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[186235_s_at]

Description R10D12.12 /REP_DB=WormBase Gene ID /WP=CE23919 /TR=P92012 /GB=CAB03253.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 57      6grp_high1_10_10 > 85      4grp_highest_8_8 > 37      4grp_high_8_8 > 03      6grp_highest_nonorm > 73      6grp_total > 75      4grp_total > 06      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 226
1 2 3 4 range
A 418.7
(-0.3)
P 		315.6
(-3)
P 		438.2
(0.2)
P 		542
(3)
P 		226
B 473
(3)
P 		384.9
(-3)
P 		395
(-2.3)
P 		400.8
(-1.9)
P 		88
C 424.4
(-0.2)
P 		426.7
(-0.1)
P 		360.3
(-3)
P 		496.4
(3)
P 		136
avr 438.7
(0.6) 		375.7
(-3) 		397.8
(-1.7) 		479.7
(3) 		104

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 533
1 2 3 4 5 6 range
A 324.3
(-3)
P 		497
(-0.2)
P 		696.9
(2.9)
P 		647.4
(2.1)
P 		516.9
(0.1)
P 		700.8
(3)
P 		377
B 444.5
(-3)
P 		500.1
(-2.2)
P 		668.5
(0.3)
P 		600.9
(-0.7)
P 		514.8
(-2)
P 		857.5
(3)
P 		413
C 393.1
(-3)
P 		485.6
(-1.4)
P 		586.8
(0.3)
P 		633
(1.1)
P 		567.6
(-0)
P 		746.8
(3)
P 		354
avr 387.3
(-3) 		494.2
(-1.3) 		650.7
(1.1) 		627.1
(0.8) 		533.1
(-0.7) 		768.4
(3) 		381
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 152.4
(3)
P 		68.5
(-3)
P 		114.6
(0.3)
P 		79.3
(-2.2)
P 		84
B 150.8
(3)
P 		95.1
(-2.1)
P 		91.5
(-2.4)
P 		85.4
(-3)
P 		65
C 140.5
(3)
P 		122.7
(1.1)
P 		125.6
(1.4)
P 		83.7
(-3)
P 		57
avr 147.9
(3) 		95.4
(-1.8) 		110.6
(-0.4) 		82.8
(-3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 260
1 2 3 4 5 6 range
A 59.8
(-3)
P 		176.7
(3)
P 		151.8
(1.7)
P 		164.2
(2.4)
P 		156
(1.9)
P 		170.6
(2.7)
P 		117
B 154.7
(-3)
P 		166.5
(-2.6)
P 		162.3
(-2.7)
P 		159.2
(-2.8)
P 		209.2
(-1)
P 		319.8
(3)
P 		165
C 145.7
(-2.1)
P 		148.2
(-2)
P 		149.3
(-1.9)
P 		127.3
(-3)
P 		183.7
(-0.2)
P 		250
(3)
P 		123
avr 120.1
(-3) 		163.8
(-0.9) 		154.5
(-1.4) 		150.2
(-1.6) 		183
(-0) 		246.8
(3) 		127
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186235_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13592
Sequence Type Exemplar sequence
Representative Public ID R10D12.12 NCBI
Target Description R10D12.12 /REP_DB=WormBase Gene ID /WP=CE23919 /TR=P92012 /GB=CAB03253.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074066(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074066 NCBI Caenorhabditis elegans R10D12.12 (R10D12.12) mRNA, complete cds. 11/11 None
SNAP00000021056 ENSEMBL cdna:SNAP chromosome:CEL140:V:13963912:13964543:-1 11/11 None
GENEFINDER00000021073 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13961101:13964543:-1 11/11 None
R10D12.12 ENSEMBL cdna:known chromosome:CEL140:V:13963903:13964565:-1 gene:R10D12.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13963911-13964543(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R10D12.12
Entrez Gene 179890 Entrez gene
SwissProt P92012 EMBL-EBI
WormBase CE23919 Wormbase
Functional Annotations
Ortholog
ID Title Organism
MG-U74CV2:167376_AT glycosyltransferase 28 domain containing 2 mm
MOE430B:1455455_AT glycosyltransferase 28 domain containing 2 mm
MOUSE430_2:1455455_AT glycosyltransferase 28 domain containing 2 mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
30259 lipid glycosylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16758 transferase activity, transferring hexosyl groups inferred from electronic annotation QuickGO AmiGO
30246 carbohydrate binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03254 Hypothetical protein R10D12.11 [Caenorhabditis elegans] ref|NP_506466.2| Serpentine Receptor, class H family member (srh-24) [Caenorhabditis elegans] 1.0E-122
blast CAE66181 Hypothetical protein CBG11419 [Caenorhabditis briggsae] 1.0E-100
blast CAB03253 Hypothetical protein R10D12.12 [Caenorhabditis elegans] ref|NP_506467.1| R10D12.12 [Caenorhabditis elegans] 3.0E-98
blast CAB03253 Hypothetical protein R10D12.12 [Caenorhabditis elegans] ref|NP_506467.1| R10D12.12 [Caenorhabditis elegans] 2.0E-88
blast CAE66180 Hypothetical protein CBG11418 [Caenorhabditis briggsae] 1.0E-79
blast AAH76360 Zgc:92907 [Danio rerio] ref|NP_001002460.1| hypothetical protein LOC436733 [Danio rerio] 2.0E-29
Protein Domains
Database ID Description E-Value
Pfam IPR007235 EMBL-EBI Glycosyltransferase 28, C-terminal 1.7E-50
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.2E-72
Pfam IPR007235 EMBL-EBI Glycosyltransferase 28, C-terminal 8.4E-47
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000021073 5 165-187,202-224,245-264,300-322,346-368
Sequence
Target Sequence 
>C. ELEGANS:186235_S_AT
gagttgcttcgtgacggttggatcaacattgttcgaagacttgataaatcaagttctttg
tgaagcatcactagaaaatctcaaaaagatcggagtcaagaagattcgccttcaaattgg
aaaaggaaatttcaatcaagacgtcattgatcgtgtttttggagaaacatctggagatga
aggatctgtaaagtgtgatggtctcgatatagattattatcgttacaagccatcgttgag
tgaagatatggctgaagctttgattgtaattggccacggaggtgccggaacttgcttgga
agtgttggcgcttcatctcccattcatcacagtaacaaacgataaactaatggacaatca
tcaagccgaactcgccgtgcaactttcagacgaaggataccttctccaatgcaccccatc
aacgcttccagaaactattttgaaagaaaatttgttctctcttagacaatttgcagcgcc
ttcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGTTGCTTCGTGACGGTTGGATCA 707 391 15 Antisense
TTATCGTTACAAGCCATCGTTGAGT 136 679 231 Antisense
GTAATTGGCCACGGAGGTGCCGGAA 275 457 280 Antisense
GAGGTGCCGGAACTTGCTTGGAAGT 619 401 293 Antisense
TTGGAAGTGTTGGCGCTTCATCTCC 314 709 310 Antisense
CATCTCCCATTCATCACAGTAACAA 66 213 328 Antisense
GGACAATCATCAAGCCGAACTCGCC 184 527 366 Antisense
CTCGCCGTGCAACTTTCAGACGAAG 700 233 385 Antisense
GACGAAGGATACCTTCTCCAATGCA 675 379 403 Antisense
CCCCATCAACGCTTCCAGAAACTAT 70 263 428 Antisense
CTTAGACAATTTGCAGCGCCTTCAA 436 219 475 Antisense
Sequence Source Affymetrix Proprietary Database