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[186220_at]

Description R03H10.2 /REP_DB=WormBase Gene ID /WP=CE25965 /TR=Q9GYM4 /GB=AAG00006.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 01      6grp_highest_nonorm > 40      6grp_total > 23      4grp_total > 52      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 675
1 2 3 4 range
A 660.4
(1.9)
P 		778
(3)
P 		629.7
(1.6)
P 		145.4
(-3)
A 		633
B 673.4
(1.9)
P 		795.1
(3)
P 		629.7
(1.5)
P 		146.9
(-3)
A 		648
C 777.2
(2.6)
P 		820.7
(3)
P 		534.7
(0.2)
P 		197.3
(-3)
A 		623
avr 703.7
(2.1) 		797.9
(3) 		598
(1.1) 		163.2
(-3) 		635

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1031
1 2 3 4 5 6 range
A 1128.1
(3)
P 		651.2
(0)
P 		660.5
(0.1)
P 		489.9
(-1)
P 		324.6
(-2)
P 		173.3
(-3)
P 		955
B 1204.1
(3)
P 		738.4
(0.3)
P 		835.1
(0.8)
P 		618.9
(-0.5)
P 		275.6
(-2.5)
P 		187
(-3)
P 		1017
C 911.3
(3)
P 		731.8
(1.4)
P 		628
(0.5)
P 		457.4
(-1)
P 		359.5
(-1.9)
P 		234.3
(-3)
P 		677
avr 1081.2
(3) 		707.1
(0.5) 		707.9
(0.5) 		522.1
(-0.8) 		319.9
(-2.2) 		198.2
(-3) 		883
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 236
1 2 3 4 range
A 240.4
(3)
P 		168.9
(1)
P 		164.6
(0.9)
P 		21.3
(-3)
A 		219
B 214.7
(3)
P 		196.4
(2.4)
P 		145.9
(0.7)
P 		31.3
(-3)
A 		183
C 257.3
(3)
P 		236
(2.4)
P 		186.3
(1.1)
P 		33.3
(-3)
A 		224
avr 237.5
(3) 		200.4
(1.9) 		165.6
(0.9) 		28.6
(-3) 		209

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 377
1 2 3 4 5 6 range
A 208
(2.3)
P 		231.6
(3)
P 		143.8
(0.2)
P 		124.2
(-0.4)
P 		98
(-1.2)
P 		42.2
(-3)
P 		189
B 419
(3)
P 		245.8
(0)
P 		202.8
(-0.7)
P 		163.9
(-1.4)
P 		112
(-2.3)
P 		69.7
(-3)
P 		349
C 337.8
(3)
P 		223.3
(0.4)
P 		159.8
(-1.1)
P 		92
(-2.7)
P 		116.3
(-2.1)
P 		78.4
(-3)
P 		259
avr 321.6
(3) 		233.6
(1) 		168.8
(-0.6) 		126.7
(-1.5) 		108.8
(-1.9) 		63.4
(-3) 		258
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186220_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4455
Sequence Type Exemplar sequence
Representative Public ID R03H10.2 NCBI
Target Description R03H10.2 /REP_DB=WormBase Gene ID /WP=CE25965 /TR=Q9GYM4 /GB=AAG00006.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062327(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062327 NCBI Caenorhabditis elegans R03H10.2 (R03H10.2) mRNA, complete cds. 11/11 None
GENEFINDER00000041951 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4155769:4167131:1 11/11 None
R03H10.2 ENSEMBL cdna:known chromosome:CEL140:II:4155766:4167298:1 gene:R03H10.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000041937 3/11 Cross Hyb Matching Probes None
SNAP00000041939 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4155767-4167130(+) 99.56 100.0
Public Domain and Genome References
Gene Title
Ensembl R03H10.2
Entrez Gene 173751 Entrez gene
SwissProt Q9GYM4 EMBL-EBI
WormBase CE25965 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG00006 Hypothetical protein R03H10.2 [Caenorhabditis elegans] ref|NP_494728.1| R03H10.2 [Caenorhabditis elegans] 1.0E-177
blast AAG00006 Hypothetical protein R03H10.2 [Caenorhabditis elegans] ref|NP_494728.1| R03H10.2 [Caenorhabditis elegans] 1.0E-175
blast CAE74275 Hypothetical protein CBG21967 [Caenorhabditis briggsae] 1.0E-165
blast CAE74275 Hypothetical protein CBG21967 [Caenorhabditis briggsae] 1.0E-164
blast T16669 hypothetical protein R03H10.2 - Caenorhabditis elegans 6.0E-72
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494728.1 4 44-66,119-141,151-173,237-259
R03H10.2 4 44-66,119-141,151-173,237-259
GENEFINDER00000041951 3 160-182,191-213,281-303
Sequence
Target Sequence 
>C. ELEGANS:186220_AT
tgccctactcaaacatgcttccaaacgccaccgagaaaattctgaaccacgcgattccgc
ataatgtgggtggcattcaacaaatggaaattggactcggctgcacatttgacttgaatt
tatatgcggcaaatcggagaaaacttaatcccaataacacttgcgatccacaaatcgact
cttctttcatcccaatctacatgttaattggtctccttgtcattcgcctgctcccaattg
tgctcggtgtcctcctcgccgttaaaaagaccccgcttagcgaaggagtcgcacaacttg
ttgagaaacttcggaaaggggaaaagagaaggttatacgttaagaagcaacaacgaacca
cgaccacttttgatggagagcatgtgagcaagagtacgacttttggagttgatggtactc
caccaagaatcacgtcaactccacttccgcctgttccatcgccagctcaaattgatcata
gcattagctacaacaacgccgcctattttgccacttctggcgctttttcctctcgatcca
gtgatatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCCCTACTCAAACATGCTTCCAAA 323 585 536 Antisense
ATTCTGAACCACGCGATTCCGCATA 239 9 574 Antisense
ACTCGGCTGCACATTTGACTTGAAT 287 99 630 Antisense
TGCGATCCACAAATCGACTCTTCTT 402 583 697 Antisense
CTTCTTTCATCCCAATCTACATGTT 514 221 716 Antisense
ATGTTAATTGGTCTCCTTGTCATTC 598 47 736 Antisense
GACCCCGCTTAGCGAAGGAGTCGCA 207 379 804 Antisense
CAACGAACCACGACCACTTTTGATG 691 187 886 Antisense
GTACTCCACCAAGAATCACGTCAAC 303 459 950 Antisense
TTCCATCGCCAGCTCAAATTGATCA 432 699 989 Antisense
TTTTTCCTCTCGATCCAGTGATATT 499 673 1059 Antisense
Sequence Source Affymetrix Proprietary Database