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[186358_at]

Description ZK546.16 /REP_DB=WormBase Gene ID /WP=CE07633 /TR=Q23526 /GB=AAA68735.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 58      6grp_high1_10_10 > 84      4grp_highest_8_8 > 43      4grp_high_8_8 > 32      6grp_highest_nonorm > 92      6grp_total > 75      4grp_total > 55      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 217.7
(-0.2)
P 		198.7
(-3)
P 		239.8
(3)
P 		198.6
(-3)
P 		41
B 258.4
(3)
P 		191.8
(-2.2)
P 		181.9
(-3)
P 		193.3
(-2.1)
P 		77
C 271
(3)
P 		207
(-3)
P 		211.7
(-2.6)
P 		222.7
(-1.5)
P 		64
avr 249
(3) 		199.2
(-3) 		211.1
(-1.6) 		204.9
(-2.3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 310
1 2 3 4 5 6 range
A 110.4
(-3)
P 		194.9
(-0.4)
P 		282.2
(2.2)
P 		307.6
(3)
P 		302
(2.8)
P 		296.2
(2.7)
P 		197
B 179.1
(-3)
P 		246
(-1)
P 		311.4
(0.9)
P 		256.1
(-0.8)
P 		334.8
(1.5)
P 		384.9
(3)
P 		206
C 163.5
(-3)
P 		217.3
(-1.7)
P 		281.3
(-0.3)
P 		330.6
(0.9)
P 		254.1
(-0.9)
P 		420.8
(3)
P 		257
avr 151
(-3) 		219.4
(-1.1) 		291.6
(0.9) 		298.1
(1.1) 		297
(1) 		367.3
(3) 		216
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 61
1 2 3 4 range
A 79.3
(3)
P 		43.1
(-1.3)
P 		62.7
(1)
P 		29.1
(-3)
P 		50
B 82.4
(3)
P 		47.4
(-2.1)
P 		42.1
(-2.9)
P 		41.2
(-3)
P 		41
C 89.7
(3)
P 		59.5
(-0.5)
P 		73.8
(1.2)
P 		37.6
(-3)
P 		52
avr 83.8
(3) 		50
(-1.2) 		59.5
(-0.1) 		36
(-3) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 123
1 2 3 4 5 6 range
A 20.3
(-3)
P 		69.3
(1.2)
P 		61.5
(0.5)
P 		78
(1.9)
P 		91.1
(3)
P 		72.1
(1.4)
P 		71
B 62.3
(-3)
P 		81.9
(-1.6)
P 		75.6
(-2)
P 		67.8
(-2.6)
P 		136
(2.4)
P 		143.5
(3)
P 		81
C 60.6
(-3)
P 		66.3
(-2.6)
P 		71.6
(-2.2)
P 		66.5
(-2.6)
P 		82.2
(-1.4)
P 		140.9
(3)
P 		80
avr 47.7
(-3) 		72.5
(-0.9) 		69.6
(-1.2) 		70.8
(-1.1) 		103.1
(1.7) 		118.8
(3) 		71
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186358_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5583
Sequence Type Exemplar sequence
Representative Public ID ZK546.16 NCBI
Target Description ZK546.16 /REP_DB=WormBase Gene ID /WP=CE07633 /TR=Q23526 /GB=AAA68735.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027354(10), NM_170985(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027354 NCBI Caenorhabditis elegans ZK546.2c (ZK546.2) mRNA, complete cds. 10/11 None
NM_170985 NCBI Caenorhabditis elegans ZK546.2a (ZK546.2) mRNA, complete cds. 10/11 A 
SNAP00000034951 ENSEMBL cdna:SNAP chromosome:CEL140:II:4946012:4947936:1 10/11 None
GENEFINDER00000034964 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4946012:4947936:1 10/11 None
ZK546.2c ENSEMBL cdna:known chromosome:CEL140:II:4946012:4948055:1 gene:ZK546.2 10/11 A 
ZK546.2a ENSEMBL cdna:known chromosome:CEL140:II:4946012:4947936:1 gene:ZK546.2 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173855 3 2 NM_001027354 186358_at (10)
NM_170985 186358_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4946009-4946831(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZK546.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAR12987 Hypothetical protein ZK546.2c [Caenorhabditis elegans] ref|NP_001022525.1| ZK546.2c [Caenorhabditis elegans] 0.0
blast AAR12986 Hypothetical protein ZK546.2a [Caenorhabditis elegans] ref|NP_740983.2| ZK546.2a [Caenorhabditis elegans] 0.0
blast T27904 hypothetical protein ZK546.2b - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.019
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0018
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0016
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.076
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.99
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0033
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0015
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 1.5E-4
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.66
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.99
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0033
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0015
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 1.5E-4
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.66
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0016
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.076
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.99
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0033
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0015
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 1.5E-4
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.66
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.99
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0033
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0015
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 1.5E-4
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.66
Sequence
Target Sequence 
>C. ELEGANS:186358_AT
ctacaatccaacagtgcgtgcacgccttgcaaatgcttctaaaacgcgagtcctgagcct
gaaggagtctgccctgcaccgtattcctgatgacatcaactccctggcgtgtcttgaagt
tttaaatgtctcacaaaacaaactgacagagcttcccgatctgtcacagtgtgtctcatt
aaagacagtagaggcgatagaaaaccaattcatcatcttcccagctggtgtgtgcaaatg
tccaaatctcgaaacatgcttgttaactgaaaatcggatcgagaaacttccagacgaaat
tcattcgctacgggcaattagcgtgatcctcaacaaaaaccggcttctctctctcaacac
cgctaatttgcttcgctgtgaacgcttgcgtgcagtaaacgtggacgataaccaactaaa
tcgcgacgaaattgaacaattccttgtgaatgcgccacgaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTACAATCCAACAGTGCGTGCACGC 576 225 27 Antisense
AATGCTTCTAAAACGCGAGTCCTGA 415 165 58 Antisense
GTCCTGAGCCTGAAGGAGTCTGCCC 434 475 76 Antisense
CTGCACCGTATTCCTGATGACATCA 552 237 100 Antisense
CAAACTGACAGAGCTTCCCGATCTG 490 191 165 Antisense
TCCCGATCTGTCACAGTGTGTCTCA 615 597 180 Antisense
CTTCCCAGCTGGTGTGTGCAAATGT 511 219 243 Antisense
GGGCAATTAGCGTGATCCTCAACAA 210 493 338 Antisense
TCAACACCGCTAATTTGCTTCGCTG 541 629 380 Antisense
TTTGCTTCGCTGTGAACGCTTGCGT 12 659 393 Antisense
AATTCCTTGTGAATGCGCCACGAGA 153 177 464 Antisense
Sequence Source Affymetrix Proprietary Database