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[186283_at]

Description F32E10.1 /REP_DB=WormBase Gene ID /WP=CE04474 /TR=Q19974 /GB=AAA83359.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 76      4grp_highest_8_8 > 41      4grp_high_8_8 > 49      6grp_highest_nonorm > 82      6grp_total > 66      4grp_total > 62      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_rule_based > S6      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 195
1 2 3 4 range
A 366.9
(3)
P 		329.6
(0.9)
P 		261.4
(-3)
P 		266.9
(-2.7)
P 		106
B 333.2
(3)
P 		309.4
(1.6)
P 		324.3
(2.5)
P 		231.7
(-3)
P 		102
C 422.7
(3)
P 		296.2
(-0.9)
P 		329.1
(0.1)
P 		228.1
(-3)
P 		195
avr 374.3
(3) 		311.7
(0.2) 		304.9
(-0.2) 		242.2
(-3) 		132

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 232
1 2 3 4 5 6 range
A 249.5
(-3)
P 		324.6
(0.1)
P 		332.9
(0.4)
P 		346.3
(1)
P 		367.2
(1.8)
P 		395.4
(3)
P 		146
B 331.1
(-1.7)
P 		352.4
(-1)
P 		407.8
(0.7)
P 		347.4
(-1.2)
P 		288.4
(-3)
P 		481.8
(3)
P 		193
C 366.3
(0.2)
P 		308.7
(-1.8)
P 		274.6
(-3)
P 		317.3
(-1.5)
P 		317
(-1.5)
P 		443.9
(3)
P 		169
avr 315.6
(-3) 		328.6
(-2.4) 		338.4
(-1.9) 		337
(-2) 		324.2
(-2.6) 		440.4
(3) 		125
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 133.6
(3)
P 		71.5
(-0.9)
P 		68.3
(-1.1)
P 		39.1
(-3)
P 		95
B 106.3
(3)
P 		76.4
(-0.2)
P 		75.2
(-0.3)
P 		49.4
(-3)
P 		57
C 139.9
(3)
P 		85.2
(-0.2)
P 		114.7
(1.5)
P 		38.5
(-3)
P 		101
avr 126.6
(3) 		77.7
(-0.5) 		86.1
(0.1) 		42.3
(-3) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 134
1 2 3 4 5 6 range
A 46
(-3)
P 		115.5
(3)
P 		72.5
(-0.7)
P 		87.8
(0.6)
P 		110.8
(2.6)
P 		96.3
(1.3)
P 		70
B 115.2
(-1.4)
P 		117.3
(-1.3)
P 		99
(-2.5)
P 		92
(-3)
P 		117.2
(-1.3)
P 		179.7
(3)
P 		88
C 135.8
(2.1)
P 		94.2
(-0.8)
P 		69.9
(-2.6)
P 		63.8
(-3)
P 		102.6
(-0.3)
P 		148.6
(3)
P 		85
avr 99
(-1.2) 		109
(-0.2) 		80.5
(-3) 		81.2
(-2.9) 		110.2
(-0.1) 		141.5
(3) 		61
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186283_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9242
Sequence Type Exemplar sequence
Representative Public ID F32E10.1 NCBI
Target Description F32E10.1 /REP_DB=WormBase Gene ID /WP=CE04474 /TR=Q19974 /GB=AAA83359.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068832(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068832 NCBI Caenorhabditis elegans F32E10.1 (F32E10.1) mRNA, complete cds. 11/11 None
SNAP00000038469 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7580588:7583495:1 11/11 None
GENEFINDER00000038477 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7580588:7583495:1 11/11 None
F32E10.1 ENSEMBL cdna:known chromosome:CEL140:IV:7580581:7583705:1 gene:F32E10.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7580593-7583501(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F32E10.1
Entrez Gene 177538 Entrez gene
SwissProt Q19974 EMBL-EBI
WormBase CE04474 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251678_AT glycine-rich protein at
CANINE:1598936_AT similar to CG7516-PA cfa
CANINE_2:CFA.18485.1.S1_AT similar to CG7516-PA cfa
