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[186154_at]

Description T24A6.18 /REP_DB=WormBase Gene ID /WP=CE28489 /TR=O61948 /GB=AAC17806.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 19      4grp_total > 40      6grp_cgc > 99      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 6.1
(-3)
A 		14.1
(1.7)
A 		15
(2.2)
A 		16.4
(3)
A 		10
B 6.7
(-3)
A 		6.7
(-3)
A 		36.4
(3)
A 		6.6
(-3)
A 		30
C 10.1
(-2.7)
A 		9.2
(-3)
A 		27.6
(3)
A 		15.6
(-0.9)
A 		18
avr 7.6
(-3) 		10
(-2.2) 		26.3
(3) 		12.9
(-1.3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 46.5
(3)
A 		4.2
(-3)
A 		22.1
(-0.4)
A 		20.7
(-0.6)
A 		4
(-3)
A 		20.9
(-0.6)
A 		43
B 6.8
(-3)
A 		7.2
(-2.9)
A 		16.9
(-0.2)
A 		12.7
(-1.4)
A 		28.8
(3)
A 		7.8
(-2.7)
A 		22
C 6.3
(-3)
A 		8.6
(-2.6)
A 		9.7
(-2.4)
A 		8.1
(-2.7)
A 		40.3
(3)
A 		8.1
(-2.7)
A 		34
avr 19.9
(1.5) 		6.7
(-3) 		16.2
(0.2) 		13.8
(-0.6) 		24.4
(3) 		12.3
(-1.1) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 2.2
(-3)
A 		3.1
(0.2)
A 		3.9
(3)
A 		2.4
(-2.3)
A 		2
B 2.1
(-2.4)
A 		1.6
(-2.8)
A 		8.4
(3)
A 		1.4
(-3)
A 		7
C 3.3
(-2.4)
A 		2.7
(-2.9)
A 		9.6
(3)
A 		2.6
(-3)
A 		7
avr 2.5
(-2.5) 		2.5
(-2.5) 		7.3
(3) 		2.1
(-3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 8.6
(3)
A 		1.5
(-2.8)
A 		4.8
(-0.1)
A 		5.2
(0.2)
A 		1.2
(-3)
A 		5.1
(0.2)
A 		7
B 2.4
(-3)
A 		2.4
(-3)
A 		4.1
(-1.9)
A 		3.4
(-2.4)
A 		11.7
(3)
A 		2.9
(-2.7)
A 		9
C 2.3
(-2.6)
A 		2.6
(-2.5)
A 		2.5
(-2.5)
A 		1.6
(-3)
A 		13
(3)
A 		2.7
(-2.4)
A 		11
avr 4.4
(-0.9) 		2.2
(-3) 		3.8
(-1.5) 		3.4
(-1.9) 		8.6
(3) 		3.6
(-1.7) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186154_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15600
Sequence Type Exemplar sequence
Representative Public ID T24A6.18 NCBI
Target Description T24A6.18 /REP_DB=WormBase Gene ID /WP=CE28489 /TR=O61948 /GB=AAC17806.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071596
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000027429 ENSEMBL cdna:SNAP chromosome:CEL140:V:3552661:3554993:-1 None
NM_071596 NCBI Caenorhabditis elegans GRound-Like (grd related) family member (grl-29) (grl-29) mRNA, complete cds. None
T24A6.18 ENSEMBL cdna:known chromosome:CEL140:V:3554437:3555062:-1 gene:T24A6.18 None
GENEFINDER00000027440 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3554437:3554993:-1 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3553939-3555062(-) 88.43 100.0
Public Domain and Genome References
Gene Title
Gene Symbol grl-29
Ensembl T24A6.18
Entrez Gene 188835 Entrez gene
SwissProt O61948 EMBL-EBI
WormBase CE28489 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK73895 Ground-like (grd related) protein 29 [Caenorhabditis elegans] ref|NP_503997.1| GRound-Like (grd related) family member (grl-29) [Caenorhabditis elegans] 4.0E-49
blast AAK73895 Ground-like (grd related) protein 29 [Caenorhabditis elegans] ref|NP_503997.1| GRound-Like (grd related) family member (grl-29) [Caenorhabditis elegans] 9.0E-49
blast B89005 protein T24A6.18 [imported] - Caenorhabditis elegans 1.0E-48
blast B89005 protein T24A6.18 [imported] - Caenorhabditis elegans 3.0E-48
blast AAF39799 Ground-like (grd related) protein 27 [Caenorhabditis elegans] ref|NP_504006.1| GRound-Like (grd related) family member (grl-27) [Caenorhabditis elegans] 1.0E-36
blast AAF39799 Ground-like (grd related) protein 27 [Caenorhabditis elegans] ref|NP_504006.1| GRound-Like (grd related) family member (grl-27) [Caenorhabditis elegans] 2.0E-36
blast AAK73895 Ground-like (grd related) protein 29 [Caenorhabditis elegans] ref|NP_503997.1| GRound-Like (grd related) family member (grl-29) [Caenorhabditis elegans] 1.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 2.4E-26
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 3.0E-12
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 2.4E-26
Sequence
Target Sequence 
>C. ELEGANS:186154_AT
tatgggtgcggaataccaccgcctccaccaccacccccgcagtgcggctgtggcaggaag
aaaagatctcttccggagaaaccgacgttctttggaatttctgcgtcgggggatgatgtg
atgtgcaacacgccagagttgaagaaaattattgcaaggaatatgcaagactccgctatg
gattcttcgaaaaccgtaaatgcagcgttggagaataagaagcttcagagattcacagtg
atttgctcggagaacccgttcgttttctccatccgtgccgacacagcctattgtggggta
cagagaaatggccataattgccatgtgtttgctataagcctaaatttcaattcgattata
actaattcattagaaatttcatacttactcaagcgggtccttattaatttcggggtacag
gaaccccatgctagagtgcttctcccagttgggaatactcttcaaaagatcagccagcaa
gacctcggcggcttcaggagtatctgcgtagaatggagcagggctcagcttgtttttagt
gacaagtcggatggttgcgtacccaacgattttgccaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATGGGTGCGGAATACCACCGCCTC 214 653 193 Antisense
CGCAGTGCGGCTGTGGCAGGAAGAA 344 255 230 Antisense
GATCTCTTCCGGAGAAACCGACGTT 198 313 257 Antisense
ACCGACGTTCTTTGGAATTTCTGCG 491 87 273 Antisense
GAATTTCTGCGTCGGGGGATGATGT 154 325 287 Antisense
GCAAGACTCCGCTATGGATTCTTCG 694 319 357 Antisense
GATTTGCTCGGAGAACCCGTTCGTT 245 335 432 Antisense
CGTGCCGACACAGCCTATTGTGGGG 466 245 466 Antisense
ATTGCCATGTGTTTGCTATAAGCCT 298 7 509 Antisense
TTCATACTTACTCAAGCGGGTCCTT 170 687 570 Antisense
TTGCGTACCCAACGATTTTGCCAGA 51 701 747 Antisense
Sequence Source Affymetrix Proprietary Database