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[186148_s_at]

Description F54D10.7 /REP_DB=WormBase Gene ID /WP=CE19457 /TR=Q9TZ84 /GB=AAC68780.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 88      4grp_total > 44      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 23
(0.6)
A 		4.5
(-3)
A 		35.7
(3)
A 		27.5
(1.4)
A 		31
B 2.7
(-3)
A 		53.3
(3)
A 		11.3
(-2)
A 		2.8
(-3)
A 		51
C 47.7
(3)
A 		45.5
(2.6)
A 		15.6
(-2.9)
A 		15.2
(-3)
A 		33
avr 24.5
(-0.1) 		34.4
(3) 		20.9
(-1.2) 		15.2
(-3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 231
1 2 3 4 5 6 range
A 9.4
(-2.7)
A 		7
(-2.8)
A 		3.1
(-3)
A 		7.4
(-2.8)
A 		92
(1.3)
P 		127.6
(3)
P 		125
B 13.5
(-2.7)
A 		21.8
(-2.4)
A 		2.8
(-3)
A 		24.8
(-2.3)
A 		91.7
(-0.2)
P 		192.1
(3)
P 		189
C 3.7
(-3)
A 		11.7
(-2.8)
A 		5.5
(-3)
A 		6.5
(-2.9)
A 		81.1
(-1)
P 		234.2
(3)
P 		231
avr 8.9
(-2.8) 		13.5
(-2.7) 		3.8
(-3) 		12.9
(-2.7) 		88.3
(-0.2) 		184.6
(3) 		181
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 8.4
(2.3)
A 		1
(-3)
A 		9.3
(3)
A 		4
(-0.8)
A 		8
B 0.9
(-2.9)
A 		13.2
(3)
A 		2.6
(-2)
A 		0.6
(-3)
A 		13
C 15.8
(3)
A 		13.1
(1.8)
A 		5.4
(-1.7)
A 		2.6
(-3)
A 		13
avr 8.4
(2.4) 		9.1
(3) 		5.8
(0) 		2.4
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 1.7
(-2.8)
A 		2.5
(-2.6)
A 		0.7
(-3)
A 		1.9
(-2.8)
A 		27.8
(2.3)
P 		31.1
(3)
P 		30
B 4.7
(-2.7)
A 		7.2
(-2.4)
A 		0.7
(-3)
A 		6.6
(-2.5)
A 		37.2
(0.1)
P 		71.6
(3)
P 		71
C 1.4
(-3)
A 		3.6
(-2.8)
A 		1.4
(-3)
A 		1.3
(-3)
A 		26.3
(-1.1)
P 		78.4
(3)
P 		77
avr 2.6
(-2.8) 		4.4
(-2.6) 		0.9
(-3) 		3.3
(-2.8) 		30.4
(-0) 		60.4
(3) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186148_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5374
Sequence Type Exemplar sequence
Representative Public ID F54D10.7 NCBI
Target Description F54D10.7 /REP_DB=WormBase Gene ID /WP=CE19457 /TR=Q9TZ84 /GB=AAC68780.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062260(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062260 NCBI Caenorhabditis elegans F54D10.7 (F54D10.7) mRNA, complete cds. 11/11 None
SNAP00000030974 ENSEMBL cdna:SNAP chromosome:CEL140:II:3820215:3821274:-1 11/11 None
GENEFINDER00000030980 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3819741:3821274:-1 11/11 None
F54D10.7 ENSEMBL cdna:known chromosome:CEL140:II:3820214:3821278:-1 gene:F54D10.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3820213-3821273(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54D10.7
Entrez Gene 173728 Entrez gene
SwissProt Q9TZ84 EMBL-EBI
WormBase CE19457 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68780 Hypothetical protein F54D10.7 [Caenorhabditis elegans] ref|NP_494661.1| F54D10.7 [Caenorhabditis elegans] 1.0E-117
blast AAC68780 Hypothetical protein F54D10.7 [Caenorhabditis elegans] ref|NP_494661.1| F54D10.7 [Caenorhabditis elegans] 1.0E-116
Protein Domains
Database ID Description E-Value
scop a.2.8.Eukaryotic DNA topoisomerase I, dispensable insert domain All alpha proteins; Long alpha-hairpin; Eukaryotic DNA topoisomerase I, dispensable insert domain; Eukaryotic DNA topoisomerase I, dispensable insert domain 4.90000009536743
Pfam IPR000626 EMBL-EBI Ubiquitin 8.0E-12
Pfam IPR000626 EMBL-EBI Ubiquitin 6.6E-11
Sequence
Target Sequence 
>C. ELEGANS:186148_S_AT
gaagatccaagacgctttcaacaaagaaatgctcgacgcaatcagatctatgaagaaggc
gtttgctcgggagaaggaggagttcagcgatgatttggagattttcaaggaggagctgtt
tgagaagcttgatgaggataatgagacctctgaagacgaagatgaggaggaggagaatga
tgttttggatgcgatcaagttgctccaaactggactcgatgctatgaaagaggcacagag
ggaatttttcaagaaagtgactgaggatactcaagccatccggagaaagattggctcgaa
aaacataaagttcaatgttatccttcacggaagccgaatcccgatggaactggactccct
tgacacaatctacacggtcggaatggccctcctgaagcacggtggcctcgcactgcatcg
ccaacgtctcatgctcaatgacaaggagcttcagtataatcaaactcttgaggaagctgg
aattaaggacggttctgtcatcaagcaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGATCCAAGACGCTTTCAACAAA 133 337 255 Antisense
ATGCTCGACGCAATCAGATCTATGA 291 43 283 Antisense
GAAGGCGTTTGCTCGGGAGAAGGAG 26 333 309 Antisense
GTTGCTCCAAACTGGACTCGATGCT 624 439 453 Antisense
GACTGAGGATACTCAAGCCATCCGG 641 375 513 Antisense
TATCCTTCACGGAAGCCGAATCCCG 607 657 573 Antisense
CGAATCCCGATGGAACTGGACTCCC 547 251 589 Antisense
CTCCCTTGACACAATCTACACGGTC 551 233 609 Antisense
AATCTACACGGTCGGAATGGCCCTC 213 165 621 Antisense
GCCAACGTCTCATGCTCAATGACAA 36 279 674 Antisense
AAGGACGGTTCTGTCATCAAGCAAC 710 159 739 Antisense
Sequence Source Affymetrix Proprietary Database