CANINE_2:CFAAFFX.6112.1.S1_S_AT similar to CG7516-PA cfa
CANINE_2:CFAAFFX.6116.1.S1_AT similar to CG7516-PA cfa
DROSOPHILA_2:1637468_AT dm
DROSGENOME1:154953_AT dm
CHICKEN:GGA.10415.1.S1_A_AT nucleolar protein 10 gga
CHICKEN:GGA.10415.1.S1_AT nucleolar protein 10 gga
CHICKEN:GGA.10415.2.S1_A_AT nucleolar protein 10 gga
HG-U133_PLUS_2:218591_S_AT nucleolar protein 10 hs
HG-U133A:218591_S_AT nucleolar protein 10 hs
HG-U133A_2:218591_S_AT nucleolar protein 10 hs
HU35KSUBA:RC_AA609616_AT nucleolar protein 10 hs
U133_X3P:G13430871_3P_A_AT nucleolar protein 10 hs
U133_X3P:HS.235498.0.S1_3P_A_AT nucleolar protein 10 hs
HG-U133_PLUS_2:222684_S_AT nucleolar protein 10 hs
HG-U133B:222684_S_AT nucleolar protein 10 hs
HG-U95B:52261_AT nucleolar protein 10 hs
HG-U95B:56271_AT nucleolar protein 10 hs
HG-U133_PLUS_2:1560082_AT Nucleolar protein 10 hs
HG-U95D:72697_AT Nucleolar protein 10 hs
MG-U74BV2:107605_AT nucleolar protein 10 mm
MG-U74BV2:162685_R_AT nucleolar protein 10 mm
MOE430B:1433739_AT nucleolar protein 10 mm
MOUSE430_2:1433739_AT nucleolar protein 10 mm
MOE430B:1457591_AT Nucleolar protein 10 (Nol10), mRNA mm
MOUSE430_2:1457591_AT Nucleolar protein 10 (Nol10), mRNA mm
MU19KSUBC:TC41137_AT Nucleolar protein 10 (Nol10), mRNA mm
MU19KSUBC:TC41137_G_AT Nucleolar protein 10 (Nol10), mRNA mm
RAE230B:1380201_AT similar to hypothetical protein FLJ14075 rn
RAT230_2:1380201_AT similar to hypothetical protein FLJ14075 rn
YEAST_2:1773994_AT Essential nucleolar protein of unknown function; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Sc
Protein Similarities
Method ID Description E-Value
blast AAA83359 Hypothetical protein F32E10.1 [Caenorhabditis elegans] ref|NP_501233.1| F32E10.1 [Caenorhabditis elegans] 0.0
blast CAE61922 Hypothetical protein CBG05918 [Caenorhabditis briggsae] 0.0
blast NP_998842 nucleolar protein 10 [Xenopus tropicalis] gb|AAH67979.1| Nucleolar protein 10 [Xenopus tropicalis] sp|Q6NVM6|NOL10_XENTR Nucleolar protein 1.0E-142
Sequence
Target Sequence 
>C. ELEGANS:186283_AT
caaaacgagccaaagttcgcgccgctaaaaaatctgaacgaaacattcaaaaatcgcaga
aatacgacgaacggaaagaggcgaagaagattaggaaagaacagcgacagttatcgaaac
cttccaaattcgtacttcattcaattggaaccggcgagacgactagaaagtttgttgacg
aaactgtcgcggtcagagatgatgctgacaacaaggactcgatggctctcggtgcccgta
aaagagcaactgcgagaagtgatagaaagactaagaaggtcaacgatgatgatgtaccct
tcggaggtcgcgagatgacattcacgatttcgaaacgtgataaggatcttcgtgcagaag
ctgcaaagacgaaacaagcagctcatgcagctgaacgaaaagaagtgagccgacgcccga
atgctctggtcacaaaagga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAAACGAGCCAAAGTTCGCGCCGC 342 191 1784 Antisense
GCGACAGTTATCGAAACCTTCCAAA 308 287 1887 Antisense
ACCTTCCAAATTCGTACTTCATTCA 574 81 1902 Antisense
ATTGGAACCGGCGAGACGACTAGAA 45 3 1927 Antisense
GACGAAACTGTCGCGGTCAGAGATG 438 377 1960 Antisense
GACAACAAGGACTCGATGGCTCTCG 527 365 1990 Antisense
TGATGATGTACCCTTCGGAGGTCGC 271 565 2070 Antisense
GAGGTCGCGAGATGACATTCACGAT 337 403 2087 Antisense
GACGAAACAAGCAGCTCATGCAGCT 16 381 2151 Antisense
CGAAAAGAAGTGAGCCGACGCCCGA 112 251 2179 Antisense
CCCGAATGCTCTGGTCACAAAAGGA 298 261 2199 Antisense
Sequence Source Affymetrix Proprietary Database