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[186305_at]

Description H04M03.1 /REP_DB=WormBase Gene ID /WP=CE20920 /TR=Q9TYQ8 /GB=AAD12791.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 19      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 5.5
(-3)
A 		13.1
(-2.3)
A 		10.6
(-2.5)
A 		72.8
(3)
A 		67
B 49.1
(3)
A 		29.4
(-3)
A 		36.4
(-0.9)
A 		33.6
(-1.7)
A 		20
C 27.5
(-2)
A 		73.3
(1)
A 		12.1
(-3)
A 		103.6
(3)
P 		92
avr 27.4
(-2.1) 		38.6
(-0.7) 		19.7
(-3) 		70
(3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 125
1 2 3 4 5 6 range
A 37.7
(-1.6)
A 		23.6
(-2.3)
A 		21.6
(-2.4)
A 		9.4
(-3)
A 		62.2
(-0.4)
P 		133.1
(3)
P 		124
B 13.4
(-3)
A 		13.2
(-3)
A 		67
(0)
A 		69
(0.2)
A 		119.3
(3)
A 		92.3
(1.5)
P 		106
C 41.1
(-1.4)
A 		46.1
(-1.1)
A 		7.8
(-3)
A 		49.8
(-0.9)
A 		73.1
(0.2)
M 		129.3
(3)
P 		122
avr 30.7
(-2.8) 		27.6
(-3) 		32.1
(-2.7) 		42.7
(-2) 		84.9
(0.8) 		118.2
(3) 		91
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 2
(-3)
A 		2.9
(-2.4)
A 		2.8
(-2.4)
A 		10.7
(3)
A 		9
B 15.7
(3)
A 		7.3
(-2.9)
A 		8.4
(-2.2)
A 		7.2
(-3)
A 		9
C 9.1
(-1.3)
A 		21.1
(3)
A 		4.2
(-3)
A 		17.5
(1.7)
P 		17
avr 8.9
(0.4) 		10.4
(1.7) 		5.1
(-3) 		11.8
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 7
(-2.1)
A 		8.4
(-1.8)
A 		4.7
(-2.5)
A 		2.4
(-3)
A 		18.8
(0.3)
P 		32.4
(3)
P 		30
B 4.7
(-3)
A 		4.4
(-3)
A 		16.3
(-1.4)
A 		18.3
(-1.1)
A 		48.5
(3)
A 		34.4
(1.1)
P 		44
C 15.2
(-1.1)
A 		14.1
(-1.2)
A 		2
(-3)
A 		10
(-1.8)
A 		23.7
(0.2)
M 		43.3
(3)
P 		41
avr 9
(-2.7) 		9
(-2.7) 		7.7
(-3) 		10.2
(-2.5) 		30.3
(1.7) 		36.7
(3) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186305_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10585
Sequence Type Exemplar sequence
Representative Public ID H04M03.1 NCBI
Target Description H04M03.1 /REP_DB=WormBase Gene ID /WP=CE20920 /TR=Q9TYQ8 /GB=AAD12791.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068486(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068486 NCBI Caenorhabditis elegans H04M03.1 (H04M03.1) mRNA, complete cds. 11/11 None
SNAP00000034804 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5896672:5899037:1 11/11 None
GENEFINDER00000034808 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5896672:5899037:1 11/11 None
H04M03.1 ENSEMBL cdna:known chromosome:CEL140:IV:5896629:5899087:1 gene:H04M03.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5896677-5899043(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H04M03.1
Entrez Gene 177360 Entrez gene
SwissProt Q9TYQ8 EMBL-EBI
WormBase CE20920 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6094 gluconeogenesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4611 phosphoenolpyruvate carboxykinase activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAD12791 Hypothetical protein H04M03.1 [Caenorhabditis elegans] ref|NP_500887.1| H04M03.1 [Caenorhabditis elegans] 0.0
blast CAE62593 Hypothetical protein CBG06707 [Caenorhabditis briggsae] 0.0
blast CAE63747 Hypothetical protein CBG08279 [Caenorhabditis briggsae] 1.0E-50
Protein Domains
Database ID Description E-Value
Pfam IPR008209 EMBL-EBI Phosphoenolpyruvate carboxykinase (GTP) 0.0056
Pfam IPR008209 EMBL-EBI Phosphoenolpyruvate carboxykinase (GTP) 4.6E-16
Pfam IPR008209 EMBL-EBI Phosphoenolpyruvate carboxykinase (GTP) 1.8
Pfam IPR008209 EMBL-EBI Phosphoenolpyruvate carboxykinase (GTP) 6.9E-7
Pfam IPR008209 EMBL-EBI Phosphoenolpyruvate carboxykinase (GTP) 3.2E-6
Sequence
Target Sequence 
>C. ELEGANS:186305_AT
acacgaccagtatcctctgatcttagaggcaaatacttgggaagaaggtgtatgtgtggc
tgctggtattcgagtcagttctatgaagcaacaaaagatatccgaagaaagtgttaaaga
aagtgtagaaggagcttcaccacgtagaatccttgtggagtgcccgatgcttcgtgctga
tgccatcaactttagcattgctaaatacgtaaaacattggctggagatgggtgtgggagt
caagtcgtcatctgaaaatttcgagaatcctccgccaccgcaaatcttcttcaccaactt
gtaccaggaagttgatgggaaaccactttggcccggtggtgtcgataacgcaaggatttt
cgaatacatctatgaaagatgtgccaacccagccgatctctcaaagacaatctcttctgg
acttgggatagttccgaaaacacttcaattgagtgctggaaccaacttggcacctcttct
gcaagtggacattcgcttctggctcaccgagctcaataagctgcgtgcatttttcaatct
tc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACACGACCAGTATCCTCTGATCTTA 117 109 1281 Antisense
TGTGTGGCTGCTGGTATTCGAGTCA 350 555 1333 Antisense
AGAATCCTTGTGGAGTGCCCGATGC 650 75 1426 Antisense
GGGTGTGGGAGTCAAGTCGTCATCT 55 497 1509 Antisense
GAAAATTTCGAGAATCCTCCGCCAC 564 353 1534 Antisense
TGATGGGAAACCACTTTGGCCCGGT 60 565 1593 Antisense
GCCCGGTGGTGTCGATAACGCAAGG 57 287 1611 Antisense
ATGAAAGATGTGCCAACCCAGCCGA 132 45 1652 Antisense
GTGCTGGAACCAACTTGGCACCTCT 410 479 1733 Antisense
TGGCACCTCTTCTGCAAGTGGACAT 689 545 1748 Antisense
AAGCTGCGTGCATTTTTCAATCTTC 61 151 1798 Antisense
Sequence Source Affymetrix Proprietary Database