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[186270_s_at]

Description F53C3.12 /REP_DB=WormBase Gene ID /WP=CE19450 /TR=Q9TXT9 /GB=AAC67462.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 24      6grp_high1_10_10 > 41      4grp_highest_8_8 > 63      4grp_high_8_8 > 27      6grp_highest_nonorm > 62      6grp_total > 31      4grp_total > 37      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 165
1 2 3 4 range
A 464.4
(3)
P 		300.7
(-3)
P 		361.4
(-0.8)
P 		330.7
(-1.9)
P 		164
B 307.3
(-2.5)
P 		402.6
(3)
P 		299.2
(-3)
P 		392.8
(2.4)
P 		103
C 397
(1.6)
P 		423.1
(3)
P 		312.3
(-3)
P 		388.5
(1.1)
P 		111
avr 389.6
(3) 		375.5
(1.7) 		324.3
(-3) 		370.7
(1.3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 518
1 2 3 4 5 6 range
A 345.6
(-1.9)
P 		583.8
(3)
P 		521.8
(1.7)
P 		476.5
(0.8)
P 		344.6
(-2)
P 		294.3
(-3)
P 		290
B 496.3
(0)
P 		593.1
(1.3)
P 		717.3
(3)
P 		372.2
(-1.6)
P 		311.1
(-2.4)
P 		269.7
(-3)
P 		448
C 532
(0.4)
P 		564
(0.8)
P 		758.2
(3)
P 		574.5
(0.9)
P 		422.5
(-0.9)
P 		239.8
(-3)
P 		518
avr 458
(-0.1) 		580.3
(1.7) 		665.8
(3) 		474.4
(0.1) 		359.4
(-1.6) 		267.9
(-3) 		398
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 121
1 2 3 4 range
A 169.1
(3)
P 		65.3
(-2.2)
P 		94.5
(-0.7)
P 		48.4
(-3)
P 		121
B 98
(2.7)
P 		99.4
(3)
P 		69.3
(-3)
P 		83.7
(-0.1)
P 		30
C 131.4
(3)
P 		121.6
(2.1)
P 		108.8
(0.9)
P 		65.5
(-3)
P 		66
avr 132.8
(3) 		95.4
(-0.4) 		90.9
(-0.8) 		65.9
(-3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 144
1 2 3 4 5 6 range
A 63.7
(-3)
P 		207.6
(3)
P 		113.6
(-0.9)
P 		120.8
(-0.6)
P 		104
(-1.3)
P 		71.6
(-2.7)
P 		144
B 172.7
(1.5)
P 		197.4
(3)
P 		174.2
(1.6)
P 		98.6
(-3)
P 		126.4
(-1.3)
P 		100.6
(-2.9)
P 		99
C 197.2
(3)
P 		172.1
(1.7)
P 		193
(2.8)
P 		115.5
(-1.2)
P 		136.7
(-0.1)
P 		80.3
(-3)
P 		117
avr 144.5
(0.3) 		192.4
(3) 		160.3
(1.2) 		111.6
(-1.5) 		122.4
(-0.9) 		84.2
(-3) 		108
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186270_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4729
Sequence Type Exemplar sequence
Representative Public ID F53C3.12 NCBI
Target Description F53C3.12 /REP_DB=WormBase Gene ID /WP=CE19450 /TR=Q9TXT9 /GB=AAC67462.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062293(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062293 NCBI Caenorhabditis elegans F53C3.12 (F53C3.12) mRNA, complete cds. 11/11 None
SNAP00000027227 ENSEMBL cdna:SNAP chromosome:CEL140:II:3908537:3910825:-1 11/11 None
GENEFINDER00000027234 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3908537:3910825:-1 11/11 None
F53C3.12.1 ENSEMBL cdna:known chromosome:CEL140:II:3908492:3910830:-1 gene:F53C3.12 11/11 None
F53C3.12.2 ENSEMBL cdna:known chromosome:CEL140:II:3908537:3910832:-1 gene:F53C3.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3908535-3910952(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53C3.12
Entrez Gene 173733 Entrez gene
SwissProt Q9TXT9 EMBL-EBI
WormBase CE32672 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:253398_AT dioxygenase-related at
CANINE:1596653_AT similar to Beta,beta-carotene 9,10-dioxygenase (Beta-carotene dioxygenase 2) (B-diox-II) cfa
