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[186247_at]

Description H04M03.3 /REP_DB=WormBase Gene ID /WP=CE20922 /TR=Q9TYR0 /GB=AAD12788.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 36      6grp_high1_10_10 > 63      4grp_highest_8_8 > 31      4grp_high_8_8 > 05      6grp_highest_nonorm > 81      6grp_total > 63      4grp_total > 03      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 302
1 2 3 4 range
A 126.3
(-1.9)
P 		86.1
(-2.7)
P 		70.4
(-3)
P 		363.5
(3)
P 		293
B 120.9
(-2.1)
P 		90.8
(-2.7)
P 		74.6
(-3)
P 		371.6
(3)
P 		297
C 121
(-1.8)
P 		108.2
(-2.1)
P 		70.1
(-3)
P 		323.7
(3)
P 		254
avr 122.7
(-1.9) 		95
(-2.5) 		71.7
(-3) 		352.9
(3) 		281

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 325
1 2 3 4 5 6 range
A 112
(-3)
P 		301.5
(2)
P 		339.3
(3)
P 		280.8
(1.5)
P 		275.7
(1.3)
P 		221.6
(-0.1)
P 		227
B 116
(-3)
P 		289.9
(0.4)
P 		423.2
(3)
P 		318.9
(1)
P 		254.2
(-0.3)
P 		244.1
(-0.5)
P 		307
C 98.6
(-3)
P 		278.7
(0.6)
P 		396.3
(3)
P 		340.2
(1.9)
P 		296
(1)
P 		271.5
(0.5)
P 		298
avr 108.9
(-3) 		290
(0.9) 		386.3
(3) 		313.3
(1.4) 		275.3
(0.6) 		245.7
(-0) 		277
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 46
(1.8)
P 		18.7
(-2.9)
P 		18.4
(-3)
P 		53.2
(3)
P 		35
B 38.5
(-0.9)
P 		22.4
(-2.5)
P 		17.3
(-3)
P 		79.2
(3)
P 		62
C 40
(0.1)
P 		31.1
(-1.7)
P 		24.4
(-3)
P 		54.6
(3)
P 		30
avr 41.5
(0) 		24.1
(-2.4) 		20
(-3) 		62.3
(3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 20.6
(-3)
P 		107.2
(3)
P 		73.9
(0.7)
P 		71.2
(0.5)
P 		83.2
(1.3)
P 		54
(-0.7)
P 		87
B 40.4
(-3)
P 		96.5
(2.4)
P 		102.8
(3)
P 		84.5
(1.2)
P 		103.3
(3)
P 		91
(1.8)
P 		63
C 36.6
(-3)
P 		85
(1.5)
P 		100.9
(3)
P 		68.4
(-0)
P 		95.8
(2.5)
P 		90.9
(2.1)
P 		64
avr 32.5
(-3) 		96.2
(3) 		92.5
(2.7) 		74.7
(1) 		94.1
(2.8) 		78.6
(1.3) 		64
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186247_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11139
Sequence Type Exemplar sequence
Representative Public ID H04M03.3 NCBI
Target Description H04M03.3 /REP_DB=WormBase Gene ID /WP=CE20922 /TR=Q9TYR0 /GB=AAD12788.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068484(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068484 NCBI Caenorhabditis elegans H04M03.3 (H04M03.3) mRNA, complete cds. 11/11 None
SNAP00000034802 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5891579:5893197:1 11/11 None
GENEFINDER00000034809 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5891579:5893197:1 11/11 None
H04M03.3 ENSEMBL cdna:known chromosome:CEL140:IV:5891574:5893296:1 gene:H04M03.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5891584-5893203(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H04M03.3
Entrez Gene 177359 Entrez gene
SwissProt Q9TYR0 EMBL-EBI
WormBase CE20922 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAD12788 Hypothetical protein H04M03.3 [Caenorhabditis elegans] ref|NP_500885.1| H04M03.3 [Caenorhabditis elegans] 0.0
blast AAD12788 Hypothetical protein H04M03.3 [Caenorhabditis elegans] ref|NP_500885.1| H04M03.3 [Caenorhabditis elegans] 1.0E-180
blast CAE72629 Hypothetical protein CBG19827 [Caenorhabditis briggsae] 1.0E-145
blast CAE72629 Hypothetical protein CBG19827 [Caenorhabditis briggsae] 1.0E-142
blast NP_001009912 retinol dehydrogenase 12, like [Danio rerio] gb|AAH78208.1| Retinol dehydrogenase 12, like [Danio rerio] 2.0E-14
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 7.1E-12
Sequence
Target Sequence 
>C. ELEGANS:186247_AT
agtcgacgggatgatcaacgctctcttcattttgttttcagcacttcgattcttgtcaaa
ttccacaacgccactccactcggcatccgcttcttcaatccggaaaaagtgaccgattgg
cagaaatctctagttccaacagatgtctcgggtgctggaaagtatgcaatttcgaagatt
ggtctggcaaccctctccacttcaatttctcaatgcaatcttccaaatatcactgcaaca
agtgttcatccgggaactgtctacacaaatattatgtcaaatctcccggctcgtcagcaa
ttctacattaaacttgctcgtccgttcacgacttctctcgccgatgctggtgccaatctt
gttcgtgccgctgagaatccattgcctgctggaatgttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTCGACGGGATGATCAACGCTCTC 639 59 514 Antisense
TGTTTTCAGCACTTCGATTCTTGTC 549 561 546 Antisense
GCATCCGCTTCTTCAATCCGGAAAA 461 307 596 Antisense
AGTTCCAACAGATGTCTCGGGTGCT 345 65 645 Antisense
TCGAAGATTGGTCTGGCAACCCTCT 666 599 685 Antisense
TCTCCACTTCAATTTCTCAATGCAA 609 609 707 Antisense
TTCATCCGGGAACTGTCTACACAAA 677 687 758 Antisense
AATATTATGTCAAATCTCCCGGCTC 560 173 781 Antisense
ATTCTACATTAAACTTGCTCGTCCG 389 11 813 Antisense
CGATGCTGGTGCCAATCTTGTTCGT 492 249 855 Antisense
GAATCCATTGCCTGCTGGAATGTTC 198 331 888 Antisense
Sequence Source Affymetrix Proprietary Database