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[186316_s_at]

Description Y39E4A.2B /REP_DB=WormBase Gene ID /WP=CE16617 /TR=O45923 /GB=CAA16328.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 90      6grp_high1_10_10 > 19      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_highest_nonorm > 73      6grp_total > 01      4grp_total > 52      6grp_cgc > 99      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 243
1 2 3 4 range
A 588.3
(-1)
P 		723.3
(3)
P 		522.2
(-3)
P 		523.9
(-2.9)
P 		201
B 586.7
(0.9)
P 		566.7
(0.2)
P 		643
(3)
P 		481.1
(-3)
P 		162
C 588.5
(3)
P 		556.9
(1.3)
P 		480.1
(-3)
P 		481.1
(-2.9)
P 		108
avr 587.8
(1.6) 		615.6
(3) 		548.4
(-0.4) 		495.4
(-3) 		120

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 275
1 2 3 4 5 6 range
A 576
(0.8)
P 		621.6
(1.8)
P 		523.4
(-0.4)
P 		408.4
(-3)
P 		672.9
(3)
P 		540.8
(0)
P 		265
B 580.7
(-0.7)
P 		596.8
(-0.1)
P 		674.2
(2.7)
P 		605.8
(0.2)
P 		682.9
(3)
P 		517
(-3)
P 		166
C 532.5
(0.7)
P 		602.9
(3)
P 		416.8
(-3)
P 		439.6
(-2.3)
P 		547.9
(1.2)
P 		539.5
(1)
P 		186
avr 563.1
(0.1) 		607.1
(1.9) 		538.1
(-0.9) 		484.6
(-3) 		634.6
(3) 		532.4
(-1.1) 		150
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 138
1 2 3 4 range
A 214.2
(3)
P 		157
(0.5)
P 		136.5
(-0.4)
P 		76.7
(-3)
P 		138
B 187.1
(3)
P 		140
(-0.3)
P 		149
(0.3)
P 		102.5
(-3)
P 		85
C 194.8
(3)
P 		160.1
(1.2)
P 		167.3
(1.5)
P 		81.1
(-3)
P 		114
avr 198.7
(3) 		152.4
(0.5) 		150.9
(0.4) 		86.8
(-3) 		112

