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[186255_at]

Description F39H12.1 /REP_DB=WormBase Gene ID /WP=CE27160 /TR=Q20198 /GB=AAA83604.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 62      4grp_high_8_8 > 37      6grp_highest_nonorm > 90      6grp_total > 72      4grp_total > 50      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 56.8
(-0.7)
P 		108.3
(3)
P 		84.6
(1.3)
M 		23.9
(-3)
A 		84
B 54.2
(-3)
A 		94.3
(3)
P 		87.3
(2)
P 		59.4
(-2.2)
P 		40
C 73.7
(-3)
P 		93.4
(3)
M 		79.5
(-1.2)
P 		90.3
(2.1)
P 		20
avr 61.6
(-2.5) 		98.7
(3) 		83.8
(0.8) 		57.9
(-3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 115
1 2 3 4 5 6 range
A 142.3
(3)
P 		129.7
(0.8)
P 		141.3
(2.8)
P 		139.3
(2.5)
P 		139.3
(2.5)
P 		108.4
(-3)
P 		34
B 119.1
(-2.9)
P 		126.8
(-1.9)
P 		165
(3)
P 		118
(-3)
P 		150.2
(1.1)
P 		152
(1.3)
P 		47
C 87.8
(-3)
P 		155.4
(0.5)
P 		138.5
(-0.3)
P 		202.5
(3)
P 		107.2
(-2)
P 		114.9
(-1.6)
P 		115
avr 116.4
(-3) 		137.3
(0.4) 		148.3
(2.2) 		153.3
(3) 		132.2
(-0.4) 		125.1
(-1.6) 		37
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 20.7
(2.2)
P 		23.5
(3)
P 		22.1
(2.6)
M 		3.5
(-3)
A 		20
B 17.3
(-0.4)
A 		23.3
(3)
P 		20.2
(1.2)
P 		12.7
(-3)
P 		11
C 24.4
(1.4)
P 		26.9
(2.6)
M 		27.7
(3)
P 		15.2
(-3)
P 		13
avr 20.8
(1.4) 		24.6
(3) 		23.3
(2.4) 		10.5
(-3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 26.2
(-3)
P 		46.1
(3)
P 		30.8
(-1.6)
P 		35.3
(-0.3)
P 		42
(1.8)
P 		26.4
(-2.9)
P 		20
B 41.5
(-0.9)
P 		42.2
(-0.8)
P 		40.1
(-1.2)
P 		31.2
(-3)
P 		61
(3)
P 		56.7
(2.1)
P 		30
C 32.6
(-3)
P 		47.4
(3)
P 		35.2
(-1.9)
P 		40.7
(0.3)
P 		34.7
(-2.1)
P 		38.5
(-0.6)
P 		15
avr 33.4
(-3) 		45.2
(2.7) 		35.4
(-2) 		35.7
(-1.9) 		45.9
(3) 		40.5
(0.4) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186255_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18525
Sequence Type Exemplar sequence
Representative Public ID F39H12.1 NCBI
Target Description F39H12.1 /REP_DB=WormBase Gene ID /WP=CE27160 /TR=Q20198 /GB=AAA83604.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075763(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075763 NCBI Caenorhabditis elegans F39H12.1 (F39H12.1) mRNA, complete cds. 11/11 None
SNAP00000043077 ENSEMBL cdna:SNAP chromosome:CEL140:X:834788:837670:1 11/11 None
GENEFINDER00000043081 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:834788:837670:1 11/11 None
F39H12.1 ENSEMBL cdna:known chromosome:CEL140:X:834777:837757:1 gene:F39H12.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:834787-837670(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F39H12.1
Entrez Gene 180432 Entrez gene
SwissProt Q20198 EMBL-EBI
WormBase CE35527 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA83604 Hypothetical protein F39H12.1 [Caenorhabditis elegans] ref|NP_508164.2| F39H12.1 [Caenorhabditis elegans] 0.0
blast CAE63613 Hypothetical protein CBG08106 [Caenorhabditis briggsae] 1.0E-112
blast XP_657028 myosin heavy chain [Entamoeba histolytica HM-1:IMSS] gb|EAL51645.1| myosin heavy chain [Entamoeba histolytica HM-1:IMSS] 5.0E-9
Sequence
Target Sequence 
>C. ELEGANS:186255_AT
agcacagattcgtggcacagaactcaagatgtacttcaatattttagaaaccagatctca
caaatgaaaggtgcgctacaaaaaacagaagaaacatttatcagtgatttcgaaactttt
ggagcagaaattaaaagaagcttggagaatcaagcgaagaggaaatcctcatcaccgaag
attatacaattaccctgtaactgtgaaaacaagtctcgggagcaaacgaatcaagtcgtc
aaacatgatgaatctacacaaactaaatcagtatacattgatgaaagccatcggtgggag
agagaagaaaaggaaatcgcatcttctccaaatggcaagaacgggaatgtgaccgacctt
ctgcagataattgaacaattagagatggaaaagagacttcttttggcgcagagacgaagt
gataaagcggaaattgagaggctgcaagaacaactcaatgagaccttaatcatgcatggt
gaaaagatcgagcaacttgatgaaatcctcgtggacaacgaagaattgaaagatattttg
aagcaacaagcgttggctctcacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCACAGATTCGTGGCACAGAACTC 304 77 685 Antisense
GGCACAGAACTCAAGATGTACTTCA 551 535 698 Antisense
GTGATTTCGAAACTTTTGGAGCAGA 569 483 788 Antisense
GAGGAAATCCTCATCACCGAAGATT 3 405 843 Antisense
AAATCGCATCTTCTCCAAATGGCAA 626 117 998 Antisense
AGAACGGGAATGTGACCGACCTTCT 579 67 1022 Antisense
TGTGACCGACCTTCTGCAGATAATT 567 555 1032 Antisense
AGAGACTTCTTTTGGCGCAGAGACG 411 69 1076 Antisense
GGCTGCAAGAACAACTCAATGAGAC 676 539 1124 Antisense
GCAACTTGATGAAATCCTCGTGGAC 684 319 1176 Antisense
GAAGCAACAAGCGTTGGCTCTCACG 285 341 1224 Antisense
Sequence Source Affymetrix Proprietary Database