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[186269_s_at]

Description Y71F9B.6 /REP_DB=WormBase Gene ID /WP=CE25570 /TR=Q9N4H0 /GB=AAF36019.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 75      4grp_highest_8_8 > 17      4grp_high_8_8 > 60      6grp_highest_nonorm > 82      6grp_total > 76      4grp_total > 60      6grp_highest_sub > In      6grp_cgc > 57      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 370.6
(-0.5)
P 		476.7
(3)
P 		334.8
(-1.7)
P 		296.9
(-3)
P 		180
B 368
(0.1)
P 		356
(-0.4)
P 		438.2
(3)
P 		292.3
(-3)
P 		146
C 379.1
(1)
P 		309.7
(-3)
P 		412.7
(3)
P 		380.7
(1.1)
P 		103
avr 372.6
(1.1) 		380.8
(1.8) 		395.2
(3) 		323.3
(-3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 307
1 2 3 4 5 6 range
A 301.8
(-3)
P 		346.5
(-1.8)
P 		398.6
(-0.4)
P 		439.2
(0.8)
P 		373.8
(-1)
P 		521.6
(3)
P 		220
B 277.4
(-3)
P 		354.7
(-1)
P 		381.5
(-0.3)
P 		342
(-1.3)
P 		346.1
(-1.2)
P 		511.2
(3)
P 		234
C 413.8
(-0.6)
P 		296.8
(-3)
P 		409
(-0.7)
P 		387.1
(-1.1)
P 		385.8
(-1.1)
P 		584.1
(3)
P 		287
avr 331
(-3) 		332.7
(-3) 		396.4
(-1.1) 		389.4
(-1.3) 		368.6
(-1.9) 		539
(3) 		208
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 100
1 2 3 4 range
A 135
(3)
P 		103.5
(0.9)
P 		87.5
(-0.1)
P 		43.5
(-3)
P 		92
B 117.4
(3)
P 		87.9
(-0.2)
P 		101.5
(1.3)
P 		62.3
(-3)
P 		55
C 125.5
(1.6)
P 		89.1
(-1.1)
P 		143.8
(3)
P 		64.2
(-3)
P 		80
avr 126
(3) 		93.5
(0.2) 		110.9
(1.7) 		56.7
(-3) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 140
1 2 3 4 5 6 range
A 55.6
(-3)
P 		123.3
(2.7)
P 		86.8
(-0.4)
P 		111.4
(1.7)
P 		112.8
(1.8)
P 		127
(3)
P 		71
B 96.5
(-2.6)
P 		118.1
(-1.4)
P 		92.6
(-2.9)
P 		90.6
(-3)
P 		140.6
(0)
P 		190.6
(3)
P 		100
C 153.4
(0.9)
P 		90.6
(-2.4)
P 		104.1
(-1.7)
P 		77.9
(-3)
P 		124.8
(-0.6)
P 		195.5
(3)
P 		118
avr 101.8
(-2.3) 		110.7
(-1.7) 		94.5
(-2.9) 		93.3
(-3) 		126.1
(-0.5) 		171
(3) 		78
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186269_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.41
Sequence Type Exemplar sequence
Representative Public ID Y71F9B.6 NCBI
Target Description Y71F9B.6 /REP_DB=WormBase Gene ID /WP=CE25570 /TR=Q9N4H0 /GB=AAF36019.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058629(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058629 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-30) (dnj-30) mRNA, complete cds. 11/11 None
SNAP00000013303 ENSEMBL cdna:SNAP chromosome:CEL140:I:2716627:2726652:-1 11/11 None
Y71F9B.16 ENSEMBL cdna:known chromosome:CEL140:I:2718091:2721470:-1 gene:Y71F9B.16 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000013345 4/11 Cross Hyb Matching Probes None
GENEFINDER00000013359 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2718128-2726741(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dnj-30
Ensembl Y71F9B.16
Entrez Gene 171833 Entrez gene
SwissProt Q95Y44 EMBL-EBI
WormBase CE29000 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1602273_AT similar to DnaJ (Hsp40) homolog, subfamily C, member 8 cfa
CANINE:1586866_S_AT similar to DnaJ (Hsp40) homolog, subfamily C, member 8 cfa
CANINE_2:CFA.671.1.A1_S_AT similar to DnaJ (Hsp40) homolog, subfamily C, member 8 cfa
DROSOPHILA_2:1628615_AT dm
DROSGENOME1:153613_AT dm
CHICKEN:GGA.6131.1.S1_AT DnaJ (Hsp40) homolog, subfamily C, member 8 gga
CHICKEN:GGAAFFX.22149.1.S1_AT DnaJ (Hsp40) homolog, subfamily C, member 8 gga
HG-U133A:212491_S_AT DnaJ (Hsp40) homolog, subfamily C, member 8 hs
HG-U133A_2:212491_S_AT DnaJ (Hsp40) homolog, subfamily C, member 8 hs
HG-FOCUS:212491_S_AT DnaJ (Hsp40) homolog, subfamily C, member 8 hs
HG-U133_PLUS_2:212491_S_AT DnaJ (Hsp40) homolog, subfamily C, member 8 hs
HU35KSUBA:N98656_S_AT DnaJ (Hsp40) homolog, subfamily C, member 8 hs
HG-U133A:205545_X_AT DnaJ (Hsp40) homolog, subfamily C, member 8 hs
HG-U133_PLUS_2:205545_X_AT DnaJ (Hsp40) homolog, subfamily C, member 8 hs
HG-U133A_2:205545_X_AT DnaJ (Hsp40) homolog, subfamily C, member 8 hs
