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[186181_at]

Description H14E04.3 /REP_DB=WormBase Gene ID /WP=CE20968 /TR=Q9TYP4 /GB=AAD12810.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 16      4grp_highest_8_8 > 55      4grp_high_8_8 > 10      6grp_highest_nonorm > 70      6grp_total > 25      4grp_total > 21      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 278
1 2 3 4 range
A 323.8
(-0.2)
P 		230.9
(-3)
P 		301.9
(-0.9)
P 		432.9
(3)
P 		202
B 357.1
(-0.2)
P 		322.9
(-1.2)
P 		262
(-3)
P 		467.4
(3)
P 		205
C 499.4
(3)
P 		388.5
(0.6)
P 		221
(-3)
P 		348.9
(-0.2)
P 		278
avr 393.4
(2.1) 		314.1
(-1) 		261.6
(-3) 		416.4
(3) 		155

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 355
1 2 3 4 5 6 range
A 460.6
(3)
P 		383.4
(1.2)
P 		313.9
(-0.4)
P 		199.9
(-3)
P 		220.4
(-2.5)
P 		298.8
(-0.7)
P 		261
B 506.6
(2.7)
P 		519
(3)
P 		312.3
(-1.3)
P 		377.8
(0.1)
P 		228.1
(-3)
P 		248.6
(-2.6)
P 		291
C 524.1
(3)
P 		412.5
(1.1)
P 		279.6
(-1.1)
P 		329.8
(-0.3)
P 		169.5
(-3)
P 		267.7
(-1.3)
P 		355
avr 497.1
(3) 		438.3
(1.8) 		301.9
(-1) 		302.5
(-1) 		206
(-3) 		271.7
(-1.6) 		291
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 115
1 2 3 4 range
A 117.9
(3)
P 		50.1
(-3)
P 		78.9
(-0.5)
P 		63.4
(-1.8)
P 		68
B 113.9
(3)
P 		79.7
(-0.9)
P 		60.7
(-3)
P 		99.6
(1.4)
P 		53
C 165.3
(3)
P 		111.7
(-0)
P 		77
(-2)
P 		58.8
(-3)
P 		107
avr 132.4
(3) 		80.5
(-2.2) 		72.2
(-3) 		73.9
(-2.8) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 144
1 2 3 4 5 6 range
A 84.9
(-0.6)
P 		136.4
(3)
P 		68.4
(-1.8)
P 		50.7
(-3)
P 		66.5
(-1.9)
P 		72.8
(-1.5)
P 		86
B 176.3
(3)
P 		172.7
(2.8)
P 		75.8
(-3)
P 		100.1
(-1.5)
P 		92.7
(-2)
P 		92.7
(-2)
P 		101
C 194.3
(3)
P 		125.9
(0.1)
P 		71.2
(-2.3)
P 		66.3
(-2.5)
P 		54.9
(-3)
P 		89.6
(-1.5)
P 		139
avr 151.8
(3) 		145
(2.5) 		71.8
(-3) 		72.4
(-2.9) 		71.4
(-3) 		85
(-2) 		80
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186181_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7995
Sequence Type Exemplar sequence
Representative Public ID H14E04.3 NCBI
Target Description H14E04.3 /REP_DB=WormBase Gene ID /WP=CE20968 /TR=Q9TYP4 /GB=AAD12810.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065145(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065145 NCBI Caenorhabditis elegans H14E04.3 (H14E04.3) mRNA, complete cds. 11/11 None
GENEFINDER00000039218 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2384935:2386567:-1 11/11 None
H14E04.3 ENSEMBL cdna:known chromosome:CEL140:III:2384935:2386567:-1 gene:H14E04.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2384934-2386567(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H14E04.3
Entrez Gene 186743 Entrez gene
SwissProt Q9TYP4 EMBL-EBI
WormBase CE20968 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAD12810 Hypothetical protein H14E04.3 [Caenorhabditis elegans] ref|NP_497546.1| H14E04.3 [Caenorhabditis elegans] 1.0E-102
blast CAE69460 Hypothetical protein CBG15655 [Caenorhabditis briggsae] 7.0E-84
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497546.1 4 17-39,49-68,75-97,123-145
H14E04.3 4 17-39,49-68,75-97,123-145
GENEFINDER00000039218 4 17-39,49-68,75-97,123-145
Sequence
Target Sequence 
>C. ELEGANS:186181_AT
atttcatttgtctacatgctccgaatagcttttgagctcgtcgccaccgaaactgtggag
gcggatattcagtgggaagtaaccgagtgtctttttgtggttttcggtgttcttatctca
attgcacttcacaatgcctatgatacgcgaatttccctcatctcaatttcgtggactatt
tggatgtttattaacacaattttcgcgcttttcgtcgtgaattccgatattattcagctt
gtcatcaagcgaattcagtcgcaaataattttccgcccgctggacattgtgaaattcgtc
ctcgacatcattcacttcttcaatatcccgatttcgttgtacttcatttgtgtcttcttg
gtctcctcgaaaactcacaagcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTTCATTTGTCTACATGCTCCGAA 85 17 85 Antisense
TTTGTGGTTTTCGGTGTTCTTATCT 71 667 178 Antisense
GTTCTTATCTCAATTGCACTTCACA 337 443 193 Antisense
TGCCTATGATACGCGAATTTCCCTC 263 585 219 Antisense
ATTTCCCTCATCTCAATTTCGTGGA 326 17 235 Antisense
TCGCGCTTTTCGTCGTGAATTCCGA 690 599 287 Antisense
CAGTCGCAAATAATTTTCCGCCCGC 640 207 340 Antisense
GAAATTCGTCCTCGACATCATTCAC 590 363 375 Antisense
ATCCCGATTTCGTTGTACTTCATTT 440 37 409 Antisense
ACTTCATTTGTGTCTTCTTGGTCTC 614 103 425 Antisense
TGGTCTCCTCGAAAACTCACAAGCG 163 553 443 Antisense
Sequence Source Affymetrix Proprietary Database