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[186170_at]

Description F36F12.3 /REP_DB=WormBase Gene ID /WP=CE17783 /TR=O76396 /GB=AAC19202.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 82      4grp_total > 25      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 5.9
(-2.1)
A 		5.6
(-3)
A 		7.4
(2.1)
A 		7.7
(3)
A 		2
B 4.9
(-2.3)
A 		7.2
(3)
A 		5.7
(-0.5)
A 		4.6
(-3)
A 		3
C 4.9
(-3)
A 		6.5
(-1.2)
A 		5.2
(-2.7)
A 		10.2
(3)
A 		5
avr 5.2
(-3) 		6.4
(0.1) 		6.1
(-0.7) 		7.5
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7
1 2 3 4 5 6 range
A 6.9
(-0.5)
A 		4.9
(-2.3)
A 		10.6
(3)
A 		5.8
(-1.5)
A 		5.5
(-1.8)
A 		4.2
(-3)
A 		6
B 5.2
(-1.4)
A 		6.3
(-0.2)
A 		7.3
(0.9)
A 		3.8
(-3)
A 		9.2
(3)
A 		4.1
(-2.7)
A 		5
C 4.1
(-3)
A 		8.9
(3)
A 		5.1
(-1.8)
A 		6.2
(-0.4)
A 		8.7
(2.8)
A 		5.3
(-1.5)
A 		5
avr 5.4
(-1.4) 		6.7
(1) 		7.7
(2.8) 		5.3
(-1.5) 		7.8
(3) 		4.5
(-3) 		3
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1
1 2 3 4 range
A 2.1
(3)
A 		1.2
(-2.4)
A 		1.9
(1.8)
A 		1.1
(-3)
A 		1
B 1.6
(1.5)
A 		1.8
(3)
A 		1.3
(-0.8)
A 		1
(-3)
A 		1
C 1.6
(-3)
A 		1.9
(3)
A 		1.8
(1)
A 		1.7
(-1)
A 		0
avr 1.8
(3) 		1.6
(0.6) 		1.7
(1.8) 		1.3
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3
1 2 3 4 5 6 range
A 1.3
(-1.6)
A 		1.7
(0.2)
A 		2.3
(3)
A 		1.5
(-0.7)
A 		1.7
(0.2)
A 		1
(-3)
A 		1
B 1.8
(-1.2)
A 		2.1
(-0.6)
A 		1.8
(-1.2)
A 		1
(-3)
A 		3.7
(3)
A 		1.5
(-1.9)
A 		3
C 1.5
(-1.9)
A 		2.7
(2.6)
A 		1.3
(-2.6)
A 		1.2
(-3)
A 		2.8
(3)
A 		1.8
(-0.8)
A 		2
avr 1.5
(-1.8) 		2.2
(1) 		1.8
(-0.6) 		1.2
(-3) 		2.7
(3) 		1.4
(-2.2) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186170_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14938
Sequence Type Exemplar sequence
Representative Public ID F36F12.3 NCBI
Target Description F36F12.3 /REP_DB=WormBase Gene ID /WP=CE17783 /TR=O76396 /GB=AAC19202.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071163
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
F36F12.3 ENSEMBL cdna:known chromosome:CEL140:V:2086215:2087144:1 gene:F36F12.3 None
NM_071163 NCBI Caenorhabditis elegans F36F12.3 (F36F12.3) mRNA, complete cds. None
SNAP00000033954 ENSEMBL cdna:SNAP chromosome:CEL140:V:2087124:2089575:1 None
GENEFINDER00000033966 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2086183:2091443:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2086182-2087406(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F36F12.3
Entrez Gene 185362 Entrez gene
SwissProt O76396 EMBL-EBI
WormBase CE35869 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE66795 Hypothetical protein CBG12156 [Caenorhabditis briggsae] 1.0E-135
blast AAC19207 Hypothetical protein F36F12.8 [Caenorhabditis elegans] ref|NP_503566.1| F36F12.8 [Caenorhabditis elegans] 1.0E-117
blast CAE66795 Hypothetical protein CBG12156 [Caenorhabditis briggsae] 1.0E-115
blast CAE73921 Hypothetical protein CBG21533 [Caenorhabditis briggsae] 1.0E-69
blast AAC19203 Hypothetical protein F36F12.4 [Caenorhabditis elegans] ref|NP_503565.1| F36F12.4 [Caenorhabditis elegans] 3.0E-68
blast AAC19202 Hypothetical protein F36F12.3 [Caenorhabditis elegans] ref|NP_503564.2| F36F12.3 [Caenorhabditis elegans] 3.0E-57
blast CAE73921 Hypothetical protein CBG21533 [Caenorhabditis briggsae] 2.0E-55
blast CAE66795 Hypothetical protein CBG12156 [Caenorhabditis briggsae] 4.0E-41
Protein Domains
Database ID Description E-Value
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 1.4E-7
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 1.6E-34
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 5.2E-38
Sequence
Target Sequence 
>C. ELEGANS:186170_AT
gactacatcgcagtcacgacgacgattcaattcagaactggaaagcctgcctactgccac
ttctatgactgtcaccagcaggagatttccaattccacgtggcgctcctcatttttccct
ctgaacattgtgtactgtgtgcggagagccggagccaagtacatatcgatttctttagaa
aagcacagcgttgcagaaaaaccaccgatccccatgattttcagagcaaataaaatttcc
caaaatttttcccaattttcagaaccaattcacgagttgacaatgtgcgtggggccgttc
tacggtgccgatagaaaatggctccagcttgtggaatttgtggagcacatgcggatattt
gaagtatcaatgttcttctttactgtattcgatatggatgcgtattcccgtctggcgttg
gatgaatacgagcggcaggggatcgccgagactacagtaatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTACATCGCAGTCACGACGACGA 709 369 223 Antisense
CAGAACTGGAAAGCCTGCCTACTGC 382 203 255 Antisense
TGCCACTTCTATGACTGTCACCAGC 223 587 277 Antisense
AGGAGATTTCCAATTCCACGTGGCG 433 55 302 Antisense
CTCATTTTTCCCTCTGAACATTGTG 300 229 330 Antisense
TGTGTACTGTGTGCGGAGAGCCGGA 84 553 351 Antisense
GAGCCGGAGCCAAGTACATATCGAT 698 385 368 Antisense
CCGATCCCCATGATTTTCAGAGCAA 657 263 427 Antisense
AGTTGACAATGTGCGTGGGGCCGTT 416 65 497 Antisense
GTATTCCCGTCTGGCGTTGGATGAA 139 453 624 Antisense
GGGGATCGCCGAGACTACAGTAATC 551 495 660 Antisense
Sequence Source Affymetrix Proprietary Database