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[186267_at]

Description H14E04.1 /REP_DB=WormBase Gene ID /WP=CE20965 /TR=Q9TYP1 /GB=AAD12813.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 09      4grp_total > 50      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 415
1 2 3 4 range
A 226.8
(-1.7)
A 		521.9
(3)
P 		438.5
(1.7)
A 		144.7
(-3)
A 		377
B 125
(-2.7)
A 		484.1
(3)
P 		364.2
(1.1)
A 		107.2
(-3)
A 		377
C 246.8
(-1)
A 		518.7
(3)
P 		468.8
(2.3)
P 		109.4
(-3)
A 		409
avr 199.5
(-1.8) 		508.2
(3) 		423.8
(1.7) 		120.4
(-3) 		388

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 470
1 2 3 4 5 6 range
A 466.5
(3)
P 		312.6
(-0.2)
P 		417.6
(2)
A 		461.2
(2.9)
P 		326.3
(0.1)
P 		180.4
(-3)
M 		286
B 608.6
(2.8)
A 		360.4
(-0.4)
P 		367.8
(-0.3)
A 		623.9
(3)
P 		353.1
(-0.5)
P 		154.3
(-3)
A 		470
C 546.4
(3)
A 		326.1
(-0.4)
A 		336.4
(-0.3)
P 		419.8
(1)
P 		338.7
(-0.2)
A 		161
(-3)
A 		385
avr 540.5
(3) 		333
(-0.3) 		373.9
(0.3) 		501.6
(2.4) 		339.4
(-0.2) 		165.2
(-3) 		375
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 145
1 2 3 4 range
A 82.6
(0.9)
A 		113.3
(2.9)
P 		114.6
(3)
A 		21.2
(-3)
A 		93
B 39.9
(-1.9)
A 		119.6
(3)
P 		84.4
(0.8)
A 		22.9
(-3)
A 		97
C 81.7
(-0.4)
A 		149.1
(2.4)
P 		163.4
(3)
P 		18.4
(-3)
A 		145
avr 68.1
(-0.3) 		127.3
(3) 		120.8
(2.6) 		20.8
(-3) 		107

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 168
1 2 3 4 5 6 range
A 86
(0.5)
P 		111.2
(2.5)
P 		91
(0.9)
A 		117
(3)
P 		98.5
(1.5)
P 		43.9
(-3)
M 		73
B 211.8
(3)
A 		119.9
(-0.6)
P 		89.3
(-1.8)
A 		165.2
(1.2)
P 		143.5
(0.3)
P 		57.5
(-3)
A 		154
C 202.5
(3)
A 		99.5
(-1.2)
A 		85.6
(-1.7)
P 		84.4
(-1.8)
P 		109.6
(-0.8)
A 		53.9
(-3)
A 		149
avr 166.8
(3) 		110.2
(0) 		88.6
(-1.1) 		122.2
(0.7) 		117.2
(0.4) 		51.8
(-3) 		115
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186267_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6180
Sequence Type Exemplar sequence
Representative Public ID H14E04.1 NCBI
Target Description H14E04.1 /REP_DB=WormBase Gene ID /WP=CE20965 /TR=Q9TYP1 /GB=AAD12813.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065148(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065148 NCBI Caenorhabditis elegans H14E04.1 (H14E04.1) mRNA, complete cds. 10/11 None
H14E04.1 ENSEMBL cdna:known chromosome:CEL140:III:2402067:2406253:1 gene:H14E04.1 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000019367 5/11 Cross Hyb Matching Probes None
GENEFINDER00000019379 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2402086-2406244(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H14E04.1
Entrez Gene 175358 Entrez gene
SwissProt Q9TYP1 EMBL-EBI
WormBase CE29973 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:261727_AT S-adenosyl-methionine-sterol-C-methyltransferase at
ATGENOME1:15198_AT S-adenosyl-methionine-sterol-C-methyltransferase at
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
8168 methyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAD12813 Hypothetical protein H14E04.1 [Caenorhabditis elegans] ref|NP_497549.2| H14E04.1 [Caenorhabditis elegans] 0.0
blast CAE69462 Hypothetical protein CBG15658 [Caenorhabditis briggsae] 1.0E-171
blast AAX07631 sterol 24-C-methyltransferase-like protein [Magnaporthe grisea] sp|Q5EN22|ERG6_MAGGR Sterol 24-C-methyltransferase (Delta(24)-sterol C-methyltransferase) 7.0E-30
Sequence
Target Sequence 
>C. ELEGANS:186267_AT
agattcgacatttgacgttgcttatgctatatattccctgaaatatatcccgaatttgga
caaagttatgaaggaaattcagagagtgctcaagccgggaggaaaatttattgtatacga
tttgataaagacaaatgattatgacaaggataataaggaacattataagattttaagaaa
aaaaattttcgccaaaaattttttttttgaaaattccacaaaaaaaattaaacttttttt
agccaaaaaatcaaatgctttccagaccctccatcatctggaatacgcgtgcggaatgcc
atcacttcatacccaatcagaagtcgaagcggccgccgaaaaatgggaaatgcctgtggt
ggagcgggaaaatttggaggaaacctacggaaatcgggcttttcactactgtttctcggc
gagcccaatgttcatgtggttggtgagcagtccagtgatagatcacactatccgaatggc
tgaaattttgcgcatattacccgccgggtttaaacaatttaaccgaacttttctgtgtgg
aacagtgaactcaattgtcggaggaggacggatgggaatactctccggagccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGATTCGACATTTGACGTTGCTTAT 703 65 489 Antisense
GTTGCTTATGCTATATATTCCCTGA 511 439 505 Antisense
AAAATCAAATGCTTTCCAGACCCTC 648 133 735 Antisense
GACCCTCCATCATCTGGAATACGCG 468 381 753 Antisense
ATACGCGTGCGGAATGCCATCACTT 520 23 771 Antisense
GGGAAATGCCTGTGGTGGAGCGGGA 331 493 833 Antisense
GAAATCGGGCTTTTCACTACTGTTT 360 361 878 Antisense
TACTGTTTCTCGGCGAGCCCAATGT 12 643 895 Antisense
TAGATCACACTATCCGAATGGCTGA 225 653 947 Antisense
AAATTTTGCGCATATTACCCGCCGG 498 115 971 Antisense
CGGATGGGAATACTCTCCGGAGCCG 611 241 1057 Antisense
Sequence Source Affymetrix Proprietary Database