CANINE:1605569_AT similar to Beta,beta-carotene 9,10-dioxygenase (Beta-carotene dioxygenase 2) (B-diox-II) cfa
CANINE_2:CFA.2388.1.A1_AT similar to Beta,beta-carotene 9,10-dioxygenase (Beta-carotene dioxygenase 2) (B-diox-II) cfa
CANINE_2:CFA.2388.2.A1_AT similar to Beta,beta-carotene 9,10-dioxygenase (Beta-carotene dioxygenase 2) (B-diox-II) cfa
CANINE_2:CFA.2388.2.A1_S_AT similar to Beta,beta-carotene 9,10-dioxygenase (Beta-carotene dioxygenase 2) (B-diox-II) cfa
CANINE_2:CFAAFFX.21558.1.S1_S_AT similar to Beta,beta-carotene 9,10-dioxygenase (Beta-carotene dioxygenase 2) (B-diox-II) cfa
CANINE_2:CFAAFFX.21577.1.S1_AT similar to Beta,beta-carotene 9,10-dioxygenase (Beta-carotene dioxygenase 2) (B-diox-II) cfa
CANINE_2:CFAAFFX.21585.1.S1_AT similar to Beta,beta-carotene 9,10-dioxygenase (Beta-carotene dioxygenase 2) (B-diox-II) cfa
CHICKEN:GGAAFFX.4928.3.S1_S_AT similar to carotene-9,10-monooxygenase gga
U133_X3P:HS.181795.0.S1_3P_AT beta-carotene dioxygenase 2 hs
HG-U133_PLUS_2:232449_AT beta-carotene dioxygenase 2 hs
HG-U133B:232449_AT beta-carotene dioxygenase 2 hs
HG-U95E:85055_AT beta-carotene dioxygenase 2 hs
HG-U95D:89732_AT Beta-carotene dioxygenase 2 hs
MOE430A:1421221_AT beta-carotene 9', 10'-dioxygenase 2 mm
MOUSE430A_2:1421221_AT beta-carotene 9', 10'-dioxygenase 2 mm
MOUSE430_2:1421221_AT beta-carotene 9', 10'-dioxygenase 2 mm
MG-U74BV2:110214_AT beta-carotene 9', 10'-dioxygenase 2 mm
Protein Similarities
Method ID Description E-Value
blast AAC67462 Hypothetical protein F53C3.12 [Caenorhabditis elegans] ref|NP_494694.2| F53C3.12 [Caenorhabditis elegans] 0.0
blast F88115 protein F53C3.12 [imported] - Caenorhabditis elegans 0.0
blast CAE71509 Hypothetical protein CBG18441 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004294 EMBL-EBI Retinal pigment epithelial membrane protein 3.1E-59
Pfam IPR004294 EMBL-EBI Retinal pigment epithelial membrane protein 1.8E-11
Sequence
Target Sequence 
>C. ELEGANS:186270_S_AT
gccgggaattttgacgctttgttgattgagaacatgaagacgggaaatttccaaaacgat
gctctcttcctgccgtacctcaccagggtcattattcctctgtcaattccagacggtgcc
cagccgggtgatgatctgctgaagccgttgggctgggctaagggatgctctgcaatcttc
caggatgatgggaagattcgactgaaggagaagcgagtttgtgatatttctatggaattc
cctcgttatcactgggaaaagatcaacatgaagccgtacaactacgtgtatggctcaagt
gtacttggagcacaaaagtctgaaactcttcccggaatcgtgaaagccgatttagagaac
ggagatcacaaagtgtggcgtcgcgaaaatgataaacaaatctgcggagagcccattttt
gtgcccaatcccgaaggggttcgggaagatgacggaattctcatcgtcccagtgatgacg
ataagcgatggccaacgtccgttcgtcctaattcttgaagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCGGGAATTTTGACGCTTTGTTGA 531 279 1075 Antisense
TCACCAGGGTCATTATTCCTCTGTC 693 623 1154 Antisense
TCCTCTGTCAATTCCAGACGGTGCC 610 591 1170 Antisense
TGCCCAGCCGGGTGATGATCTGCTG 381 583 1191 Antisense
ATGATCTGCTGAAGCCGTTGGGCTG 521 45 1205 Antisense
TAAGGGATGCTCTGCAATCTTCCAG 263 637 1233 Antisense
TGGAATTCCCTCGTTATCACTGGGA 697 545 1307 Antisense
TGTGCCCAATCCCGAAGGGGTTCGG 196 533 1494 Antisense
ATGACGGAATTCTCATCGTCCCAGT 378 45 1523 Antisense
ACGATAAGCGATGGCCAACGTCCGT 699 91 1552 Antisense
GTCCGTTCGTCCTAATTCTTGAAGC 288 475 1571 Antisense
Sequence Source Affymetrix Proprietary Database