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[186328_at]

Description F56D12.5 /REP_DB=WormBase Gene ID /WP=CE11254 /TR=O16646 /GB=AAB66119.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 02      4grp_highest_8_8 > 22      4grp_high_8_8 > 15      6grp_total > 07      4grp_total > 01      6grp_cgc > 24      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 571
1 2 3 4 range
A 667.7
(-3)
P 		673.4
(-2.9)
P 		693.4
(-2.5)
P 		964.1
(3)
P 		296
B 702.8
(-1.2)
P 		543.7
(-3)
P 		794.4
(-0.2)
P 		1086.8
(3)
P 		543
C 725.7
(-3)
P 		904.7
(-0.2)
P 		805.5
(-1.8)
P 		1114.8
(3)
P 		389
avr 698.7
(-3) 		707.3
(-2.9) 		764.4
(-1.9) 		1055.2
(3) 		357

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 278
1 2 3 4 5 6 range
A 204.4
(-2.3)
P 		435.2
(3)
P 		207.4
(-2.2)
P 		315.2
(0.2)
P 		174.6
(-3)
P 		364.4
(1.4)
P 		261
B 233.5
(0.3)
P 		200.8
(-2.9)
P 		260.9
(3)
P 		229.1
(-0.1)
P 		217.2
(-1.3)
P 		199.3
(-3)
P 		62
C 452.7
(3)
P 		189.2
(-2.8)
P 		309
(-0.1)
P 		224.6
(-2)
A 		296.5
(-0.4)
P 		178.7
(-3)
P 		274
avr 296.9
(3) 		275.1
(1.1) 		259.1
(-0.4) 		256.3
(-0.6) 		229.4
(-3) 		247.5
(-1.4) 		68
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 147
1 2 3 4 range
A 243.1
(3)
P 		146.2
(-2.7)
P 		181.3
(-0.6)
P 		141.1
(-3)
P 		102
B 224.1
(2.5)
P 		134.3
(-3)
P 		184.1
(0.1)
P 		231.6
(3)
P 		97
C 240.2
(0.4)
P 		260.1
(1.7)
P 		280.8
(3)
P 		188
(-3)
P 		93
avr 235.8
(3) 		180.2
(-3) 		215.4
(0.8) 		186.9
(-2.3) 		56

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 130
1 2 3 4 5 6 range
A 37.7
(-3)
P 		154.8
(3)
P 		45.2
(-2.6)
P 		79.9
(-0.8)
P 		52.7
(-2.2)
P 		88.7
(-0.4)
P 		117
B 81.2
(1.5)
P 		66.8
(-1.7)
P 		63.4
(-2.4)
P 		60.7
(-3)
P 		88.2
(3)
P 		74.3
(-0)
P 		28
C 167.8
(3)
P 		57.7
(-2.4)
P 		78.6
(-1.4)
P 		45.2
(-3)
A 		95.9
(-0.5)
P 		59.8
(-2.3)
P 		123
avr 95.6
(3) 		93.1
(2.6) 		62.4
(-2.9) 		61.9
(-3) 		78.9
(0) 		74.3
(-0.8) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186328_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5352
Sequence Type Exemplar sequence
Representative Public ID F56D12.5 NCBI
Target Description F56D12.5 /REP_DB=WormBase Gene ID /WP=CE11254 /TR=O16646 /GB=AAB66119.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170974(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170974 NCBI Caenorhabditis elegans VIG (Drosophila Vasa Intronic Gene) ortholog family member (vig-1) (vig-1) mRNA, complete cds. 11/11 None
SNAP00000013796 ENSEMBL cdna:SNAP chromosome:CEL140:II:1311270:1322567:1 11/11 None
GENEFINDER00000013807 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1321154:1322567:1 11/11 None
F56D12.5a.2 ENSEMBL cdna:known chromosome:CEL140:II:1321041:1323206:1 gene:F56D12.5 11/11 None
F56D12.5a.1 ENSEMBL cdna:known chromosome:CEL140:II:1321043:1323206:1 gene:F56D12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173512 2 1 NM_170974 174822_at (11), 186328_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1321152-1322566(+) 100.0 100.0
Public Domain and Genome References
Ensembl F56D12.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB66120 Hypothetical protein F56D12.6b [Caenorhabditis elegans] ref|NP_493948.1| F56D12.6b [Caenorhabditis elegans] 0.0
blast AAD47126 Hypothetical protein F56D12.6a [Caenorhabditis elegans] ref|NP_493947.1| F56D12.6a [Caenorhabditis elegans] 0.0
blast CAE62915 Hypothetical protein CBG07108 [Caenorhabditis briggsae] 0.0
blast AAB66119 Vig (drosophila vasa intronic gene) ortholog protein 1, isoform a [Caenorhabditis elegans] ref|NP_740971.1| VIG (Drosophila Vasa Intronic Gene) ortholog family member (vig-1) [Caenorhabditis elegans] 8.0E-92
blast CAE62914 Hypothetical protein CBG07107 [Caenorhabditis briggsae] 1.0E-70
Protein Domains
Database ID Description E-Value
Pfam IPR006861 EMBL-EBI Hyaluronan/mRNA binding protein 2.9E-71
Pfam IPR006861 EMBL-EBI Hyaluronan/mRNA binding protein 2.9E-71
Sequence
Target Sequence 
>C. ELEGANS:186328_AT
acgtgggcgtcaattcgatcgtcaatccggatccgaccgtaccggagttcgctcattcga
caagaaggacggacacggaaagggcaactggggagaccagaaggacgagttggctggaga
aaccgagaacattgctccagagggcgctgagagcaccgagccagaggttccacgtgagaa
gaccgctgaggagctcgcctacgaggctgagttggctgttcttgccaagcaaaagactct
taaggagttcaaggctgcggctaaggctgacgctccaaagttcaacaccagaaaggccgg
agagggagccgccgatacttttggcaagctcgttccaatcaagaaggaggtcatcccaga
tcgtgaggaagacgaggtcgtcgtaatccacaaggctccaagaaagcaagtgctcgacat
ttcgatcactttccgcaacgatcgtccagaacgcgagcgcaacgaccgttctgagaggcc
gcaacgtggaggacca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGTGGGCGTCAATTCGATCGTCAA 510 93 537 Antisense
CAATCCGGATCCGACCGTACCGGAG 145 179 559 Antisense
AACATTGCTCCAGAGGGCGCTGAGA 274 137 664 Antisense
TGAGAGCACCGAGCCAGAGGTTCCA 242 569 684 Antisense
AGGAGCTCGCCTACGAGGCTGAGTT 91 57 725 Antisense
GGAGTTCAAGGCTGCGGCTAAGGCT 163 497 780 Antisense
GAGCCGCCGATACTTTTGGCAAGCT 93 387 842 Antisense
GAGGTCGTCGTAATCCACAAGGCTC 462 401 910 Antisense
GTGCTCGACATTTCGATCACTTTCC 431 479 946 Antisense
TCCGCAACGATCGTCCAGAACGCGA 83 597 968 Antisense
TGAGAGGCCGCAACGTGGAGGACCA 482 567 1008 Antisense
Sequence Source Affymetrix Proprietary Database