11/11 None
GENEFINDER00000041390 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5812340:5815446:-1 11/11 None
ZK1248.7 ENSEMBL cdna:known chromosome:CEL140:II:5812462:5813693:-1 gene:ZK1248.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5812458-5813690(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK1248.7
Entrez Gene 191545 Entrez gene
SwissProt Q23415 EMBL-EBI
WormBase CE02907 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC71088 Hypothetical protein ZK1248.7 [Caenorhabditis elegans] ref|NP_495151.1| ZK1248.7 [Caenorhabditis elegans] 0.0
blast CAA95839 Hypothetical protein R06C7.1 [Caenorhabditis elegans] ref|NP_492045.1| R06C7.1 [Caenorhabditis elegans] sp|Q21770|GCC7_CAEEL Germ cell expressed protein R06C7.1 0.0
blast AAB54129 Hypothetical protein F55A12.1 [Caenorhabditis elegans] ref|NP_491579.1| F55A12.1 [Caenorhabditis elegans] 0.0
blast CAE66929 Hypothetical protein CBG12321 [Caenorhabditis briggsae] 0.0
blast CAA95839 Hypothetical protein R06C7.1 [Caenorhabditis elegans] ref|NP_492045.1| R06C7.1 [Caenorhabditis elegans] sp|Q21770|GCC7_CAEEL Germ cell expressed protein R06C7.1 1.0E-178
Protein Domains
Database ID Description E-Value
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 1.5E-34
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 8.2E-23
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:186373_AT
ttttggccccttcaggacaggatacaatggcatcaattgaagacgttttgaaaagttcca
tcgatctgtttgagatgaatcgcaatactttgccaaagcggatcatcatttatagaagtg
gtgcatcggatggctctcatgcgtctattcttgcctacgaaattccactggcacgtgcta
ccattcagggatactccaaagatatcaatctgatttacatcatcgtcaccaaggagcaca
gttacagatttttccgtgatcagcttcgttccggcggaaaagcgacagagatgaacatcc
caccgggtatcgtcttggatagcgctgtcaccaatcctgcatgcaagcaattcttcctca
atggacacacaactctgcaaggaactgccaagactcctctctacactattctggcagatg
actgtaacgctccgatggatcgccttgaggagctcacatacactctttgccatcaccatc
aaatcgtggcactgagcacttcaattccgactc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTTGGCCCCTTCAGGACAGGATAC 38 675 500 Antisense
AGTGGTGCATCGGATGGCTCTCATG 285 59 616 Antisense
GCCTACGAAATTCCACTGGCACGTG 660 277 652 Antisense
GCACGTGCTACCATTCAGGGATACT 245 315 670 Antisense
ATGAACATCCCACCGGGTATCGTCT 161 43 790 Antisense
TTGGATAGCGCTGTCACCAATCCTG 387 707 814 Antisense
GCATGCAAGCAATTCTTCCTCAATG 523 309 838 Antisense
TCCTCTCTACACTATTCTGGCAGAT 96 593 894 Antisense
GATGACTGTAACGCTCCGATGGATC 147 411 916 Antisense
GATGGATCGCCTTGAGGAGCTCACA 520 405 933 Antisense
GCACTGAGCACTTCAATTCCGACTC 216 315 988 Antisense
Sequence Source Affymetrix Proprietary Database