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[186280_s_at]

Description F33G12.3 /REP_DB=WormBase Gene ID /WP=CE04481 /TR=Q19987 /GB=AAK31509.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 86      4grp_highest_8_8 > 32      4grp_high_8_8 > 51      6grp_highest_nonorm > 92      6grp_total > 77      4grp_total > 52      6grp_highest_sub > Da      6grp_high1_sub > In      6grp_total_sub > Increase      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 146
1 2 3 4 range
A 218.8
(2.6)
P 		223
(3)
P 		180.6
(-1.3)
P 		163.3
(-3)
P 		60
B 249.3
(1.8)
P 		256.4
(2.3)
P 		268.4
(3)
P 		170.9
(-3)
P 		98
C 251.3
(3)
P 		238.7
(2.4)
P 		207.5
(1)
P 		122.4
(-3)
P 		129
avr 239.8
(3) 		239.4
(3) 		218.8
(1.6) 		152.2
(-3) 		88

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 265
1 2 3 4 5 6 range
A 167
(-3)
P 		288.9
(0)
P 		331.5
(1.1)
P 		218
(-1.7)
P 		301.1
(0.3)
P 		408.6
(3)
P 		242
B 269.8
(-0.9)
P 		181.4
(-3)
P 		281.7
(-0.6)
P 		270.8
(-0.9)
P 		304.6
(-0.1)
P 		432
(3)
P 		251
C 275.3
(-0.8)
P 		213
(-2.5)
P 		210.9
(-2.5)
P 		193.2
(-3)
P 		312.9
(0.2)
P 		420.7
(3)
P 		228
avr 237.4
(-2.7) 		227.8
(-3) 		274.7
(-1.5) 		227.3
(-3) 		306.2
(-0.5) 		420.4
(3) 		193
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 79.7
(3)
P 		48.4
(-0.4)
P 		47.2
(-0.5)
P 		23.9
(-3)
P 		56
B 79.5
(3)
P 		63.3
(0.7)
P 		62.2
(0.6)
P 		36.4
(-3)
P 		43
C 83.2
(3)
P 		68.6
(1.6)
P 		72.3
(2)
P 		20.6
(-3)
P 		63
avr 80.8
(3) 		60.1
(0.7) 		60.6
(0.7) 		27
(-3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 130
1 2 3 4 5 6 range
A 30.8
(-3)
P 		102.7
(3)
P 		72.2
(0.5)
P 		55.3
(-1)
P 		90.8
(2)
P 		99.5
(2.7)
P 		72
B 93.9
(-1)
P 		60.4
(-3)
P 		68.4
(-2.5)
P 		71.7
(-2.3)
P 		123.8
(0.8)
P 		161.1
(3)
P 		101
C 102
(0.7)
P 		65
(-1.5)
P 		53.7
(-2.1)
P 		38.9
(-3)
P 		101.3
(0.7)
P 		140.8
(3)
P 		102
avr 75.6
(-1.4) 		76
(-1.4) 		64.8
(-2.3) 		55.3
(-3) 		105.3
(0.8) 		133.8
(3) 		79
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186280_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5258
Sequence Type Exemplar sequence
Representative Public ID F33G12.3 NCBI
Target Description F33G12.3 /REP_DB=WormBase Gene ID /WP=CE04481 /TR=Q19987 /GB=AAK31509.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062526(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062526 NCBI Caenorhabditis elegans F33G12.3 (F33G12.3) mRNA, complete cds. 11/11 None
SNAP00000025574 ENSEMBL cdna:SNAP chromosome:CEL140:II:5031976:5034012:-1 11/11 None
GENEFINDER00000025579 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5031976:5034012:-1 11/11 None
F33G12.3 ENSEMBL cdna:known chromosome:CEL140:II:5031862:5034019:-1 gene:F33G12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5031973-5034010(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F33G12.3
Entrez Gene 173868 Entrez gene
SwissProt Q19987 EMBL-EBI
WormBase CE04481 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:245733_AT expressed protein at
CANINE:1604902_AT similar to expressed sequence AW209491 cfa
CANINE_2:CFA.2582.1.S1_AT similar to expressed sequence AW209491 cfa
CANINE_2:CFAAFFX.576.1.S1_AT similar to expressed sequence AW209491 cfa
DROSOPHILA_2:1634518_AT dm
DROSGENOME1:147941_AT dm
CHICKEN:GGA.10610.1.S1_S_AT similar to Chromosome 7 open reading frame 25 gga
CHICKEN:GGA.11392.1.S1_AT similar to Chromosome 7 open reading frame 25 gga
