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[186248_at]

Description F54D10.3 /REP_DB=WormBase Gene ID /WP=CE19453 /TR=Q9TZ87 /GB=AAC68781.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 18      6grp_high1_10_10 > 81      4grp_highest_8_8 > 38      4grp_high_8_8 > 04      6grp_highest_nonorm > 72      6grp_total > 81      4grp_total > 05      6grp_highest_sub > 04_05      6grp_high1_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 381
1 2 3 4 range
A 389.3
(1.1)
P 		209.4
(-3)
P 		280.1
(-1.4)
P 		474.2
(3)
P 		265
B 314.7
(-1.2)
P 		219.2
(-3)
P 		244.7
(-2.5)
P 		544.7
(3)
P 		326
C 291.8
(-1.3)
P 		211.5
(-2.6)
P 		185.6
(-3)
P 		567
(3)
P 		381
avr 331.9
(-0.7) 		213.4
(-3) 		236.8
(-2.6) 		528.6
(3) 		315

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 332
1 2 3 4 5 6 range
A 353.1
(-1.7)
P 		329.4
(-2.2)
P 		354.8
(-1.6)
P 		550.9
(3)
P 		537.9
(2.7)
P 		297
(-3)
P 		254
B 315
(-1.2)
P 		405.1
(1.4)
P 		370.3
(0.4)
P 		461.4
(3)
P 		427.1
(2)
P 		251.7
(-3)
P 		210
C 317.1
(-1.6)
P 		449.6
(0.8)
P 		405.8
(0)
P 		571.5
(3)
P 		394.4
(-0.2)
P 		239.1
(-3)
P 		332
avr 328.4
(-1.5) 		394.7
(-0) 		377
(-0.4) 		527.9
(3) 		453.1
(1.3) 		262.6
(-3) 		265
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 141.7
(3)
P 		45.4
(-3)
P 		73.2
(-1.3)
P 		69.4
(-1.5)
P 		96
B 100.4
(1.5)
P 		54.1
(-3)
P 		56.7
(-2.7)
P 		116.1
(3)
P 		62
C 96.6
(3)
P 		60.8
(-3)
P 		64.7
(-2.3)
P 		95.6
(2.8)
P 		36
avr 112.9
(3) 		53.4
(-3) 		64.9
(-1.8) 		93.7
(1.1) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 65.1
(-3)
P 		117.1
(0.2)
P 		77.3
(-2.2)
P 		139.7
(1.6)
P 		162.3
(3)
P 		72.3
(-2.6)
P 		97
B 109.6
(-1.6)
P 		134.8
(0.2)
P 		89.9
(-3)
P 		122.2
(-0.7)
P 		173.5
(3)
P 		93.9
(-2.7)
P 		84
C 117.6
(0.9)
P 		137.2
(3)
P 		103.3
(-0.6)
P 		114.9
(0.7)
P 		127.6
(2)
P 		80
(-3)
P 		57
avr 97.4
(-1.7) 		129.7
(0.9) 		90.2
(-2.3) 		125.6
(0.6) 		154.5
(3) 		82.1
(-3) 		72
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186248_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4294
Sequence Type Exemplar sequence
Representative Public ID F54D10.3 NCBI
Target Description F54D10.3 /REP_DB=WormBase Gene ID /WP=CE19453 /TR=Q9TZ87 /GB=AAC68781.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062257(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062257 NCBI Caenorhabditis elegans F54D10.3 (F54D10.3) mRNA, complete cds. 11/11 None
SNAP00000030971 ENSEMBL cdna:SNAP chromosome:CEL140:II:3809257:3813339:1 11/11 None
GENEFINDER00000030987 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3812299:3813339:1 11/11 None
F54D10.3 ENSEMBL cdna:known chromosome:CEL140:II:3812299:3813339:1 gene:F54D10.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3812297-3813338(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54D10.3
Entrez Gene 173726 Entrez gene
SwissProt Q9TZ87 EMBL-EBI
WormBase CE19453 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68781 Hypothetical protein F54D10.3 [Caenorhabditis elegans] ref|NP_494658.1| F54D10.3 [Caenorhabditis elegans] 1.0E-166
blast AAB70932 Hypothetical protein C41H7.4 [Caenorhabditis elegans] ref|NP_494422.1| C41H7.4 [Caenorhabditis elegans] 1.0E-21
blast AAB70932 Hypothetical protein C41H7.4 [Caenorhabditis elegans] ref|NP_494422.1| C41H7.4 [Caenorhabditis elegans] 2.0E-21
blast CAE59326 Hypothetical protein CBG02668 [Caenorhabditis briggsae] 3.0E-20
Protein Domains
Database ID Description E-Value
Pfam IPR005020 EMBL-EBI Protein of unknown function DUF278 1.0E-126
Pfam IPR005020 EMBL-EBI Protein of unknown function DUF278 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000030971 1 83-105
Sequence
Target Sequence 
>C. ELEGANS:186248_AT
gctgttccaggatcttcggttttactatgacaagctttacaagtatcggcagttgcgagc
agaacgaacagggaatgaggcgaggaattcggagcccgtcagcggagaggatatgcaatc
gttcttcgaagagcccgatatgatgtcgttggttgaggagaatgatagcgccatgaagta
cctctcatctcttattccacaagtctcatcgacctcgactcccaccaaggttaagctgga
gaatccatttctcaatgctttcgaaaacccgatgaatctgaggcatgagctgagtcagat
gctccaggcaagctcgtcgagttcttcaataccttcacctatctctccaccagtgccgaa
tccaaaagcagaggatgaggagaagccgtctctaaaacgccctcgagaatccagcccagc
ggcagtagagacagaacgaataagccagcaaatcaaacgaattttcgagcaatatcccga
gaaaaccaatctgatccggtctgtgctcacctacaccgtgcttgcttttgatgagcccga
cgcggatttttcgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTGTTCCAGGATCTTCGGTTTTAC 547 293 330 Antisense
GAGGCGAGGAATTCGGAGCCCGTCA 440 405 406 Antisense
ATGAAGTACCTCTCATCTCTTATTC 513 43 502 Antisense
TTATTCCACAAGTCTCATCGACCTC 363 675 521 Antisense
CTCGACTCCCACCAAGGTTAAGCTG 348 231 543 Antisense
AGTCAGATGCTCCAGGCAAGCTCGT 405 59 622 Antisense
GGAGAAGCCGTCTCTAAAACGCCCT 613 511 708 Antisense
GAATCCAGCCCAGCGGCAGTAGAGA 571 329 736 Antisense
AAACCAATCTGATCCGGTCTGTGCT 338 125 812 Antisense
CCTACACCGTGCTTGCTTTTGATGA 132 267 839 Antisense
ATGAGCCCGACGCGGATTTTTCGAC 2 23 860 Antisense
Sequence Source Affymetrix Proprietary Database