U133_X3P:G7657610_3P_A_AT DnaJ (Hsp40) homolog, subfamily C, member 8 hs
HG-U133A_2:212490_AT DnaJ (Hsp40) homolog, subfamily C, member 8 hs
HG-U133_PLUS_2:212490_AT DnaJ (Hsp40) homolog, subfamily C, member 8 hs
HG-U133A:212490_AT DnaJ (Hsp40) homolog, subfamily C, member 8 hs
HU35KSUBC:RC_W37375_AT DnaJ (Hsp40) homolog, subfamily C, member 8 hs
U133_X3P:HS.74711.3.S1_3P_AT DnaJ (Hsp40) homolog, subfamily C, member 8 hs
HG-U95AV2:36166_AT DnaJ (Hsp40) homolog, subfamily C, member 8 hs
MG-U74AV2:95700_R_AT DnaJ (Hsp40) homolog, subfamily C, member 8 mm
MG-U74AV2:95699_F_AT DnaJ (Hsp40) homolog, subfamily C, member 8 mm
MOE430A:1452683_AT DnaJ (Hsp40) homolog, subfamily C, member 8 mm
MOUSE430A_2:1452683_AT DnaJ (Hsp40) homolog, subfamily C, member 8 mm
MOUSE430_2:1452683_AT DnaJ (Hsp40) homolog, subfamily C, member 8 mm
MOE430B:1431123_S_AT DnaJ (Hsp40) homolog, subfamily C, member 8 mm
MOUSE430_2:1431123_S_AT DnaJ (Hsp40) homolog, subfamily C, member 8 mm
MG-U74AV2:93199_AT L41 ribosomal like-protein mm
MOE430A:1452488_AT L41 ribosomal like-protein mm
MOUSE430_2:1452488_AT L41 ribosomal like-protein mm
MOUSE430A_2:1452488_AT L41 ribosomal like-protein mm
MU19KSUBB:TC31672_AT L41 ribosomal like-protein mm
MU19KSUBB:TC31672_G_AT L41 ribosomal like-protein mm
RG-U34B:RC_AA925152_AT DnaJ (Hsp40) homolog, subfamily C, member 8 (predicted) rn
RAE230A:1367492_AT DnaJ (Hsp40) homolog, subfamily C, member 8 (predicted) rn
RAE230B:1367492_AT DnaJ (Hsp40) homolog, subfamily C, member 8 (predicted) rn
RAT230_2:1367492_AT DnaJ (Hsp40) homolog, subfamily C, member 8 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAE60495 Hypothetical protein CBG04113 [Caenorhabditis briggsae] 0.0
blast AAK93867 Hypothetical protein Y71F9B.6 [Caenorhabditis elegans] ref|NP_491031.1| Y71F9B.6 [Caenorhabditis elegans] 0.0
blast AAK93866 Dnaj domain (prokaryotic heat shock protein) protein 30 [Caenorhabditis elegans] ref|NP_491030.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-30) [Caenorhabditis elegans] 1.0E-116
blast CAE60495 Hypothetical protein CBG04113 [Caenorhabditis briggsae] 1.0E-110
blast AAK93866 Dnaj domain (prokaryotic heat shock protein) protein 30 [Caenorhabditis elegans] ref|NP_491030.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-30) [Caenorhabditis elegans] 1.0E-107
blast EAA04821 ENSANGP00000019596 [Anopheles gambiae str. PEST] ref|XP_309089.2| ENSANGP00000019596 [Anopheles gambiae str. PEST] 5.0E-54
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 0.660000026226044
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 0.0309999994933605
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 2.3E-12
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 2.3E-12
Sequence
Target Sequence 
>C. ELEGANS:186269_S_AT
tttgaagcccggttccacatatttgaaccttaacccatatgaagtgctccaaatcgattt
ggacacggatatcgaggcggctaagaagaaatacaagaagctctcactacttgtgcatcc
ggataaaaatccagatgatcgagaacgtgctgacaaagcttttgatattgtgaagaaggc
gattaagcaaatggaggatccgattgagttgaatcgatgcaaagattgttatacggaggg
aaaagctcggcttgctattgtgatgtcggagaaaaagcgaaaattgcgaaaagatggaaa
agaagatgtgattgaagaagatgatccagctggatacaagaaagctctgtggattactgt
aacaaaagtgtttgctgatcgagagaagaagagaaagatgcttgaagagagggcgaatga
ggaaaaacgccgctcaactgaagcgttagtccaagcagcagagaagcgtaaactggccga
agaattcgcgaaaaactacgaagaatctcgtgacgaacgttccggaagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTGAAGCCCGGTTCCACATATTTG 83 669 1716 Antisense
TCCACATATTTGAACCTTAACCCAT 643 585 1729 Antisense
AACCCATATGAAGTGCTCCAAATCG 655 145 1747 Antisense
TGGACACGGATATCGAGGCGGCTAA 14 545 1775 Antisense
CTCTCACTACTTGTGCATCCGGATA 17 231 1816 Antisense
ATGATCGAGAACGTGCTGACAAAGC 26 47 1850 Antisense
GCTCGGCTTGCTATTGTGATGTCGG 586 299 1960 Antisense
AGAAAGCTCTGTGGATTACTGTAAC 556 71 2054 Antisense
ACGCCGCTCAACTGAAGCGTTAGTC 128 91 2142 Antisense
GAAGCGTTAGTCCAAGCAGCAGAGA 350 341 2155 Antisense
ATCTCGTGACGAACGTTCCGGAAGC 33 25 2220 Antisense
Sequence Source Affymetrix Proprietary Database