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[186207_at]

Description Y37D8A.15 /REP_DB=WormBase Gene ID /WP=CE20219 /TR=Q9XWV7 /GB=CAA21533.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 06      6grp_high1_10_10 > 20      4grp_highest_8_8 > 31      4grp_high_8_8 > 12      6grp_highest_nonorm > 72      6grp_total > 40      4grp_total > 12      6grp_highest_sub > 04      6grp_cgc > 72      6grp_cgc_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 981
1 2 3 4 range
A 563.9
(-1.4)
P 		384.9
(-2.5)
P 		300.3
(-3)
P 		1281.3
(3)
P 		981
B 447.4
(-2.2)
P 		402.1
(-2.6)
P 		355.2
(-3)
P 		1015.6
(3)
P 		660
C 531.5
(0.4)
P 		395
(-1.7)
P 		306
(-3)
P 		701.8
(3)
P 		396
avr 514.3
(-1.3) 		394
(-2.4) 		320.5
(-3) 		999.6
(3) 		679

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 206
1 2 3 4 5 6 range
A 486.9
(2.5)
P 		405.9
(-2.6)
P 		494.4
(3)
P 		414.1
(-2.1)
P 		426.9
(-1.3)
P 		399.9
(-3)
P 		95
B 436.1
(-0.9)
P 		487.5
(3)
P 		486.6
(2.9)
P 		465.1
(1.3)
P 		414.8
(-2.6)
P 		409.3
(-3)
P 		78
C 377.2
(-0.9)
P 		466.6
(1.7)
P 		362
(-1.4)
P 		512
(3)
P 		305.9
(-3)
P 		352.5
(-1.6)
P 		206
avr 433.4
(0.8) 		453.3
(2.2) 		447.7
(1.8) 		463.7
(3) 		382.5
(-3) 		387.2
(-2.7) 		81
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 138
1 2 3 4 range
A 205.3
(3)
P 		83.5
(-2.8)
P 		78.5
(-3)
P 		187.6
(2.2)
P 		127
B 142.7
(-0.3)
P 		99.3
(-2.2)
P 		82.3
(-3)
P 		216.4
(3)
P 		134
C 176
(3)
P 		113.6
(-2.4)
P 		106.7
(-3)
P 		118.3
(-2)
P 		69
avr 174.7
(3) 		98.8
(-2.3) 		89.2
(-3) 		174.1
(3) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 79
1 2 3 4 5 6 range
A 89.8
(-3)
P 		144.4
(3)
P 		107.7
(-1)
P 		105
(-1.3)
P 		128.8
(1.3)
P 		97.4
(-2.2)
P 		55
B 151.7
(1)
P 		162.3
(2.3)
P 		118.2
(-3)
P 		123.2
(-2.4)
P 		168.5
(3)
P 		152.6
(1.1)
P 		50
C 139.8
(2.7)
P 		142.4
(3)
P 		92.1
(-3)
P 		103
(-1.7)
P 		99
(-2.2)
P 		118
(0.1)
P 		50
avr 127.1
(-0.1) 		149.7
(3) 		106
(-3) 		110.4
(-2.4) 		132.1
(0.6) 		122.7
(-0.7) 		44
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186207_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7113
Sequence Type Exemplar sequence
Representative Public ID Y37D8A.15 NCBI
Target Description Y37D8A.15 /REP_DB=WormBase Gene ID /WP=CE20219 /TR=Q9XWV7 /GB=CAA21533.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067281(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067281 NCBI Caenorhabditis elegans FMRF-Like Peptide family member (flp-14) (flp-14) mRNA, complete cds. 11/11 None
GENEFINDER00000028807 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:12910039:12911694:1 10/11 None
Y37D8A.15 ENSEMBL cdna:known chromosome:CEL140:III:12910039:12914974:1 gene:Y37D8A.15 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:12910034-12914609(+) 99.85 100.0
Public Domain and Genome References
Gene Title
Gene Symbol flp-14
Ensembl Y37D8A.15
Entrez Gene 176708 Entrez gene
SwissProt Q9XWV7 EMBL-EBI
WormBase CE20219 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7218 neuropeptide signaling pathway inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA21533 Hypothetical protein Y37D8A.15 [Caenorhabditis elegans] ref|NP_499682.1| FMRF-Like Peptide family member (flp-14) [Caenorhabditis elegans] 1.0E-89
blast CAA21533 Hypothetical protein Y37D8A.15 [Caenorhabditis elegans] ref|NP_499682.1| FMRF-Like Peptide family member (flp-14) [Caenorhabditis elegans] 1.0E-53
blast CAE69552 Hypothetical protein CBG15764 [Caenorhabditis briggsae] 7.0E-52
blast AAO18223 FMRFamide-like propeptide [Oesophagostomum dentatum] 3.0E-39
blast CAE69552 Hypothetical protein CBG15764 [Caenorhabditis briggsae] 2.0E-20
blast AAO18223 FMRFamide-like propeptide [Oesophagostomum dentatum] 3.0E-11
Protein Domains
Database ID Description E-Value
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.021
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.4
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.4
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.26
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499682.1 1 15-37
Y37D8A.15 1 15-37
GENEFINDER00000028807 1 15-37
Sequence
Target Sequence 
>C. ELEGANS:186207_AT
attacttccggggcagattctcagattgctaccgtactcttgaattttgtttcatttttt
tttcttctcctcgagctctaccaagccacgcccacaacatttcactttcaacaccgagct
ccgcccattttgaagtctcctctggctccaactgagagctctcttcatatgatgatctgc
ctgcccaccgcgcttctgctctccgcgttcgtcgtggccgccagtggccaggaggcaccg
gctggagccggagcttctggagccgcacaggctccacacaatccgaaggattgtcaagcg
attctggccaacaacggcgatcaacaagaggcgcttttgtgccaattaagcgagagcagc
atgcttcttgctcagctcggagctctcgtttcggagggagttgaacgtctcgttcagact
cacggattggctctcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTACTTCCGGGGCAGATTCTCAGA 152 13 73 Antisense
GATTCTCAGATTGCTACCGTACTCT 536 431 88 Antisense
AACATTTCACTTTCAACACCGAGCT 159 139 168 Antisense
TCTCCTCTGGCTCCAACTGAGAGCT 226 611 208 Antisense
CTTCATATGATGATCTGCCTGCCCA 355 221 235 Antisense
GGAGGCACCGGCTGGAGCCGGAGCT 235 497 303 Antisense
CAGGCTCCACACAATCCGAAGGATT 443 209 340 Antisense
GTCAAGCGATTCTGGCCAACAACGG 671 455 365 Antisense
AAGCGAGAGCAGCATGCTTCTTGCT 54 149 420 Antisense
GGAGCTCTCGTTTCGGAGGGAGTTG 609 519 451 Antisense
TTCAGACTCACGGATTGGCTCTCGA 9 687 485 Antisense
Sequence Source Affymetrix Proprietary Database