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[186286_at]

Description Y27F2A.6 /REP_DB=WormBase Gene ID /WP=CE21493 /TR=Q9N548 /GB=AAF60473.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 70      4grp_total > 02      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 3.6
(0.8)
A 		4.4
(2.3)
A 		1.6
(-3)
A 		4.8
(3)
A 		3
B 0.9
(-3)
A 		1.3
(-2.4)
A 		1.2
(-2.6)
A 		5.2
(3)
A 		4
C 3.7
(-2.8)
A 		1.8
(-3)
A 		2.1
(-3)
A 		50.1
(3)
A 		48
avr 2.7
(-2.6) 		2.5
(-2.7) 		1.6
(-3) 		20
(3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 6.3
(-0.7)
A 		1.7
(-3)
A 		3.4
(-2.2)
A 		13.7
(3)
A 		9.4
(0.9)
A 		5
(-1.4)
A 		12
B 1.1
(-3)
A 		2.5
(-2.3)
A 		7.7
(0.2)
A 		13.5
(3)
A 		4.3
(-1.5)
A 		8.1
(0.4)
A 		12
C 0.9
(-3)
A 		2.6
(-2)
A 		7.3
(0.7)
A 		11.4
(3)
A 		0.9
(-3)
A 		2.2
(-2.3)
A 		11
avr 2.8
(-2.7) 		2.3
(-3) 		6.1
(-0.8) 		12.9
(3) 		4.9
(-1.5) 		5.1
(-1.4) 		11
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 1.3
(3)
A 		1
(1)
A 		0.4
(-3)
A 		0.7
(-1)
A 		1
B 0.3
(-3)
A 		0.3
(-3)
A 		0.3
(-3)
A 		1.1
(3)
A 		1
C 1.2
(-2.5)
A 		0.5
(-3)
A 		0.7
(-2.8)
A 		8.4
(3)
A 		8
avr 0.9
(-2.2) 		0.6
(-2.8) 		0.5
(-3) 		3.4
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3
1 2 3 4 5 6 range
A 1.2
(-1.8)
A 		0.6
(-3)
A 		0.7
(-2.8)
A 		3.5
(3)
A 		2.8
(1.6)
A 		1.2
(-1.8)
A 		3
B 0.4
(-3)
A 		0.8
(-2.3)
A 		1.9
(-0.2)
A 		3.6
(3)
A 		1.7
(-0.6)
A 		3
(1.9)
A 		3
C 0.3
(-3)
A 		0.8
(-1.5)
A 		1.9
(1.8)
A 		2.3
(3)
A 		0.3
(-3)
A 		0.7
(-1.8)
A 		2
avr 0.6
(-3) 		0.7
(-2.8) 		1.5
(-0.8) 		3.1
(3) 		1.6
(-0.6) 		1.6
(-0.6) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186286_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4695
Sequence Type Exemplar sequence
Representative Public ID Y27F2A.6 NCBI
Target Description Y27F2A.6 /REP_DB=WormBase Gene ID /WP=CE21493 /TR=Q9N548 /GB=AAF60473.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062065(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062065 NCBI Caenorhabditis elegans Y27F2A.6 (Y27F2A.6) mRNA, complete cds. 11/11 A 
SNAP00000006766 ENSEMBL cdna:SNAP chromosome:CEL140:II:3174285:3181124:-1 9/11 None
GENEFINDER00000006778 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3180535:3182683:-1 11/11 None
Y27F2A.6 ENSEMBL cdna:known chromosome:CEL140:II:3180535:3182671:-1 gene:Y27F2A.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2675799-2676865(+) 56.56 56.56
chrII:3180533-3182670(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y27F2A.6
Entrez Gene 189560 Entrez gene
SwissProt Q9N548 EMBL-EBI
WormBase CE21493 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494466 Y27F2A.6 [Caenorhabditis elegans] gb|AAF60473.1| Hypothetical protein Y27F2A.6 [Caenorhabditis elegans] 0.0
blast NP_494329 F22E5.1 [Caenorhabditis elegans] gb|AAB66167.1| Hypothetical protein F22E5.1 [Caenorhabditis elegans] 1.0E-158
blast NP_494329 F22E5.1 [Caenorhabditis elegans] gb|AAB66167.1| Hypothetical protein F22E5.1 [Caenorhabditis elegans] 1.0E-156
blast AAB18283 Hypothetical protein C16D9.5 [Caenorhabditis elegans] ref|NP_505125.2| C16D9.5 [Caenorhabditis elegans] 1.0E-96
blast AAB18283 Hypothetical protein C16D9.5 [Caenorhabditis elegans] ref|NP_505125.2| C16D9.5 [Caenorhabditis elegans] 2.0E-95
blast NP_494464 Y27F2A.5 [Caenorhabditis elegans] gb|AAF60476.2| Hypothetical protein Y27F2A.5 [Caenorhabditis elegans] 2.0E-83
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494466.1 1 5-22
Y27F2A.6 1 5-22
GENEFINDER00000006778 1 7-26
Sequence
Target Sequence 
>C. ELEGANS:186286_AT
gcaaaaacggttccgagcggcgctgaaaattcgataaaaggagtgaataatgcgacttca
aaaatgcgtgccagcgtcgatgaaattatgaagaaccgtgtggaaaaacagaaattagcc
gaagaatctgaaatggcaaagctgaaaaagtctctggaaggcgtctctcaagcaaattcc
gacttaaaaatgaagaacttgaatcttttaatcgagacgtcagatttgaaatcaaaatcg
gagcacacaatttcgacaagcgaacatcagaaaattgtctcaaaattacacgccgaaatc
gaaaatctgacaaaatccaaagaatcgcgctttgaatctctcaaaaccatattcagtttc
ccgaaacgagatgtggaacctggaaactctgttcaaaaatctgtgtcgaaattggattta
ttcaagagatgtttcgagattttcagtgcttacgcgatagccgaccgcctcgccaaaaca
ctaaaaaagggcatcttcctcagtccattggtcgtcattgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAAAAACGGTTCCGAGCGGCGCTG 32 319 922 Antisense
AAATGCGTGCCAGCGTCGATGAAAT 347 119 983 Antisense
AAAAAGTCTCTGGAAGGCGTCTCTC 93 129 1066 Antisense
GGCGTCTCTCAAGCAAATTCCGACT 320 541 1081 Antisense
CGGAGCACACAATTTCGACAAGCGA 588 243 1160 Antisense
AAGAATCGCGCTTTGAATCTCTCAA 85 153 1241 Antisense
GAGATGTGGAACCTGGAAACTCTGT 659 395 1289 Antisense
GAGATGTTTCGAGATTTTCAGTGCT 173 397 1347 Antisense
TTCAGTGCTTACGCGATAGCCGACC 423 685 1363 Antisense
AAAAAAGGGCATCTTCCTCAGTCCA 644 127 1404 Antisense
TCCTCAGTCCATTGGTCGTCATTGG 38 591 1418 Antisense
Sequence Source Affymetrix Proprietary Database