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[186215_at]

Description F31F4.15 /REP_DB=WormBase Gene ID /WP=CE09835 /TR=O17135 /GB=AAG24083.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 72      4grp_high_8_8 > 55      6grp_total > 70      4grp_total > 48      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 374
1 2 3 4 range
A 35.2
(-3)
P 		179.4
(-0.4)
P 		363.1
(3)
P 		81
(-2.2)
P 		328
B 4.8
(-3)
A 		256.7
(2.8)
P 		264.1
(3)
P 		74.2
(-1.4)
P 		259
C 51.2
(-3)
A 		228.9
(0.3)
P 		379
(3)
P 		77.4
(-2.5)
A 		328
avr 30.4
(-3) 		221.7
(0.8) 		335.4
(3) 		77.5
(-2.1) 		305

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 255
1 2 3 4 5 6 range
A 69.8
(-2.2)
P 		45.4
(-3)
P 		153.8
(0.7)
P 		219.8
(3)
P 		95.9
(-1.3)
P 		59.2
(-2.5)
P 		174
B 40.5
(-3)
P 		69.2
(-1.4)
P 		150
(2.9)
P 		151.2
(3)
P 		81.1
(-0.8)
P 		46.4
(-2.7)
P 		111
C 45.7
(-3)
A 		47.8
(-2.9)
P 		94
(-1.8)
P 		295.3
(3)
P 		68.9
(-2.4)
P 		89.9
(-1.9)
P 		250
avr 52
(-3) 		54.1
(-2.9) 		132.6
(-0.2) 		222.1
(3) 		82
(-1.9) 		65.2
(-2.5) 		170
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 131
1 2 3 4 range
A 12.8
(-2.9)
P 		39
(-1)
P 		94.9
(3)
P 		11.9
(-3)
P 		83
B 1.5
(-3)
A 		63.4
(3)
P 		61.2
(2.8)
P 		15.8
(-1.6)
P 		62
C 16.9
(-2.8)
A 		65.8
(-0.3)
P 		132.1
(3)
P 		13.1
(-3)
A 		119
avr 10.4
(-3) 		56.1
(0.2) 		96.1
(3) 		13.6
(-2.8) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 12.9
(-3)
P 		16.1
(-2.6)
P 		33.5
(-0.1)
P 		55.8
(3)
P 		28.9
(-0.8)
P 		14.4
(-2.8)
P 		43
B 14.1
(-3)
P 		23
(-0.9)
P 		36.4
(2.1)
P 		40.1
(3)
P 		33
(1.4)
P 		17.3
(-2.3)
P 		26
C 16.9
(-2.7)
A 		14.6
(-3)
P 		23.9
(-1.8)
P 		59.4
(3)
P 		22.3
(-2)
P 		30.1
(-0.9)
P 		45
avr 14.6
(-3) 		17.9
(-2.5) 		31.3
(-0.3) 		51.8
(3) 		28.1
(-0.8) 		20.6
(-2) 		37
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186215_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15645
Sequence Type Exemplar sequence
Representative Public ID F31F4.15 NCBI
Target Description F31F4.15 /REP_DB=WormBase Gene ID /WP=CE09835 /TR=O17135 /GB=AAG24083.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070889(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070889 NCBI Caenorhabditis elegans F31F4.15 (F31F4.15) mRNA, complete cds. 11/11 None
SNAP00000012368 ENSEMBL cdna:SNAP chromosome:CEL140:V:672075:675704:-1 11/11 None
GENEFINDER00000012375 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:672075:676316:-1 11/11 None
F31F4.15 ENSEMBL cdna:known chromosome:CEL140:V:674833:676583:-1 gene:F31F4.15 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:674832-676583(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F31F4.15
Entrez Gene 185173 Entrez gene
SwissProt O17135 EMBL-EBI
WormBase CE09835 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503290 F31F4.15 [Caenorhabditis elegans] gb|AAG24083.1| Hypothetical protein F31F4.15 [Caenorhabditis elegans] 0.0
blast NP_503290 F31F4.15 [Caenorhabditis elegans] gb|AAG24083.1| Hypothetical protein F31F4.15 [Caenorhabditis elegans] 1.0E-166
blast NP_503290 F31F4.15 [Caenorhabditis elegans] gb|AAG24083.1| Hypothetical protein F31F4.15 [Caenorhabditis elegans] 1.0E-151
blast NP_503289 Serpentine Receptor, class U family member (sru-25) [Caenorhabditis elegans] gb|AAG24092.2| Serpentine receptor, class u protein 25 [Caenorhabditis elegans] 1.0E-151
blast NP_503287 Serpentine Receptor, class U family member (sru-24) [Caenorhabditis elegans] gb|AAG24091.2| Serpentine receptor, class u protein 24 [Caenorhabditis elegans] 3.0E-87
blast NP_503289 Serpentine Receptor, class U family member (sru-25) [Caenorhabditis elegans] gb|AAG24092.2| Serpentine receptor, class u protein 25 [Caenorhabditis elegans] 4.0E-49
blast AAC68938 Hypothetical protein T12B5.11 [Caenorhabditis elegans] ref|NP_497302.1| T12B5.11 [Caenorhabditis elegans] 7.0E-47
blast AAC68938 Hypothetical protein T12B5.11 [Caenorhabditis elegans] ref|NP_497302.1| T12B5.11 [Caenorhabditis elegans] 1.0E-46
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 1.0E-56
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 1.0E-56
Pfam IPR003839 EMBL-EBI Protein of unknown function DUF215 8.4E-72
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 1.0E-56
Pfam IPR003839 EMBL-EBI Protein of unknown function DUF215 3.6E-84
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503290.1 1 20-42
F31F4.15 1 20-42
SNAP00000012368 3 301-323,336-358,378-400
GENEFINDER00000012375 6 345-367,380-402,417-439,452-474,505-527,563-585
Sequence
Target Sequence 
>C. ELEGANS:186215_AT
cgaaggctatgatctttgaccgcttctcaataagggatactatttctatcctctcacgtg
tttatgcacaaacgctggaaaacattcacatgtggcacactgacattcgtgattttgatc
agtttgaacggatgattgaaatggatcaatggaaaaatgcgaaaaaatgcacaatttggg
aatcctcgtttgacaccaaatatacagaaaatttgttccattttgactcatttaccattt
accaaatgtcctccagcctaaaaacacagcttgctatcaaaattagagacgatgtaatga
ccagaagtacatttgaatattgtaacctacgttactacgaaacagtttacacgaattcac
tcgaaattgcgaaagtattcaagccagactatgaaggtggcgctgattttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGAAGGCTATGATCTTTGACCGCTT 440 253 557 Antisense
TGACCGCTTCTCAATAAGGGATACT 171 569 573 Antisense
GGGATACTATTTCTATCCTCTCACG 420 495 590 Antisense
TTCTATCCTCTCACGTGTTTATGCA 174 691 600 Antisense
GAAAACATTCACATGTGGCACACTG 677 353 634 Antisense
GTGGCACACTGACATTCGTGATTTT 527 491 648 Antisense
TGGGAATCCTCGTTTGACACCAAAT 324 553 733 Antisense
ATTTTGACTCATTTACCATTTACCA 201 15 776 Antisense
ATGTCCTCCAGCCTAAAAACACAGC 132 49 802 Antisense
GAAACAGTTTACACGAATTCACTCG 694 353 895 Antisense
GACTATGAAGGTGGCGCTGATTTTG 103 373 943 Antisense
Sequence Source Affymetrix Proprietary Database