HG-U133_PLUS_2:221573_AT chromosome 7 open reading frame 25 hs
HG-U133A_2:221573_AT chromosome 7 open reading frame 25 hs
HG-U133A:221573_AT chromosome 7 open reading frame 25 hs
HU35KSUBC:RC_AA398392_AT chromosome 7 open reading frame 25 hs
U133_X3P:G12653950_3P_S_AT chromosome 7 open reading frame 25 hs
U133_X3P:4873825C_3P_AT chromosome 7 open reading frame 25 hs
HG-U133A_2:53202_AT chromosome 7 open reading frame 25 hs
HG-U133_PLUS_2:53202_AT chromosome 7 open reading frame 25 hs
HG-U133A:53202_AT chromosome 7 open reading frame 25 hs
HG-U95B:53202_AT chromosome 7 open reading frame 25 hs
HG-U95D:71508_AT Chromosome 7 open reading frame 25 hs
MG-U74BV2:113133_AT expressed sequence AW209491 mm
MOE430A:1423512_AT expressed sequence AW209491 mm
MOUSE430A_2:1423512_AT expressed sequence AW209491 mm
MOUSE430_2:1423512_AT expressed sequence AW209491 mm
MU19KSUBA:TC14725_AT Expressed sequence AW209491, mRNA (cDNA clone MGC:38935 IMAGE:5362794) mm
RG-U34C:RC_AI233244_AT similar to expressed sequence AW209491 (predicted) rn
RAE230A:1373547_AT similar to expressed sequence AW209491 (predicted) rn
RAT230_2:1373547_AT similar to expressed sequence AW209491 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4197 cysteine-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK31509 Hypothetical protein F33G12.3 [Caenorhabditis elegans] ref|NP_494927.1| F33G12.3 [Caenorhabditis elegans] 0.0
blast CAE59024 Hypothetical protein CBG02301 [Caenorhabditis briggsae] 1.0E-149
blast CAE59024 Hypothetical protein CBG02301 [Caenorhabditis briggsae] 1.0E-143
blast XP_418867 PREDICTED: similar to Chromosome 7 open reading frame 25 [Gallus gallus] 1.0E-28
Protein Domains
Database ID Description E-Value
scop a.2.5.Prefoldin All alpha proteins; Long alpha-hairpin; Prefoldin; Prefoldin 0.349999994039536
scop a.2.5.Prefoldin All alpha proteins; Long alpha-hairpin; Prefoldin; Prefoldin 0.219999998807907
Pfam IPR010733 EMBL-EBI Protein of unknown function DUF1308 1.0E-126
Pfam IPR010733 EMBL-EBI Protein of unknown function DUF1308 1.9E-69
Pfam IPR010733 EMBL-EBI Protein of unknown function DUF1308 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:186280_S_AT
gatgaagatgctgcttctattccaacgctaccctcctcacaatcgcagtcagagtcaaca
ctgccacgcttaaatcttgatatttcggctgtgatgttgttagtttcgaacatgtgtgaa
cctggtggtgtgaattatcaattcaaagaggaaatgatcaataagcatgtggaatgtgaa
cgattgaaacctgcgaaggctgaagtgctggaaaaattgaaaggacatcatctgattatg
tgtgagcttgcctatagaagagtagcagaaatcgtaaaaaatgtcggcgggcctacggag
aagaagcgattcgaagagctaagcaagacgattgagcgtgttcaggatcaaccatcagat
gaaattggaagagttgtcaaattacgaaatatcaaaggaataacgtcaattacaaagaga
gtcttctcatgcgcggaagttaccaaaacgactactgtaactgccaatcaaaagtttttg
acacattctagggctaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGAAGATGCTGCTTCTATTCCAA 133 413 649 Antisense
CCTCCTCACAATCGCAGTCAGAGTC 282 267 680 Antisense
GAGTCAACACTGCCACGCTTAAATC 594 399 700 Antisense
TCGAACATGTGTGAACCTGGTGGTG 544 597 754 Antisense
AACCTGCGAAGGCTGAAGTGCTGGA 82 145 836 Antisense
ATCTGATTATGTGTGAGCTTGCCTA 173 35 878 Antisense
AAATGTCGGCGGGCCTACGGAGAAG 110 121 927 Antisense
GAGCGTGTTCAGGATCAACCATCAG 317 387 982 Antisense
GAGAGTCTTCTCATGCGCGGAAGTT 440 395 1065 Antisense
GACTACTGTAACTGCCAATCAAAAG 129 373 1098 Antisense
GTTTTTGACACATTCTAGGGCTAAG 246 449 1122 Antisense
Sequence Source Affymetrix Proprietary Database