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[186178_s_at]

Description F49C12.10 /REP_DB=WormBase Gene ID /WP=CE03370 /TR=Q20587 /GB=CAA92513.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 12      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 90
1 2 3 4 range
A 53.1
(3)
A 		27
(-3)
A 		35.5
(-1)
A 		48.6
(2)
A 		26
B 29.8
(-3)
A 		33.7
(-2.7)
A 		35.5
(-2.6)
A 		116.8
(3)
M 		87
C 63.4
(1)
A 		78
(3)
A 		33.9
(-3)
A 		71.3
(2.1)
A 		44
avr 48.8
(-1.1) 		46.2
(-1.5) 		35
(-3) 		78.9
(3) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 60
1 2 3 4 5 6 range
A 58.3
(2.9)
A 		7.1
(-3)
A 		43.7
(1.2)
M 		41.9
(1)
A 		59.5
(3)
A 		30.7
(-0.3)
A 		52
B 42
(1)
A 		54.3
(2.3)
M 		44.5
(1.2)
P 		5.5
(-3)
A 		37.6
(0.5)
A 		60.9
(3)
P 		55
C 25
(-3)
A 		25.5
(-2.9)
A 		47
(0.2)
A 		65.8
(3)
P 		49.7
(0.6)
A 		56.6
(1.6)
P 		41
avr 41.8
(0.8) 		29
(-3) 		45.1
(1.7) 		37.7
(-0.4) 		48.9
(2.9) 		49.4
(3) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 19.3
(3)
A 		5.9
(-3)
A 		9.3
(-1.5)
A 		7.1
(-2.5)
A 		13
B 9.5
(-2.5)
A 		8.3
(-3)
A 		8.2
(-3)
A 		24.9
(3)
M 		17
C 21
(2.2)
A 		22.4
(3)
A 		11.8
(-3)
A 		12
(-2.9)
A 		11
avr 16.6
(3) 		12.2
(-0.9) 		9.8
(-3) 		14.7
(1.3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 10.7
(0.2)
A 		2.5
(-3)
A 		9.5
(-0.3)
M 		10.6
(0.2)
A 		17.9
(3)
A 		7.5
(-1.1)
A 		15
B 14.6
(0.7)
A 		18.1
(1.7)
M 		10.8
(-0.4)
P 		1.5
(-3)
A 		15.3
(0.9)
A 		22.7
(3)
P 		21
C 9.3
(-2.2)
A 		7.8
(-3)
A 		12
(-0.8)
A 		13.2
(-0.1)
P 		16.1
(1.4)
A 		19
(3)
P 		11
avr 11.5
(-0.7) 		9.5
(-2.2) 		10.8
(-1.2) 		8.4
(-3) 		16.4
(3) 		16.4
(3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186178_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8845
Sequence Type Exemplar sequence
Representative Public ID F49C12.10 NCBI
Target Description F49C12.10 /REP_DB=WormBase Gene ID /WP=CE03370 /TR=Q20587 /GB=CAA92513.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069232(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069232 NCBI Caenorhabditis elegans F49C12.10 (F49C12.10) mRNA, complete cds. 11/11 None
SNAP00000032698 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9318208:9319194:-1 10/11 None
F49C12.10 ENSEMBL cdna:known chromosome:CEL140:IV:9318208:9319263:-1 gene:F49C12.10 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000032709 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9318213-9319269(-) 99.88 100.0
Public Domain and Genome References
Gene Title
Ensembl F49C12.10
Entrez Gene 177754 Entrez gene
SwissProt Q20587 EMBL-EBI
WormBase CE03370 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92513 Hypothetical protein F49C12.10 [Caenorhabditis elegans] ref|NP_501633.1| F49C12.10 [Caenorhabditis elegans] 1.0E-153
blast CAA92513 Hypothetical protein F49C12.10 [Caenorhabditis elegans] ref|NP_501633.1| F49C12.10 [Caenorhabditis elegans] 1.0E-135
blast CAE74054 Hypothetical protein CBG21706 [Caenorhabditis briggsae] 1.0E-65
blast CAE74054 Hypothetical protein CBG21706 [Caenorhabditis briggsae] 7.0E-54
blast EAM75176 similar to Methylase involved in ubiquinone/menaquinone biosynthesis [Kineococcus radiotolerans SRS30216] ref|ZP_00617126.1| similar to Methylase involved in ubiquinone/menaquinone biosynthesis [Kineococcus radiotolerans SRS30216] 2.0E-15
Sequence
Target Sequence 
>C. ELEGANS:186178_S_AT
gagtcggatcgagctcatatttatctctatgaaaaagcattatcaatgcatccaaattat
ccttattttgaacaatttgaaattcttgaagtgagctgtggtcaagggtactctctcgat
tggatagaaaagtggcatggaccaacaaaatgcatcattggctgtgataaagttgtcact
agaaatatgaataacatagtctacggagatgcactagatttaccgtttgcagataattcg
tttgattttgttttgaatgtggaagcagctcatctttactcggactacaaactatttttc
aaagagggctcacgagttttgcgatcgggtggtgccttctgctacatggacattcgatat
ccccatgaggcaaaacagattcaggagacagccacagcagccggttttgtaaagaaacat
ttcgagttttgcactgatgaagtagttgagggcttaaaatattcagcgagaaaatatgat
aggctactagagaaagctcctttcttcgttaaattcttcaaaaactcgctgcgagcaacg
tactgtgctcctggaacatcaact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGTCGGATCGAGCTCATATTTATC 623 399 217 Antisense
GAGCTGTGGTCAAGGGTACTCTCTC 187 387 309 Antisense
GGAGATGCACTAGATTTACCGTTTG 559 515 421 Antisense
TGGAAGCAGCTCATCTTTACTCGGA 597 545 476 Antisense
GGCTCACGAGTTTTGCGATCGGGTG 703 537 523 Antisense
TGCCTTCTGCTACATGGACATTCGA 135 585 549 Antisense
ACATTCGATATCCCCATGAGGCAAA 278 105 566 Antisense
GATTCAGGAGACAGCCACAGCAGCC 241 431 594 Antisense
GCTCCTTTCTTCGTTAAATTCTTCA 610 297 712 Antisense
AACTCGCTGCGAGCAACGTACTGTG 282 141 739 Antisense
GTACTGTGCTCCTGGAACATCAACT 366 459 756 Antisense
Sequence Source Affymetrix Proprietary Database