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[186343_s_at]

Description Y54E5A.8A /REP_DB=WormBase Gene ID /WP=CE20319 /TR=Q9XTC0 /GB=CAA21663.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 72      4grp_highest_8_8 > 29      4grp_high_8_8 > 07      6grp_highest_nonorm > 64      6grp_total > 73      4grp_total > 05      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 337
1 2 3 4 range
A 746.1
(-3)
P 		766.5
(-2.6)
P 		914.1
(0.5)
P 		1037.9
(3)
P 		292
B 885.3
(1.8)
P 		700.5
(-3)
P 		747.4
(-1.8)
P 		929.6
(3)
P 		229
C 834.2
(-0.6)
P 		725.9
(-3)
P 		740.2
(-2.7)
P 		998.6
(3)
P 		273
avr 821.9
(-0.9) 		731
(-3) 		800.6
(-1.4) 		988.7
(3) 		258

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 374
1 2 3 4 5 6 range
A 791.9
(-2)
P 		788.7
(-2.1)
P 		769.7
(-2.7)
P 		951
(3)
P 		776
(-2.5)
P 		759.1
(-3)
P 		192
B 634.7
(-2.9)
P 		629.3
(-3)
P 		852.8
(3)
P 		854.2
(3)
P 		848.7
(2.9)
P 		730.8
(-0.3)
P 		225
C 728.4
(-1.9)
P 		668.8
(-3)
P 		1003.4
(3)
P 		978.4
(2.6)
P 		891.5
(1)
P 		766.4
(-1.2)
P 		335
avr 718.3
(-2.4) 		695.6
(-3) 		875.3
(1.6) 		927.9
(3) 		838.7
(0.7) 		752.1
(-1.5) 		232
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 130
1 2 3 4 range
A 271.7
(3)
P 		166.4
(-2.3)
P 		239
(1.4)
P 		151.9
(-3)
P 		120
B 282.3
(3)
P 		173
(-3)
P 		173.2
(-3)
P 		198.1
(-1.6)
P 		109
C 276.1
(3)
P 		208.7
(-0.8)
P 		258
(2)
P 		168.4
(-3)
P 		108
avr 276.7
(3) 		182.7
(-2.4) 		223.4
(-0.1) 		172.8
(-3) 		104

