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[186225_at]

Description F09D12.2 /REP_DB=WormBase Gene ID /WP=CE16972 /TR=O44472 /GB=AAB92034.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 34      4grp_total > 24      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 75.4
(-3)
A 		76.6
(-2.8)
A 		111.6
(2.8)
A 		113.1
(3)
A 		38
B 64.3
(-3)
A 		71.5
(2.2)
A 		67.2
(-0.9)
A 		72.6
(3)
A 		8
C 49.3
(-3)
A 		50.4
(-2.8)
A 		76
(1.6)
A 		84.3
(3)
A 		35
avr 63
(-3) 		66.2
(-2.3) 		84.9
(1.9) 		90
(3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 82
1 2 3 4 5 6 range
A 99.3
(3)
A 		39.9
(-1.6)
A 		77.2
(1.3)
A 		59
(-0.1)
A 		47
(-1)
A 		21.5
(-3)
A 		78
B 41.4
(-0.4)
A 		72.2
(3)
A 		66.6
(2.4)
A 		17.1
(-3)
A 		53.3
(0.9)
A 		30.3
(-1.6)
A 		55
C 38.4
(0.4)
A 		44.4
(1.4)
A 		20
(-2.7)
A 		18.1
(-3)
A 		28.6
(-1.3)
A 		54.1
(3)
A 		36
avr 59.7
(3) 		52.2
(1.4) 		54.6
(1.9) 		31.4
(-3) 		43
(-0.5) 		35.3
(-2.2) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 27.4
(2.1)
A 		16.6
(-3)
A 		29.2
(3)
A 		16.6
(-3)
A 		13
B 20.5
(3)
A 		17.7
(-0.4)
A 		15.6
(-2.9)
A 		15.5
(-3)
A 		5
C 16.3
(-2)
A 		14.5
(-2.9)
A 		26.5
(3)
A 		14.2
(-3)
A 		12
avr 21.4
(1.3) 		16.3
(-2.4) 		23.8
(3) 		15.4
(-3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 18.3
(3)
A 		14.2
(1.1)
A 		16.8
(2.3)
A 		15
(1.5)
A 		14.2
(1.1)
A 		5.2
(-3)
A 		13
B 14.4
(0)
A 		24
(3)
A 		16.2
(0.6)
A 		4.5
(-3)
A 		21.7
(2.3)
A 		11.3
(-0.9)
A 		20
C 14.2
(1.4)
A 		13.5
(1.1)
A 		5.1
(-2.4)
A 		3.6
(-3)
A 		9.3
(-0.6)
A 		18.1
(3)
A 		15
avr 15.6
(2) 		17.2
(3) 		12.7
(0.2) 		7.7
(-3) 		15.1
(1.7) 		11.5
(-0.6) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186225_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10481
Sequence Type Exemplar sequence
Representative Public ID F09D12.2 NCBI
Target Description F09D12.2 /REP_DB=WormBase Gene ID /WP=CE16972 /TR=O44472 /GB=AAB92034.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067947(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067947 NCBI Caenorhabditis elegans F09D12.2 (F09D12.2) mRNA, complete cds. 11/11 None
GENEFINDER00000007130 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3442300:3444019:-1 11/11 None
F09D12.2 ENSEMBL cdna:known chromosome:CEL140:IV:3442300:3444198:-1 gene:F09D12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3442305-3444204(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F09D12.2
Entrez Gene 184240 Entrez gene
SwissProt O44472 EMBL-EBI
WormBase CE16972 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB92034 Hypothetical protein F09D12.2 [Caenorhabditis elegans] ref|NP_500348.1| F09D12.2 [Caenorhabditis elegans] 0.0
blast AAF59425 Hypothetical protein Y19D10B.6 [Caenorhabditis elegans] ref|NP_503636.1| Y19D10B.6 [Caenorhabditis elegans] 1.0E-156
blast NP_503898 T28A11.17 [Caenorhabditis elegans] gb|AAC48126.2| Hypothetical protein T28A11.17 [Caenorhabditis elegans] 1.0E-155
blast AAF59425 Hypothetical protein Y19D10B.6 [Caenorhabditis elegans] ref|NP_503636.1| Y19D10B.6 [Caenorhabditis elegans] 1.0E-139
blast NP_503898 T28A11.17 [Caenorhabditis elegans] gb|AAC48126.2| Hypothetical protein T28A11.17 [Caenorhabditis elegans] 1.0E-136
Protein Domains
Database ID Description E-Value
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 2.0E-82
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 2.0E-82
Sequence
Target Sequence 
>C. ELEGANS:186225_AT
aacttcttgtttcgatcgcttttattgaatcttgaaagtgacgcagctcagtacattagt
ttgattcatgttacagtagtcacgagaagtgatcttcagcagccagttaaagcgatagcc
gataaacttttcgtacttgctcatgagcttatgcattgggtatacccacaaggaacatgg
ctgatggattcaattgttactagaacagctgaaaaatgtgcacgagacgaggtgaagatg
atgggggacactgatgcagtgaagccagttgatggatggtttagcgaagaagttactcat
gaagatctggcgaatataatggctatgaggatggttatgaagatggcggccaggaaatct
gcaaatgaaaagcagctctccctggaaacaatcatgagtagtttgtgcgtccaaggcaaa
ccgaaaaacaccgtatgccgcaatcatcatccctttgagctttctctcaacaccgctgtt
cgtcaatatccactattcagctcactttacggatgtcgtgccggtgatcggatgtttgct
aagcgggaagagttctgtaagccgctgggtggcgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACTTCTTGTTTCGATCGCTTTTAT 373 139 760 Antisense
GAAAGTGACGCAGCTCAGTACATTA 188 361 793 Antisense
GAAGTGATCTTCAGCAGCCAGTTAA 654 335 845 Antisense
AACTTTTCGTACTTGCTCATGAGCT 439 139 884 Antisense
AAAGCAGCTCTCCCTGGAAACAATC 283 123 1128 Antisense
AAACACCGTATGCCGCAATCATCAT 406 129 1185 Antisense
TCTCAACACCGCTGTTCGTCAATAT 536 611 1224 Antisense
ATATCCACTATTCAGCTCACTTTAC 500 21 1245 Antisense
GCTCACTTTACGGATGTCGTGCCGG 513 301 1259 Antisense
GTCGTGCCGGTGATCGGATGTTTGC 410 473 1274 Antisense
GTTCTGTAAGCCGCTGGGTGGCGAG 642 439 1311 Antisense
Sequence Source Affymetrix Proprietary Database