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[186197_at] 7TM chemoreceptor, sri family /// --- /// 7TM chemoreceptor, sri family /// ---

Description Y27F2A.2 /REP_DB=WormBase Gene ID /WP=CE26054 /TR=Q9N547 /GB=AAF60479.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 05      4grp_total > 28      6grp_cgc > 42      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 2.5
(-3)
A 		28.9
(3)
A 		6.8
(-2)
A 		12.6
(-0.7)
A 		26
B 29.2
(3)
A 		4.5
(-2.8)
A 		3.5
(-3)
A 		4.5
(-2.8)
A 		26
C 19.6
(-2)
A 		32.2
(-0.5)
A 		10.6
(-3)
A 		62.8
(3)
P 		52
avr 17.1
(0.1) 		21.9
(1.6) 		7
(-3) 		26.6
(3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 34.7
(1.7)
A 		22.4
(-0.2)
A 		14
(-1.4)
A 		5.9
(-2.6)
A 		43.5
(3)
P 		3.5
(-3)
A 		40
B 12.2
(0.1)
A 		5.4
(-2.1)
A 		20.9
(3)
A 		2.6
(-3)
A 		9.6
(-0.7)
A 		3.7
(-2.6)
A 		18
C 9.9
(0)
A 		18.7
(2.9)
A 		2.6
(-2.4)
A 		19.1
(3)
A 		0.7
(-3)
A 		5.7
(-1.4)
A 		18
avr 18.9
(3) 		15.5
(1.6) 		12.5
(0.4) 		9.2
(-1) 		17.9
(2.6) 		4.3
(-3) 		15
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 0.9
(-3)
A 		6.3
(3)
A 		1.8
(-2)
A 		1.8
(-2)
A 		5
B 9.3
(3)
A 		1.1
(-2.8)
A 		0.8
(-3)
A 		1
(-2.9)
A 		9
C 6.5
(-0.6)
A 		9.3
(1.9)
A 		3.7
(-3)
A 		10.6
(3)
P 		7
avr 5.6
(3) 		5.6
(3) 		2.1
(-3) 		4.5
(1.1) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 6.4
(-0.3)
A 		8
(0.5)
A 		3
(-2)
A 		1.5
(-2.7)
A 		13.1
(3)
P 		0.9
(-3)
A 		12
B 4.2
(1.8)
A 		1.8
(-1.5)
A 		5.1
(3)
A 		0.7
(-3)
A 		3.9
(1.4)
A 		1.4
(-2)
A 		4
C 3.7
(0.8)
A 		5.7
(3)
A 		0.7
(-2.5)
A 		3.8
(0.9)
A 		0.2
(-3)
A 		1.9
(-1.1)
A 		6
avr 4.8
(1.7) 		5.2
(2.3) 		2.9
(-0.9) 		2
(-2.2) 		5.7
(3) 		1.4
(-3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186197_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4883
Sequence Type Exemplar sequence
Representative Public ID Y27F2A.2 NCBI
Target Description Y27F2A.2 /REP_DB=WormBase Gene ID /WP=CE26054 /TR=Q9N547 /GB=AAF60479.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027252, NM_001026939, NM_062066, NM_061927
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000023628 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2668590:2673587:-1 None
NM_001026939 NCBI Caenorhabditis elegans F22E5.20 (F22E5.20) mRNA, complete cds. None
GENEFINDER00000006777 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3183796:3188451:1 None
F22E5.20 ENSEMBL cdna:known chromosome:CEL140:II:2672693:2673587:-1 gene:F22E5.20 None
SNAP00000023618 ENSEMBL cdna:SNAP chromosome:CEL140:II:2672693:2673587:-1 None
SNAP00000006769 ENSEMBL cdna:SNAP chromosome:CEL140:II:3184118:3184510:1 None
NM_001027252 NCBI Caenorhabditis elegans Y27F2A.8 (Y27F2A.8) mRNA, complete cds. None
Y27F2A.8 ENSEMBL cdna:known chromosome:CEL140:II:3183796:3184572:1 gene:Y27F2A.8 None
GENEFINDER00000006777 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3183796:3188451:1 None
NM_061927 NCBI Caenorhabditis elegans Serpentine Receptor, class I family member (sri-57) (sri-57) mRNA, complete cds. None
F22E5.16 ENSEMBL cdna:known chromosome:CEL140:II:2670553:2672120:-1 gene:F22E5.16 None
NM_062066 NCBI Caenorhabditis elegans Serpentine Receptor, class I family member (sri-78) (sri-78) mRNA, complete cds. A 
SNAP00000006770 ENSEMBL cdna:SNAP chromosome:CEL140:II:3186349:3188451:1 None
GENEFINDER00000023628 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2668590:2673587:-1 None
Y27F2A.2 ENSEMBL cdna:known chromosome:CEL140:II:3185153:3186785:1 gene:Y27F2A.2 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3183794-3186959(+) 100.0 100.0
chrII:2670444-2673586(-) 91.02 91.02
Public Domain and Genome References
Gene Title 7TM chemoreceptor, sri family

