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[186212_at]

Description F54D10.6 /REP_DB=WormBase Gene ID /WP=CE19456 /TR=Q9TZ86 /GB=AAC68786.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 47      4grp_high_8_8 > 20      6grp_total > 47      4grp_total > 21      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 119
1 2 3 4 range
A 71.2
(1.3)
P 		29.8
(-2.1)
A 		18.2
(-3)
A 		93
(3)
A 		75
B 117.1
(3)
P 		91.3
(1.1)
P 		33.8
(-3)
A 		108.2
(2.4)
P 		83
C 60.4
(-1.7)
P 		39
(-3)
A 		40.2
(-2.9)
P 		137
(3)
P 		98
avr 82.9
(0.8) 		53.4
(-1.3) 		30.7
(-3) 		112.7
(3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 55
(-1.5)
A 		89.5
(3)
P 		77.4
(1.4)
P 		61.5
(-0.7)
P 		43.5
(-3)
M 		88.6
(2.9)
P 		46
B 72.9
(1.8)
P 		65.1
(0.8)
P 		34.6
(-3)
A 		82.6
(3)
M 		44.7
(-1.7)
P 		59.3
(0.1)
P 		48
C 83.3
(3)
P 		55.5
(-1)
P 		42.1
(-3)
P 		42.7
(-2.9)
A 		65.4
(0.4)
P 		83
(3)
P 		41
avr 70.4
(1.5) 		70
(1.4) 		51.4
(-3) 		62.3
(-0.4) 		51.2
(-3) 		77
(3) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 25.9
(3)
P 		6.5
(-2.5)
A 		4.8
(-3)
A 		13.6
(-0.5)
A 		21
B 37.4
(3)
P 		22.6
(0)
P 		7.8
(-3)
A 		23.1
(0.1)
P 		30
C 20
(1.4)
P 		11.2
(-3)
A 		14
(-1.6)
P 		23.1
(3)
P 		12
avr 27.8
(3) 		13.4
(-1.6) 		8.9
(-3) 		19.9
(0.5) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 10.1
(-3)
A 		31.8
(3)
P 		16.8
(-1.1)
P 		15.6
(-1.5)
P 		13.1
(-2.2)
M 		21.6
(0.2)
P 		22
B 25.4
(3)
P 		21.7
(1.7)
P 		8.4
(-3)
A 		21.9
(1.8)
M 		18.2
(0.5)
P 		22.1
(1.8)
P 		17
C 30.9
(3)
P 		16.9
(-0.8)
P 		10.7
(-2.4)
P 		8.6
(-3)
A 		21.2
(0.4)
P 		27.8
(2.2)
P 		22
avr 22.1
(2.1) 		23.5
(2.8) 		12
(-3) 		15.4
(-1.3) 		17.5
(-0.2) 		23.8
(3) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186212_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4480
Sequence Type Exemplar sequence
Representative Public ID F54D10.6 NCBI
Target Description F54D10.6 /REP_DB=WormBase Gene ID /WP=CE19456 /TR=Q9TZ86 /GB=AAC68786.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062258
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
F54D10.6 ENSEMBL cdna:known chromosome:CEL140:II:3814659:3815451:-1 gene:F54D10.6 None
NM_062258 NCBI Caenorhabditis elegans F54D10.6 (F54D10.6) mRNA, complete cds. None
SNAP00000030972 ENSEMBL cdna:SNAP chromosome:CEL140:II:3814924:3815840:-1 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000030985 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3814119-3815450(-) 92.69 100.0
Public Domain and Genome References
Gene Title
Ensembl F54D10.6
Entrez Gene 186234 Entrez gene
SwissProt Q9TZ86 EMBL-EBI
WormBase CE35978 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68786 Hypothetical protein F54D10.6 [Caenorhabditis elegans] ref|NP_494659.2| F54D10.6 [Caenorhabditis elegans] 3.0E-51
blast AAC68786 Hypothetical protein F54D10.6 [Caenorhabditis elegans] ref|NP_494659.2| F54D10.6 [Caenorhabditis elegans] 2.0E-41
blast AAF39769 Hypothetical protein C39B5.4 [Caenorhabditis elegans] ref|NP_497515.1| C39B5.4 [Caenorhabditis elegans] 6.0E-20
blast AAF39769 Hypothetical protein C39B5.4 [Caenorhabditis elegans] ref|NP_497515.1| C39B5.4 [Caenorhabditis elegans] 1.0E-19
blast AAC68929 Hypothetical protein T12B5.3 [Caenorhabditis elegans] ref|NP_497304.1| T12B5.3 [Caenorhabditis elegans] 8.0E-18
blast AAC04395 Hypothetical protein C17B7.11 [Caenorhabditis elegans] ref|NP_503929.1| C17B7.11 [Caenorhabditis elegans] 3.0E-16
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 7.8E-11
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 1.2E-7
Sequence
Target Sequence 
>C. ELEGANS:186212_AT
acaactgtgacttgtgctggacaaaaaaaaagtaaattctgtctcagactgacgactcga
aaagtatgccgaggtttgagagcagctgtcgataaatttggagtttttttcgatgcagtc
ctgttttatatcacggaggaaggtcacattgaaatgaatttgatcgaatttgagatcata
tatgccaatgcagacggtggtggtacaactgtgacttacaatgatgaaccatgccgacga
attgaaggagaaaactttgtggaattagcgttcacggatttgaaaataatcatgaaacac
acttcgatattctatatttctaacgatagaaaggataattgcaaaatcattgaatctctc
atggattttttgaaatcagaaaaatgtattcatgtgaaagagtttaatagattaactcaa
atggagcattggaaaaatgcaaaaacatttcacctttctgatgttaaatttgatagtaac
aagttagaaagtttgtttcactttgaagagattatcatcgaacttgacgagttttcag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAACTGTGACTTGTGCTGGACAAA 463 113 316 Antisense
ATTCTGTCTCAGACTGACGACTCGA 125 11 351 Antisense
GCCGAGGTTTGAGAGCAGCTGTCGA 329 283 383 Antisense
GGAGTTTTTTTCGATGCAGTCCTGT 36 519 415 Antisense
GCAGTCCTGTTTTATATCACGGAGG 51 313 430 Antisense
AGACGGTGGTGGTACAACTGTGACT 501 69 507 Antisense
ATGATGAACCATGCCGACGAATTGA 367 41 536 Antisense
GTGGAATTAGCGTTCACGGATTTGA 384 489 574 Antisense
GAAACACACTTCGATATTCTATATT 613 355 609 Antisense
ATGCAAAAACATTTCACCTTTCTGA 105 45 752 Antisense
ATCATCGAACTTGACGAGTTTTCAG 445 23 829 Antisense
Sequence Source Affymetrix Proprietary Database