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[186223_at]

Description F33G12.4 /REP_DB=WormBase Gene ID /WP=CE27150 /TR=Q19988 /GB=AAK31512.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 98      4grp_high_8_8 > 13      6grp_highest_nonorm > 94      6grp_total > 97      4grp_total > 11      6grp_highest_sub > Da      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 113
1 2 3 4 range
A 248.8
(-2.8)
P 		285.8
(3)
P 		254.5
(-1.9)
P 		247.6
(-3)
M 		38
B 273.7
(-0.3)
P 		244.2
(-1.8)
P 		221.9
(-3)
P 		335.2
(3)
P 		113
C 241.3
(-1.5)
P 		222.1
(-3)
P 		243.6
(-1.4)
P 		300.8
(3)
P 		79
avr 254.6
(-1.4) 		250.7
(-1.8) 		240
(-3) 		294.5
(3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 679
1 2 3 4 5 6 range
A 294.7
(-2.9)
P 		283.6
(-3)
P 		303
(-2.8)
P 		391
(-1.8)
P 		717.8
(1.7)
P 		833
(3)
P 		549
B 268.1
(-3)
P 		274.5
(-2.9)
P 		310.4
(-2.6)
P 		385
(-1.9)
P 		778.3
(1.8)
P 		909.5
(3)
P 		641
C 235.2
(-3)
P 		294.7
(-2.5)
P 		286.7
(-2.5)
P 		361.7
(-1.9)
P 		609.2
(0.3)
P 		913.8
(3)
P 		679
avr 266
(-3) 		284.3
(-2.8) 		300
(-2.7) 		379.2
(-1.9) 		701.8
(1.2) 		885.4
(3) 		619
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 90.6
(3)
P 		62
(-0.2)
P 		66.5
(0.3)
P 		36.2
(-3)
M 		54
B 87.3
(3)
P 		60.3
(-1.5)
P 		51.4
(-3)
P 		71.4
(0.3)
P 		36
C 79.9
(2.1)
P 		63.9
(-0.7)
P 		84.9
(3)
P 		50.7
(-3)
P 		34
avr 85.9
(3) 		62.1
(-1.3) 		67.6
(-0.3) 		52.8
(-3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 285
1 2 3 4 5 6 range
A 54.3
(-3)
P 		100.8
(-1.3)
P 		66
(-2.6)
P 		99.2
(-1.3)
P 		216.6
(3)
P 		202.8
(2.5)
P 		162
B 93.3
(-2.6)
P 		91.4
(-2.6)
P 		75.4
(-3)
P 		102
(-2.4)
P 		316.2
(2.5)
P 		339.2
(3)
P 		264
C 87.2
(-2.6)
P 		89.9
(-2.6)
P 		73
(-3)
P 		72.7
(-3)
P 		197.1
(0.2)
P 		305.9
(3)
P 		233
avr 78.3
(-2.8) 		94
(-2.4) 		71.5
(-3) 		91.3
(-2.4) 		243.3
(1.9) 		282.6
(3) 		211
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186223_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5442
Sequence Type Exemplar sequence
Representative Public ID F33G12.4 NCBI
Target Description F33G12.4 /REP_DB=WormBase Gene ID /WP=CE27150 /TR=Q19988 /GB=AAK31512.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062527(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062527 NCBI Caenorhabditis elegans F33G12.4 (F33G12.4) mRNA, complete cds. 11/11 A 
SNAP00000025575 ENSEMBL cdna:SNAP chromosome:CEL140:II:5034279:5036410:-1 11/11 None
GENEFINDER00000025584 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5034245:5036410:-1 11/11 None
F33G12.4.3 ENSEMBL cdna:known chromosome:CEL140:II:5033794:5036423:-1 gene:F33G12.4 11/11 A 
F33G12.4.2 ENSEMBL cdna:known chromosome:CEL140:II:5033816:5036423:-1 gene:F33G12.4 11/11 A 
F33G12.4.1 ENSEMBL cdna:known chromosome:CEL140:II:5034128:5036423:-1 gene:F33G12.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5034242-5036408(-) 97.84 100.0
Public Domain and Genome References
Gene Title
Ensembl F33G12.4
Entrez Gene 173869 Entrez gene
SwissProt Q19988 EMBL-EBI
WormBase CE27150 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK31512 Hypothetical protein F33G12.4 [Caenorhabditis elegans] ref|NP_494928.1| F33G12.4 [Caenorhabditis elegans] 0.0
blast T16243 hypothetical protein F33G12.4 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.033
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.22
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.64
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.11
Sequence
Target Sequence 
>C. ELEGANS:186223_AT
gcaacaatcttatgacagctttaccttatgtattctcaaaaattccaagtatcgaacatg
tgggaattaatgccaatgatcaaatggttagattagaagaagaaattggaccaattttca
tacgtcgcctgcttacaaaagttgataatatcgaatacacacacgagggaggtgtcgaca
aattgggcacaattgctgcgaatgctattcatcgagaagaatgctcaaaatcgatgaaat
ttggacgtcaattccttccactctcacttcgtttcgatatggatgatctgatgtttgaaa
aaccaggaatatcatctctcgaattgtgtacaggatgtgtcagacttcggcatatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAACAATCTTATGACAGCTTTACC 105 319 839 Antisense
GACAGCTTTACCTTATGTATTCTCA 278 369 852 Antisense
TGGACCAATTTTCATACGTCGCCTG 156 547 945 Antisense
CATACGTCGCCTGCTTACAAAAGTT 611 213 957 Antisense
GGGAGGTGTCGACAAATTGGGCACA 519 495 1005 Antisense
GGGCACAATTGCTGCGAATGCTATT 290 493 1023 Antisense
TGCTGCGAATGCTATTCATCGAGAA 658 579 1032 Antisense
AATTTGGACGTCAATTCCTTCCACT 494 173 1076 Antisense
TCCACTCTCACTTCGTTTCGATATG 241 589 1095 Antisense
AAACCAGGAATATCATCTCTCGAAT 601 125 1138 Antisense
AGGATGTGTCAGACTTCGGCATATT 148 55 1170 Antisense
Sequence Source Affymetrix Proprietary Database