7TM chemoreceptor, sri family

Gene Symbol sri-57
sri-78
Ensembl F22E5.16
F22E5.20
Y27F2A.8
Y27F2A.2
Entrez Gene 184844 Entrez gene
189558 Entrez gene
3565506 Entrez gene
3565664 Entrez gene
SwissProt O16718 EMBL-EBI
Q7KQ96 EMBL-EBI
Q9N547 EMBL-EBI
Q86MI6 EMBL-EBI
WormBase CE33622 Wormbase
CE33623 Wormbase
CE33727 Wormbase
CE33729 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_494328 Serpentine Receptor, class I family member (sri-57) [Caenorhabditis elegans] gb|AAO91734.1| Serpentine receptor, class i protein 57 [Caenorhabditis elegans] 0.0
blast NP_494467 Serpentine Receptor, class I family member (sri-78) [Caenorhabditis elegans] gb|AAO91686.1| Serpentine receptor, class i protein 78 [Caenorhabditis elegans] 0.0
blast T32076 hypothetical protein F22E5.15 - Caenorhabditis elegans 1.0E-148
blast CAE72393 Hypothetical protein CBG19550 [Caenorhabditis briggsae] 1.0E-129
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 2.7E-20
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 6.5E-5
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 3.1E-29
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 3.0E-43
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 4.3E-5
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.2E-67
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 6.0E-67
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.8E-43
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 3.8E-42
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494328.2 7 10-32,45-67,87-109,122-144,194-216,237-259,269-291
NP_494467.2 7 10-32,45-67,87-109,122-144,194-216,237-259,269-291
F22E5.16 7 10-32,45-67,87-109,122-144,194-216,237-259,269-291
Y27F2A.2 7 10-32,45-67,87-109,122-144,194-216,237-259,269-291
SNAP00000006770 5 25-47,54-76,86-103,116-138,181-203
GENEFINDER00000006777 11 153-172,185-207,254-276,297-316,331-353,380-397,401-420,427-449,464-486,507-529,576-598
GENEFINDER00000023628 9 235-257,270-292,342-364,385-407,489-511,531-553,599-621,648-670,680-702
Sequence
Target Sequence 
>C. ELEGANS:186197_AT
tactagccttttcgttcttcatggcagttttatataacatttttcgaatgttaagtttca
tgaaagttcaaatgtctgacaccacttataagagacatcgagccgccgtatggagcttaa
ttgcacaattcgccacgtcgataatttgctttttaccaccaatttctctggttttcgtag
tgtttttgaagctacctaatcctcaggtaatcgtagagcttctccttgtagtcgcctgcc
tccactcgcccgccaacgtgacagtcctgatgttcacgttcccgccataccggaagtttg
tttgtgtgactattttcaggcaaaaaccaacaggcttggagccgatatctgcaaagtctt
cagtgatgaatcaaaatttttataaattgcaaaaaattccagatggcctgattatcgctg
aaagcttaaaaacgttag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACTAGCCTTTTCGTTCTTCATGGC 289 645 890 Antisense
AGCCTTTTCGTTCTTCATGGCAGTT 147 83 894 Antisense
GCCGCCGTATGGAGCTTAATTGCAC 29 283 991 Antisense
TGGAGCTTAATTGCACAATTCGCCA 443 549 1000 Antisense
CAGGTAATCGTAGAGCTTCTCCTTG 166 207 1093 Antisense
ATCGTAGAGCTTCTCCTTGTAGTCG 627 33 1099 Antisense
GCAAAAACCAACAGGCTTGGAGCCG 286 317 1209 Antisense
AGGCTTGGAGCCGATATCTGCAAAG 306 53 1221 Antisense
ATTCCAGATGGCCTGATTATCGCTG 675 7 1285 Antisense
GATGGCCTGATTATCGCTGAAAGCT 12 407 1291 Antisense
ATCGCTGAAAGCTTAAAAACGTTAG 515 35 1303 Antisense
Sequence Source Affymetrix Proprietary Database