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[186256_at]

Description T25F10.5 /REP_DB=WormBase Gene ID /WP=CE07536 /TR=Q23049 /GB=AAB04988.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 08      4grp_total > 31      6grp_cgc > 10      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 179.2
(3)
P 		52.8
(-1.6)
A 		15.5
(-3)
A 		80.7
(-0.6)
A 		164
B 169.7
(3)
P 		15.1
(-3)
A 		14.8
(-3)
A 		69.1
(-0.9)
P 		155
C 192.2
(3)
P 		26.2
(-2.4)
A 		7.9
(-3)
A 		165
(2.1)
P 		184
avr 180.4
(3) 		31.4
(-2.3) 		12.7
(-3) 		104.9
(0.3) 		168

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 123
1 2 3 4 5 6 range
A 127.1
(3)
P 		33.4
(-1.6)
A 		29.8
(-1.8)
A 		52
(-0.7)
A 		4.3
(-3)
A 		12.9
(-2.6)
A 		123
B 99.1
(3)
P 		27.1
(-2)
A 		49
(-0.5)
A 		46.2
(-0.7)
A 		12.9
(-3)
A 		13.7
(-2.9)
A 		86
C 28
(-0.6)
A 		55.2
(3)
A 		35.4
(0.4)
A 		39.6
(0.9)
A 		13.6
(-2.5)
A 		9.9
(-3)
A 		45
avr 84.7
(3) 		38.6
(-0.7) 		38.1
(-0.8) 		45.9
(-0.1) 		10.3
(-3) 		12.2
(-2.8) 		74
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 65.2
(3)
P 		11.5
(-2.3)
A 		4
(-3)
A 		11.8
(-2.2)
A 		61
B 54.1
(3)
P 		3.7
(-3)
A 		3.4
(-3)
A 		14.7
(-1.7)
P 		51
C 63.6
(3)
P 		7.5
(-2.5)
A 		2.7
(-3)
A 		27.8
(-0.5)
P 		61
avr 61
(3) 		7.6
(-2.6) 		3.4
(-3) 		18.1
(-1.5) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 23.4
(3)
P 		11.9
(-0.1)
A 		6.5
(-1.6)
A 		13.2
(0.2)
A 		1.3
(-3)
A 		3.1
(-2.5)
A 		22
B 34.5
(3)
P 		9
(-2.2)
A 		11.9
(-1.6)
A 		12.2
(-1.6)
A 		5.2
(-3)
A 		5.1
(-3)
A 		29
C 10.4
(0.2)
A 		16.8
(3)
A 		9
(-0.5)
A 		8
(-0.9)
A 		4.4
(-2.5)
A 		3.3
(-3)
A 		14
avr 22.8
(3) 		12.6
(-0.2) 		9.1
(-1.3) 		11.1
(-0.7) 		3.6
(-3) 		3.8
(-2.9) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186256_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11824
Sequence Type Exemplar sequence
Representative Public ID T25F10.5 NCBI
Target Description T25F10.5 /REP_DB=WormBase Gene ID /WP=CE07536 /TR=Q23049 /GB=AAB04988.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072310(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072310 NCBI Caenorhabditis elegans BBS (Bardet-Biedl Syndrome) protein family member (bbs-8) (bbs-8) mRNA, complete cds. 11/11 None
SNAP00000017680 ENSEMBL cdna:SNAP chromosome:CEL140:V:6765204:6767611:-1 11/11 None
GENEFINDER00000017689 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6765204:6767689:-1 11/11 None
T25F10.5 ENSEMBL cdna:known chromosome:CEL140:V:6765070:6767666:-1 gene:T25F10.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6765204-6767246(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol bbs-8
Ensembl T25F10.5
Entrez Gene 188904 Entrez gene
SwissProt Q23049 EMBL-EBI
WormBase CE31071 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.448.1.S1_AT similar to tetratricopeptide repeat domain 8 isoform A cfa
CANINE_2:CFAAFFX.26740.1.S1_S_AT similar to tetratricopeptide repeat domain 8 isoform A cfa
DROSOPHILA_2:1627476_AT dm
DROSGENOME1:145530_AT dm
CHICKEN:GGAAFFX.6738.1.S1_AT similar to tetratricopeptide repeat domain 8 isoform 1; Bardet-Biedl syndrome type 8 gga
CHICKEN:GGAAFFX.6738.2.S1_S_AT similar to tetratricopeptide repeat domain 8 isoform 1; Bardet-Biedl syndrome type 8 gga
HU35KSUBC:RC_AA405620_AT tetratricopeptide repeat domain 8 hs
U133_X3P:HS.55158.0.A1_3P_AT tetratricopeptide repeat domain 8 hs