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 199
1 2 3 4 5 6 range
A 146
(-3)
P 		280.5
(3)
P 		167.6
(-2)
P 		241.2
(1.2)
P 		234.2
(0.9)
P 		184.8
(-1.3)
P 		135
B 220.8
(-2.4)
P 		209.5
(-2.9)
P 		207.1
(-3)
P 		226.3
(-2.2)
P 		344.8
(3)
P 		272.5
(-0.2)
P 		138
C 270
(1.8)
P 		204.1
(-2.5)
P 		255.4
(0.8)
P 		196.8
(-3)
P 		288.5
(3)
P 		256.6
(0.9)
P 		92
avr 212.3
(-2.8) 		231.4
(-1.4) 		210
(-3) 		221.4
(-2.1) 		289.2
(3) 		238
(-0.9) 		79
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186343_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1551
Sequence Type Exemplar sequence
Representative Public ID Y54E5A.8A NCBI
Target Description Y54E5A.8A /REP_DB=WormBase Gene ID /WP=CE20319 /TR=Q9XTC0 /GB=CAA21663.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061156(11), NM_061155(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061156 NCBI Caenorhabditis elegans Y54E5A.8b (Y54E5A.8) mRNA, complete cds. 11/11 None
NM_061155 NCBI Caenorhabditis elegans Y54E5A.8a (Y54E5A.8) mRNA, complete cds. 11/11 None
GENEFINDER00000018551 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14725684:14727103:1 11/11 None
Y54E5A.8b ENSEMBL cdna:known chromosome:CEL140:I:14724732:14727103:1 gene:Y54E5A.8 11/11 None
Y54E5A.8a ENSEMBL cdna:known chromosome:CEL140:I:14724805:14727136:1 gene:Y54E5A.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000018533 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14724778-14727034(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y54E5A.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA21663 Hypothetical protein Y54E5A.8a [Caenorhabditis elegans] emb|CAB04578.1| Hypothetical protein Y54E5A.8a [Caenorhabditis elegans] ref|NP_493556.1| Y54E5A.8a [Caenorhabditis elegans] 1.0E-141
blast CAA21664 Hypothetical protein Y54E5A.8b [Caenorhabditis elegans] emb|CAB04579.1| Hypothetical protein Y54E5A.8b [Caenorhabditis elegans] ref|NP_493557.1| Y54E5A.8b [Caenorhabditis elegans] 1.0E-131
blast CAA21663 Hypothetical protein Y54E5A.8a [Caenorhabditis elegans] emb|CAB04578.1| Hypothetical protein Y54E5A.8a [Caenorhabditis elegans] ref|NP_493556.1| Y54E5A.8a [Caenorhabditis elegans] 1.0E-121
blast CAA21664 Hypothetical protein Y54E5A.8b [Caenorhabditis elegans] emb|CAB04579.1| Hypothetical protein Y54E5A.8b [Caenorhabditis elegans] ref|NP_493557.1| Y54E5A.8b [Caenorhabditis elegans] 1.0E-121
blast CAA21663 Hypothetical protein Y54E5A.8a [Caenorhabditis elegans] emb|CAB04578.1| Hypothetical protein Y54E5A.8a [Caenorhabditis elegans] ref|NP_493556.1| Y54E5A.8a [Caenorhabditis elegans] 2.0E-81
blast CAA21664 Hypothetical protein Y54E5A.8b [Caenorhabditis elegans] emb|CAB04579.1| Hypothetical protein Y54E5A.8b [Caenorhabditis elegans] ref|NP_493557.1| Y54E5A.8b [Caenorhabditis elegans] 2.0E-81
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493557.1 1 134-156
NP_493556.1 2 13-30,154-176
Y54E5A.8b 1 134-156
Y54E5A.8a 2 13-30,154-176
GENEFINDER00000018551 1 52-74
Sequence
Target Sequence 
>C. ELEGANS:186343_S_AT
aaagccgtcgaaccaggtggctcacgtgtcatttctgcttcacttccaccaccgcctacg
cagaagaaagagcaaacgtctacgccaagttcctattccttcaagccatctgatttcccg
tggattcttgtcggattactcttctgcattggaattttcatgggcgtcatgcttaccgct
tgttaccattttataaataatatgcaaaaggagccacaattcgatcgttccagtgccatt
atatataataaagctggcgatagtgtgggctccgagagccgccgtccgatgcttcccagg
cggagagagcagcaaattgagcttatagaacgggcatctgtggcaccttctatgtcctct
ggaacacgaataggccctgcgtggcggaacactaatgggactccagctgttataatcacc
ac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGCCGTCGAACCAGGTGGCTCAC 13 125 349 Antisense
AGCAAACGTCTACGCCAAGTTCCTA 333 75 419 Antisense
TCAAGCCATCTGATTTCCCGTGGAT 114 631 449 Antisense
ATTCTTGTCGGATTACTCTTCTGCA 647 11 472 Antisense
GCTTACCGCTTGTTACCATTTTATA 400 305 519 Antisense
TTCGATCGTTCCAGTGCCATTATAT 571 693 568 Antisense
GGCGATAGTGTGGGCTCCGAGAGCC 66 543 604 Antisense
TATAGAACGGGCATCTGTGGCACCT 175 657 672 Antisense
ACCTTCTATGTCCTCTGGAACACGA 528 87 693 Antisense
GAACACGAATAGGCCCTGCGTGGCG 595 351 710 Antisense
GGACTCCAGCTGTTATAATCACCAC 389 525 746 Antisense
Sequence Source Affymetrix Proprietary Database