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 189
1 2 3 4 5 6 range
A 106.2
(-2.9)
P 		221.1
(3)
P 		114
(-2.5)
P 		103.6
(-3)
P 		203
(2.1)
P 		131.7
(-1.6)
P 		118
B 202.1
(-0.9)
P 		198.7
(-1)
P 		163.7
(-2.8)
P 		160.5
(-3)
P 		277.5
(3)
P 		192.8
(-1.3)
P 		117
C 197.4
(3)
P 		184
(2.3)
P 		106.1
(-2)
P 		88.4
(-3)
P 		177.3
(1.9)
P 		180.6
(2.1)
P 		109
avr 168.6
(0) 		201.3
(1.9) 		127.9
(-2.4) 		117.5
(-3) 		219.3
(3) 		168.4
(0) 		102
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186316_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6130
Sequence Type Exemplar sequence
Representative Public ID Y39E4A.2B NCBI
Target Description Y39E4A.2B /REP_DB=WormBase Gene ID /WP=CE16617 /TR=O45923 /GB=CAA16328.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067291(11), NM_067290(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067291 NCBI Caenorhabditis elegans Toxin-regulated Target of p38MAPK family member (ttm-1) (ttm-1) mRNA, complete cds. 11/11 A 
NM_067290 NCBI Caenorhabditis elegans Toxin-regulated Target of p38MAPK family member (ttm-1) (ttm-1) mRNA, complete cds. 11/11 A 
GENEFINDER00000009413 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:12952721:12956528:-1 11/11 None
SNAP00000009408 ENSEMBL cdna:SNAP chromosome:CEL140:III:12952746:12956528:-1 11/11 None
Y39E4A.2b ENSEMBL cdna:known chromosome:CEL140:III:12952497:12956528:-1 gene:Y39E4A.2 11/11 A 
Y39E4A.2a ENSEMBL cdna:known chromosome:CEL140:III:12952721:12966511:-1 gene:Y39E4A.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:12952716-12956524(-) 100.0 100.0
Public Domain and Genome References
Ensembl Y39E4A.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA16327 Hypothetical protein Y39E4A.2a [Caenorhabditis elegans] ref|NP_499691.1| Toxin-regulated Target of p38MAPK family member (ttm-1) [Caenorhabditis elegans] 1.0E-176
blast CAA16328 Hypothetical protein Y39E4A.2b [Caenorhabditis elegans] ref|NP_499692.1| Toxin-regulated Target of p38MAPK family member (ttm-1) [Caenorhabditis elegans] 1.0E-146
blast CAA16328 Hypothetical protein Y39E4A.2b [Caenorhabditis elegans] ref|NP_499692.1| Toxin-regulated Target of p38MAPK family member (ttm-1) [Caenorhabditis elegans] 1.0E-142
blast CAA16328 Hypothetical protein Y39E4A.2b [Caenorhabditis elegans] ref|NP_499692.1| Toxin-regulated Target of p38MAPK family member (ttm-1) [Caenorhabditis elegans] 1.0E-139
blast CAA16327 Hypothetical protein Y39E4A.2a [Caenorhabditis elegans] ref|NP_499691.1| Toxin-regulated Target of p38MAPK family member (ttm-1) [Caenorhabditis elegans] 1.0E-137
Protein Domains
Database ID Description E-Value
Pfam IPR002524 EMBL-EBI Cation efflux protein 1.7E-92
Pfam IPR002524 EMBL-EBI Cation efflux protein 1.7E-92
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499692.1 6 104-126,130-152,172-194,209-231,257-279,284-306
NP_499691.1 6 85-107,111-133,153-175,190-212,238-260,265-287
Y39E4A.2b 6 104-126,130-152,172-194,209-231,257-279,284-306
Y39E4A.2a 6 85-107,111-133,153-175,190-212,238-260,265-287
GENEFINDER00000009413 6 104-126,130-152,172-194,209-231,257-279,284-306
SNAP00000009408 6 104-126,130-152,172-194,209-231,257-279,284-306
Sequence
Target Sequence 
>C. ELEGANS:186316_S_AT
gagctgctctgattcatgtcattggagatttggtgcaatccattggagtcctgattgctg
cccttatcattaggtttaccggatggacacttgccgatccgatttgcacattccttttct
cgattatcgtgctcttcacgacggttacagttatgcgggacatctttttcgttttgatgg
aagccacaccgagccactatgacttgagtgatgtgaagaaggcgttgagtgcgttggaag
gtgtaaaaggtgttcatgatttgcatttatggtctattggaatggataagactgcgtttt
cggtgcatttggcacttgaatctcctaaccgcgccatggaaaatgttgccgaagcccgtt
ccctgatccgccggcgattcggagttgccgtcgccactgtccaagtggagccgttcgacg
a

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCTGCTCTGATTCATGTCATTGG 370 387 779 Antisense
TCCATTGGAGTCCTGATTGCTGCCC 440 591 817 Antisense
ACACTTGCCGATCCGATTTGCACAT 94 111 865 Antisense
ACATTCCTTTTCTCGATTATCGTGC 321 105 886 Antisense
GTGCTCTTCACGACGGTTACAGTTA 185 479 907 Antisense
ATGCGGGACATCTTTTTCGTTTTGA 42 41 931 Antisense
CACACCGAGCCACTATGACTTGAGT 258 187 963 Antisense
GACTGCGTTTTCGGTGCATTTGGCA 508 375 1068 Antisense
TTTGGCACTTGAATCTCCTAACCGC 2 667 1086 Antisense
CGCCGGCGATTCGGAGTTGCCGTCG 224 255 1147 Antisense
CTGTCCAAGTGGAGCCGTTCGACGA 239 213 1175 Antisense
Sequence Source Affymetrix Proprietary Database