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[186237_at]

Description C08F11.14 /REP_DB=WormBase Gene ID /WP=CE23529 /TR=Q9U3Q5 /GB=CAB62784.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 0      4grp_total > 17      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 103
1 2 3 4 range
A 108.2
(-3)
A 		174.9
(3)
A 		142.5
(0.1)
A 		165.6
(2.2)
A 		67
B 91.4
(-1.3)
A 		71.8
(-3)
A 		86.4
(-1.8)
A 		142.8
(3)
A 		71
C 118.2
(-0.1)
A 		110.5
(-3)
A 		115.2
(-1.3)
A 		126.7
(3)
A 		16
avr 105.9
(-3) 		119.1
(-1) 		114.7
(-1.6) 		145
(3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 140
1 2 3 4 5 6 range
A 46
(-3)
A 		97.8
(0.8)
A 		126.9
(3)
A 		107.7
(1.6)
A 		76.1
(-0.8)
A 		94
(0.6)
A 		81
B 122.2
(2.3)
A 		107.3
(1.4)
A 		31.6
(-3)
A 		46.5
(-2.1)
A 		134.9
(3)
A 		113.4
(1.8)
A 		103
C 102.4
(-0.3)
A 		171.7
(3)
A 		45.3
(-3)
A 		129.7
(1)
A 		81.6
(-1.3)
A 		136.4
(1.3)
A 		126
avr 90.2
(-0.7) 		125.6
(3) 		67.9
(-3) 		94.6
(-0.2) 		97.5
(0.1) 		114.6
(1.9) 		58
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 39.4
(3)
A 		38
(2.4)
A 		37.2
(2.1)
A 		24.2
(-3)
A 		15
B 29.2
(2.4)
A 		17.7
(-3)
A 		20
(-1.9)
A 		30.4
(3)
A 		13
C 39.1
(2.6)
A 		31.8
(0.3)
A 		40.2
(3)
A 		21.4
(-3)
A 		19
avr 35.9
(3) 		29.2
(-0.8) 		32.5
(1.1) 		25.3
(-3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 8.5
(-3)
A 		34.8
(3)
A 		27.6
(1.4)
A 		27.3
(1.3)
A 		23
(0.3)
A 		22.9
(0.3)
A 		26
B 42.5
(1.4)
A 		35.7
(0.6)
A 		7.7
(-3)
A 		12.3
(-2.4)
A 		54.8
(3)
A 		42.3
(1.4)
A 		47
C 37.9
(0.9)
A 		52.4
(3)
A 		11.5
(-3)
A 		26.1
(-0.9)
A 		26.4
(-0.8)
A 		45.6
(2)
A 		41
avr 29.6
(0.3) 		41
(3) 		15.6
(-3) 		21.9
(-1.5) 		34.7
(1.5) 		36.9
(2) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186237_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9246
Sequence Type Exemplar sequence
Representative Public ID C08F11.14 NCBI
Target Description C08F11.14 /REP_DB=WormBase Gene ID /WP=CE23529 /TR=Q9U3Q5 /GB=CAB62784.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070225(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070225 NCBI Caenorhabditis elegans C08F11.14 (C08F11.14) mRNA, complete cds. 11/11 None
SNAP00000042595 ENSEMBL cdna:SNAP chromosome:CEL140:IV:13612776:13618030:-1 11/11 None
GENEFINDER00000042611 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13612776:13618030:-1 11/11 None
C08F11.14 ENSEMBL cdna:known chromosome:CEL140:IV:13612776:13618049:-1 gene:C08F11.14 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13612781-13617381(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C08F11.14
Entrez Gene 178328 Entrez gene
SwissProt Q9U3Q5 EMBL-EBI
WormBase CE33756 Wormbase
Functional Annotations
Ortholog
ID Title Organism