HG-U133_PLUS_2:226120_AT tetratricopeptide repeat domain 8 hs
HG-U133B:226120_AT tetratricopeptide repeat domain 8 hs
HG-U95C:62208_R_AT tetratricopeptide repeat domain 8 hs
HG-U133_PLUS_2:237474_AT Tetratricopeptide repeat domain 8 hs
HG-U133B:237474_AT Tetratricopeptide repeat domain 8 hs
HG-U133_PLUS_2:240485_AT Tetratricopeptide repeat domain 8 hs
HG-U133B:240485_AT Tetratricopeptide repeat domain 8 hs
HG-U95C:59004_R_AT Tetratricopeptide repeat domain 8 hs
HG-U95D:70730_R_AT Tetratricopeptide repeat domain 8 hs
HU35KSUBB:RC_AA018587_AT Tetratricopeptide repeat domain 8 hs
U133_X3P:HS.211122.0.A1_3P_AT Tetratricopeptide repeat domain 8 hs
U133_X3P:HS.250610.0.A1_3P_AT Tetratricopeptide repeat domain 8 hs
MG-U74BV2:163404_AT tetratricopeptide repeat domain 8 mm
MOE430A:1424410_AT tetratricopeptide repeat domain 8 mm
MOUSE430A_2:1424410_AT tetratricopeptide repeat domain 8 mm
MOUSE430_2:1424410_AT tetratricopeptide repeat domain 8 mm
MU19KSUBA:TC17314_AT tetratricopeptide repeat domain 8 mm
RAE230A:1378416_AT Tetratricopeptide repeat domain 8 (predicted) rn
RAT230_2:1378416_AT Tetratricopeptide repeat domain 8 (predicted) rn
RG-U34A:RC_AA892565_AT Tetratricopeptide repeat domain 8 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4289 subtilase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB04988 Bbs (bardet-biedl syndrome) protein protein 8 [Caenorhabditis elegans] ref|NP_504711.2| BBS (Bardet-Biedl Syndrome) protein family member (bbs-8) [Caenorhabditis elegans] 0.0
blast CAE71946 Hypothetical protein CBG19013 [Caenorhabditis briggsae] 0.0
blast XP_785839 PREDICTED: similar to tetratricopeptide repeat domain 8 isoform B [Strongylocentrotus purpuratus] 1.0E-117
Protein Domains
Database ID Description E-Value
Pfam IPR001440 EMBL-EBI TPR repeat 6.2E-4
Pfam IPR001440 EMBL-EBI TPR repeat 0.4
Pfam IPR001440 EMBL-EBI TPR repeat 8.8E-7
Pfam IPR001440 EMBL-EBI TPR repeat 0.0045
Pfam IPR001440 EMBL-EBI TPR repeat 4.5E-4
Pfam IPR001440 EMBL-EBI TPR repeat 6.2E-4
Pfam IPR001440 EMBL-EBI TPR repeat 0.4
Pfam IPR001440 EMBL-EBI TPR repeat 8.8E-7
Pfam IPR001440 EMBL-EBI TPR repeat 0.0045
Pfam IPR001440 EMBL-EBI TPR repeat 4.5E-4
Sequence
Target Sequence 
>C. ELEGANS:186256_AT
gaaaacctgaactcgctatgcgttactacagaagaattctgcagatgggtgtttcgtcgc
ctgaacttttcttgaatattgggctgtgctgcatggcagctcaacagtttgatttcgcgc
tctcgtctatactcagggcccaatccaccatgacggatgacgttgcggcagatgtttggt
ataacataggccaaattttagtggatattggtgatctagtttctgccgcaagatcatttc
ggattgctctttctcatgatccagatcatagtgaatctcttgtcaatcttggaattctga
aacatagagagggtaaaatcgatgaagcccgttccttgtatagttctgcgacttcaaaaa
atccgtacatgtttgagggaaactacaacctgggtctcgtctcattcacacaaggaaaat
accacgaatgtcgagaattaatcgaaaaagcgctcgcagcgttcccagagcatgagcatt
g

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAACCTGAACTCGCTATGCGTTA 51 353 863 Antisense
TTCTGCAGATGGGTGTTTCGTCGCC 19 693 899 Antisense
TTCGTCGCCTGAACTTTTCTTGAAT 443 693 915 Antisense
GAATATTGGGCTGTGCTGCATGGCA 60 323 936 Antisense
CTCAACAGTTTGATTTCGCGCTCTC 94 229 962 Antisense
TATTGGTGATCTAGTTTCTGCCGCA 538 665 1068 Antisense
ATTTCGGATTGCTCTTTCTCATGAT 158 17 1098 Antisense
AAAATCGATGAAGCCCGTTCCTTGT 411 133 1177 Antisense
CCCGTTCCTTGTATAGTTCTGCGAC 351 259 1190 Antisense
TCGAAAAAGCGCTCGCAGCGTTCCC 71 599 1304 Antisense
CAGCGTTCCCAGAGCATGAGCATTG 272 203 1319 Antisense
Sequence Source Affymetrix Proprietary Database