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[186299_at]

Description H20E11.2 /REP_DB=WormBase Gene ID /WP=CE19493 /TR=Q9TYZ0 /GB=AAC68974.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 51      4grp_highest_8_8 > 09      4grp_high_8_8 > 61      6grp_highest_nonorm > 80      6grp_total > 30      4grp_total > 58      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 61.9
(0.1)
P 		62.4
(0.2)
P 		73.3
(3)
P 		49.8
(-3)
P 		24
B 62.1
(-2.4)
P 		78.7
(-0.2)
P 		102.1
(3)
P 		57.8
(-3)
P 		44
C 75.3
(0.2)
P 		97.3
(3)
P 		74.1
(0.1)
P 		49.7
(-3)
P 		48
avr 66.4
(-0.3) 		79.5
(2.3) 		83.2
(3) 		52.4
(-3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 294
1 2 3 4 5 6 range
A 152.3
(-1)
P 		268.5
(2.7)
P 		276.8
(3)
P 		272.3
(2.9)
P 		195.7
(0.4)
P 		88.2
(-3)
P 		189
B 140.2
(-1.7)
P 		242.2
(0.4)
P 		370.7
(3)
P 		273.3
(1)
P 		175.4
(-1)
P 		78
(-3)
P 		293
C 171.6
(-0.4)
P 		236.3
(1.5)
P 		264.7
(2.2)
P 		291.7
(3)
P 		170.6
(-0.4)
P 		77.1
(-3)
P 		215
avr 154.7
(-1) 		249
(1.5) 		304.1
(3) 		279.1
(2.3) 		180.6
(-0.3) 		81.1
(-3) 		223
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 22.5
(3)
P 		13.5
(-0.6)
P 		19.2
(1.7)
P 		7.3
(-3)
P 		15
B 19.8
(0.9)
P 		19.4
(0.7)
P 		23.7
(3)
P 		12.3
(-3)
P 		11
C 24.9
(2.1)
P 		28
(3)
P 		25.8
(2.3)
P 		8.4
(-3)
P 		20
avr 22.4
(2.8) 		20.3
(1.9) 		22.9
(3) 		9.3
(-3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 74
1 2 3 4 5 6 range
A 28.1
(-2.5)
P 		95.5
(3)
P 		60.3
(0.1)
P 		69.1
(0.9)
P 		59.1
(0)
P 		21.5
(-3)
P 		74
B 48.8
(-1.1)
P 		80.6
(2.1)
P 		90
(3)
P 		72.4
(1.3)
P 		71.3
(1.2)
P 		29.1
(-3)
P 		61
C 63.6
(1.9)
P 		72.1
(3)
P 		67.4
(2.4)
P 		58.7
(1.3)
P 		55.2
(0.8)
P 		25.8
(-3)
P 		46
avr 46.8
(-0.8) 		82.7
(3) 		72.6
(1.9) 		66.7
(1.3) 		61.9
(0.8) 		25.5
(-3) 		57
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186299_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9531
Sequence Type Exemplar sequence
Representative Public ID H20E11.2 NCBI
Target Description H20E11.2 /REP_DB=WormBase Gene ID /WP=CE19493 /TR=Q9TYZ0 /GB=AAC68974.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068650(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068650 NCBI Caenorhabditis elegans H20E11.2 (H20E11.2) mRNA, complete cds. 11/11 None
SNAP00000015309 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6827151:6829474:1 11/11 None
GENEFINDER00000015312 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6824790:6829474:1 11/11 None
H20E11.2 ENSEMBL cdna:known chromosome:CEL140:IV:6827761:6829474:1 gene:H20E11.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6827766-6829480(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H20E11.2
Entrez Gene 186754 Entrez gene
SwissProt Q9TYZ0 EMBL-EBI
WormBase CE19493 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68974 Hypothetical protein H20E11.2 [Caenorhabditis elegans] ref|NP_501051.1| H20E11.2 [Caenorhabditis elegans] 0.0
blast AAP86616 Hypothetical protein H20E11.3b [Caenorhabditis elegans] ref|NP_001023262.1| H20E11.3b [Caenorhabditis elegans] 0.0
blast AAC68975 Hypothetical protein H20E11.3a [Caenorhabditis elegans] ref|NP_001023261.1| H20E11.3a [Caenorhabditis elegans] 1.0E-159
blast AAP86616 Hypothetical protein H20E11.3b [Caenorhabditis elegans] ref|NP_001023262.1| H20E11.3b [Caenorhabditis elegans] 1.0E-155
blast AAP86616 Hypothetical protein H20E11.3b [Caenorhabditis elegans] ref|NP_001023262.1| H20E11.3b [Caenorhabditis elegans] 1.0E-144
blast AAC68975 Hypothetical protein H20E11.3a [Caenorhabditis elegans] ref|NP_001023261.1| H20E11.3a [Caenorhabditis elegans] 1.0E-109
Protein Domains
Database ID Description E-Value
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 2.0E-12
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 9.5E-11
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 7.4E-16
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 7.2E-10
Sequence
Target Sequence 
>C. ELEGANS:186299_AT
taggggttctgatccaaacttttcaaattccttgtcagttgtcggaacagagcaaatttc
agtaagtttggctacatctcgttaccaatatccaactgatttgctctataacacatttgt
aattgatggagatttctataatcaagcatcagttcacagactattaccacttcaaggagg
tccacctttacaaacaatcggaaataaagtgactatcgtgacatttgtcaatgagacatc
tactgaatgtgcagttgttttaaatccaaaatcagaagctgatggttttgattatttatc
atctcaagcaagtattaatggagaaattaataaaatcagttttgctccattggatcagag
acaaggtctacaagttattgctcttcataatactcaaatcattatggattcgttggtact
tgatactaaaactgattttttctgcactgcaatagtaatatctggaccaccaaataattc
ttctcatctacttcttaatttggaaaaagctcaaggacttatgccatacacttttaatat
gtcatacttcactgttgttgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAGGGGTTCTGATCCAAACTTTTCA 501 605 495 Antisense
GGCTACATCTCGTTACCAATATCCA 674 537 564 Antisense
CCAACTGATTTGCTCTATAACACAT 104 277 586 Antisense
GTTCACAGACTATTACCACTTCAAG 440 445 646 Antisense
CACTTCAAGGAGGTCCACCTTTACA 576 99 662 Antisense
AATCAGTTTTGCTCCATTGGATCAG 359 167 828 Antisense
AGGTCTACAAGTTATTGCTCTTCAT 18 59 858 Antisense
ACTGATTTTTTCTGCACTGCAATAG 301 97 925 Antisense
GGACCACCAAATAATTCTTCTCATC 291 523 958 Antisense
AGCTCAAGGACTTATGCCATACACT 3 81 1002 Antisense
ATATGTCATACTTCACTGTTGTTGG 447 21 1031 Antisense
Sequence Source Affymetrix Proprietary Database