YEAST_2:1775729_AT Malate synthase, enzyme of the glyoxylate cycle, involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth in oleic acid medium Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6097 glyoxylate cycle inferred from electronic annotation QuickGO AmiGO
6099 tricarboxylic acid cycle inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
4451 isocitrate lyase activity inferred from electronic annotation QuickGO AmiGO
4474 malate synthase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB62784 Hypothetical protein C08F11.14 [Caenorhabditis elegans] ref|NP_502626.2| C08F11.14 [Caenorhabditis elegans] 0.0
blast AAB71278 Gex interacting protein protein 7, isoform a [Caenorhabditis elegans] ref|NP_503306.1| GEX Interacting protein family member (gei-7) [Caenorhabditis elegans] sp|Q10663|GCP_CAEEL Bifunctional glyoxylate cycle protein (GEX interacting protein 7) [Includes: Isocitrate lyase (Isocitrase) (Isocitratase) (ICL); Malate synthase ] 0.0
blast CAE62276 Hypothetical protein CBG06335 [Caenorhabditis briggsae] 0.0
blast AAA85857 bifunctional glyoxylate cycle protein 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000918 EMBL-EBI Isocitrate lyase and phosphorylmutase 1.0E-126
Pfam IPR000918 EMBL-EBI Isocitrate lyase and phosphorylmutase 7.6E-83
Pfam IPR000918 EMBL-EBI Isocitrate lyase and phosphorylmutase 1.7E-27
Pfam IPR001465 EMBL-EBI Malate synthase 1.0E-126
Pfam IPR000918 EMBL-EBI Isocitrate lyase and phosphorylmutase 3.5E-63
Pfam IPR000918 EMBL-EBI Isocitrate lyase and phosphorylmutase 1.1E-30
Pfam IPR001465 EMBL-EBI Malate synthase 1.0E-126
Pfam IPR001465 EMBL-EBI Malate synthase 1.0E-126
Pfam IPR001465 EMBL-EBI Malate synthase 1.0E-126
Pfam IPR001465 EMBL-EBI Malate synthase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:186237_AT
atggctgcacacattccaatcaaacatgatcaaattgcaaatgacaaggcttttgctctt
gtcacaagtgataaacaacgagaggttactgatggacatgacggaacatggattgctcat
ccgggattggttccagtggctcagcgagtttttaatgagatgatgccaaggcctaatcag
attgacaagttgctcaatcgtccaatagctacaaaagagcaattgacaatggttccagag
ggtacacgtaccgatcttggattcagaaataatatttctgttactttgggatacctagac
tattggctccgcggagtcggatgtgtgccacttgacaatttaatggaagatctcgcaacg
gctgaaatctcacgtgctcaactttggcaatggcttcatcatgaggcaaagcttgaagat
ggacgtgttattgatacaggacttgtaaaacaaacaattgccgccgaagctgaacaccga
tcgattcgtgctggttcagttgtgaatcgtattccagaagccgccgagcttctcgagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGCTGCACACATTCCAATCAAAC 170 53 2122 Antisense
GGATTGCTCATCCGGGATTGGTTCC 111 507 2231 Antisense
ATTGGTTCCAGTGGCTCAGCGAGTT 705 3 2247 Antisense
GGTACACGTACCGATCTTGGATTCA 269 505 2362 Antisense
GGATACCTAGACTATTGGCTCCGCG 439 509 2410 Antisense
GAGTCGGATGTGTGCCACTTGACAA 284 401 2435 Antisense
GATCTCGCAACGGCTGAAATCTCAC 317 419 2470 Antisense
AAATCTCACGTGCTCAACTTTGGCA 120 119 2486 Antisense
AACAATTGCCGCCGAAGCTGAACAC 621 133 2574 Antisense
AACACCGATCGATTCGTGCTGGTTC 285 135 2594 Antisense
CAGAAGCCGCCGAGCTTCTCGAGAA 14 205 2636 Antisense
Sequence Source Affymetrix Proprietary Database