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[186313_at]

Description Y49E10.18 /REP_DB=WormBase Gene ID /WP=CE22233 /TR=Q9XTU3 /GB=CAB11554.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 21      4grp_highest_8_8 > 33      4grp_high_8_8 > 58      6grp_highest_nonorm > 23      6grp_total > 32      4grp_total > 61      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2831
1 2 3 4 range
A 2955.9
(3)
P 		2326.6
(1.6)
P 		2483.4
(2)
P 		250.2
(-3)
P 		2706
B 2870.8
(3)
P 		2106.5
(1.3)
P 		2862.7
(3)
P 		124.8
(-3)
P 		2746
C 2855.4
(3)
P 		1844.5
(0.7)
P 		2477.6
(2.1)
P 		199.1
(-3)
P 		2656
avr 2894
(3) 		2092.5
(1.2) 		2607.9
(2.4) 		191.4
(-3) 		2703

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2235
1 2 3 4 5 6 range
A 2212.5
(1.4)
P 		2753.5
(3)
P 		2661.6
(2.7)
P 		2005.8
(0.8)
P 		1276.5
(-1.4)
P 		738.9
(-3)
P 		2015
B 1968.9
(1.2)
P 		2310.6
(2.4)
P 		2503.7
(3)
P 		1747.4
(0.5)
P 		1241.9
(-1.1)
P 		677.4
(-3)
P 		1826
C 2303.1
(1.4)
P 		2225.3
(1.2)
P 		2883.2
(3)
P 		1838.5
(0.2)
P 		1496.5
(-0.7)
P 		647.8
(-3)
P 		2235
avr 2161.5
(1.4) 		2429.8
(2.2) 		2682.8
(3) 		1863.9
(0.5) 		1338.3
(-1) 		688
(-3) 		1995
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1050
1 2 3 4 range
A 1076.3
(3)
P 		505.1
(-0.3)
P 		649.2
(0.5)
P 		36.6
(-3)
P 		1040
B 915.5
(3)
P 		520.2
(0.3)
P 		663.4
(1.3)
P 		26.6
(-3)
P 		889
C 945.2
(3)
P 		530.3
(0.3)
P 		863.5
(2.5)
P 		33.6
(-3)
P 		912
avr 979
(3) 		518.5
(0.1) 		725.4
(1.4) 		32.3
(-3) 		947

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 799
1 2 3 4 5 6 range
A 407.9
(-1.3)
P 		979.3
(3)
P 		579.6
(0)
P 		508.7
(-0.5)
P 		385.2
(-1.5)
P 		179.9
(-3)
P 		799
B 685.1
(2)
P 		769.1
(3)
P 		608
(1.1)
P 		462.8
(-0.6)
P 		504.5
(-0.1)
P 		252.6
(-3)
P 		517
C 853.7
(3)
P 		679
(1.4)
P 		733.8
(1.9)
P 		369.7
(-1.6)
P 		484.2
(-0.5)
P 		216.8
(-3)
P 		637
avr 648.9
(1.4) 		809.1
(3) 		640.5
(1.3) 		447.1
(-0.7) 		458
(-0.6) 		216.4
(-3) 		593
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186313_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7933
Sequence Type Exemplar sequence
Representative Public ID Y49E10.18 NCBI
Target Description Y49E10.18 /REP_DB=WormBase Gene ID /WP=CE22233 /TR=Q9XTU3 /GB=CAB11554.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067222(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067222 NCBI Caenorhabditis elegans Y49E10.18 (Y49E10.18) mRNA, complete cds. 11/11 None
SNAP00000016086 ENSEMBL cdna:SNAP chromosome:CEL140:III:12441136:12445976:-1 11/11 None
GENEFINDER00000016108 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:12442549:12444852:-1 11/11 None
Y49E10.18 ENSEMBL cdna:known chromosome:CEL140:III:12442515:12445994:-1 gene:Y49E10.18 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:12442544-12444848(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y49E10.18
Entrez Gene 176671 Entrez gene
SwissProt Q9XTU3 EMBL-EBI
WormBase CE33877 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6629 lipid metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
4806 triacylglycerol lipase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB11554 Hypothetical protein Y49E10.18 [Caenorhabditis elegans] ref|NP_499623.2| Y49E10.18 [Caenorhabditis elegans] 0.0
blast CAB61137 Hypothetical protein Y51H4A.5 [Caenorhabditis elegans] ref|NP_502962.1| Y51H4A.5 [Caenorhabditis elegans] 1.0E-147
blast CAB61137 Hypothetical protein Y51H4A.5 [Caenorhabditis elegans] ref|NP_502962.1| Y51H4A.5 [Caenorhabditis elegans] 1.0E-139
blast CAB11554 Hypothetical protein Y49E10.18 [Caenorhabditis elegans] ref|NP_499623.2| Y49E10.18 [Caenorhabditis elegans] 1.0E-139
blast CAB11552 Hypothetical protein Y49E10.16 [Caenorhabditis elegans] ref|NP_499621.1| Y49E10.16 [Caenorhabditis elegans] 1.0E-135
blast CAE66417 Hypothetical protein CBG11685 [Caenorhabditis briggsae] 1.0E-127
Protein Domains
Database ID Description E-Value
Pfam IPR002921 EMBL-EBI Lipase, class 3 3.3E-67
Pfam IPR002921 EMBL-EBI Lipase, class 3 3.3E-67
Pfam IPR002921 EMBL-EBI Lipase, class 3 3.3E-67
Sequence
Target Sequence 
>C. ELEGANS:186313_AT
aaaagtcgttgtcatgtcgtttcggggaactcaaggagccacacagttgactgaagagat
tttggacttttttacgggtaaaaagccatttttcaatgatgccggacacattttcaccta
cttctacgatgcattctttttcctctggaatggaggtcttcaacaagaaattcgtcgact
aaaataccaatatccagagtatgaattatgggttactgggcactcacttggtggagcaat
tgcatcgattgcagcttcttatgttgtccattcaggactttatactggtgatcaagtgaa
acttgttacgatgggacaaccgcgaaccggcgattatgattatgctgtttggcatgataa
gacttttccgtattcattccgaattgttcatcatagggatattgttccacacattcctcc
gcaatatggaaaggatgagcttttccatcacagaacagaagtttggtacaacaacaacat
gtcaagcacagacccatatcttatttgtgcggaagcagatggcctctactgtagtaatcg
acaattggactcttatccaccggatcacttaacctattt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAGTCGTTGTCATGTCGTTTCGG 69 133 102 Antisense
ATGATGCCGGACACATTTTCACCTA 35 43 197 Antisense
TCACCTACTTCTACGATGCATTCTT 53 627 215 Antisense
GTTACTGGGCACTCACTTGGTGGAG 111 445 313 Antisense
TTACGATGGGACAACCGCGAACCGG 113 639 407 Antisense
AGACTTTTCCGTATTCATTCCGAAT 397 67 461 Antisense
GGGATATTGTTCCACACATTCCTCC 563 495 497 Antisense
GTCAAGCACAGACCCATATCTTATT 57 465 582 Antisense
TGCGGAAGCAGATGGCCTCTACTGT 62 583 609 Antisense
GACAATTGGACTCTTATCCACCGGA 94 365 641 Antisense
ATCCACCGGATCACTTAACCTATTT 100 37 656 Antisense
Sequence Source Affymetrix Proprietary Database