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[186144_at]

Description F21D12.2 /REP_DB=WormBase Gene ID /WP=CE02667 /TR=Q19675 /GB=AAC70883.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 52      4grp_total > 17      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 5.6
(-3)
A 		38.9
(0.4)
A 		32.4
(-0.2)
A 		63.9
(3)
A 		58
B 9.2
(-3)
A 		26.7
(3)
A 		23.5
(1.9)
A 		24.5
(2.2)
A 		18
C 23.1
(-1.6)
A 		10.8
(-3)
A 		19.4
(-2)
A 		62.4
(3)
P 		52
avr 12.6
(-3) 		25.5
(-0.9) 		25.1
(-1) 		50.3
(3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 54.9
(2.9)
A 		11.2
(-3)
A 		45.5
(1.6)
P 		55.6
(3)
A 		36
(0.4)
A 		21.6
(-1.6)
A 		44
B 3
(-3)
A 		18.7
(-1)
A 		49.6
(3)
A 		15.9
(-1.3)
A 		28
(0.2)
A 		24.1
(-0.3)
A 		47
C 5
(-2.9)
A 		42.1
(3)
P 		23.1
(-0)
A 		4.5
(-3)
A 		23.3
(-0)
A 		14.9
(-1.3)
A 		38
avr 21
(-2.8) 		24
(-1.8) 		39.4
(3) 		25.3
(-1.4) 		29.1
(-0.2) 		20.2
(-3) 		19
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 2.1
(-3)
A 		8.5
(2.3)
A 		8.5
(2.3)
A 		9.4
(3)
A 		7
B 2.9
(-3)
A 		6.6
(3)
A 		5.5
(1.2)
A 		5.2
(0.7)
A 		4
C 7.6
(0.6)
A 		3.1
(-3)
A 		6.8
(-0)
A 		10.5
(3)
P 		7
avr 4.2
(-3) 		6.1
(-0.3) 		6.9
(0.9) 		8.4
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 10.1
(0.6)
A 		4
(-3)
A 		9.9
(0.5)
P 		14.1
(3)
A 		10.9
(1.1)
A 		5.3
(-2.2)
A 		10
B 1
(-3)
A 		6.2
(-0.2)
A 		12.1
(3)
A 		4.2
(-1.3)
A 		11.4
(2.6)
A 		9
(1.3)
A 		11
C 1.8
(-2.5)
A 		12.8
(3)
P 		5.9
(-0.5)
A 		0.9
(-3)
A 		7.5
(0.3)
A 		5
(-0.9)
A 		12
avr 4.3
(-3) 		7.7
(0.6) 		9.3
(2.4) 		6.4
(-0.8) 		9.9
(3) 		6.4
(-0.8) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186144_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5362
Sequence Type Exemplar sequence
Representative Public ID F21D12.2 NCBI
Target Description F21D12.2 /REP_DB=WormBase Gene ID /WP=CE02667 /TR=Q19675 /GB=AAC70883.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063130(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063130 NCBI Caenorhabditis elegans F21D12.2 (F21D12.2) mRNA, complete cds. 11/11 None
GENEFINDER00000020735 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7318615:7319369:1 11/11 None
F21D12.2 ENSEMBL cdna:known chromosome:CEL140:II:7318615:7319084:1 gene:F21D12.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000020728 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7318611-7319081(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F21D12.2
Entrez Gene 184778 Entrez gene
SwissProt Q19675 EMBL-EBI
WormBase CE02667 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC70883 Hypothetical protein F21D12.2 [Caenorhabditis elegans] ref|NP_495531.1| F21D12.2 [Caenorhabditis elegans] 6.0E-60
blast AAC70883 Hypothetical protein F21D12.2 [Caenorhabditis elegans] ref|NP_495531.1| F21D12.2 [Caenorhabditis elegans] 1.0E-58
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495531.1 1 20-42
F21D12.2 1 20-42
GENEFINDER00000020735 1 20-42
Sequence
Target Sequence 
>C. ELEGANS:186144_AT
gtggcgaccaccatcaagttgattcgtagaatccggaagtgtctattgtttttcatgcct
tttttcatgcctacttatgcctattacttatcagcattcttctattcgagcaagagacga
aaaaaaaacgacttcagaccagattgtctagaaaacactgttgtcgaacaaggaaatgat
gaatatgaaccagaaaatgatgaggagacaacctatccggatgatgaaggagattacgat
acttcgataggagtagtggcggagaacatcaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGGCGACCACCATCAAGTTGATTC 111 493 28 Antisense
AGAATCCGGAAGTGTCTATTGTTTT 182 59 55 Antisense
GTGTCTATTGTTTTTCATGCCTTTT 168 487 66 Antisense
TTCATGCCTTTTTTCATGCCTACTT 236 689 79 Antisense
ATGCCTACTTATGCCTATTACTTAT 285 39 94 Antisense
GCCTATTACTTATCAGCATTCTTCT 1 283 106 Antisense
TATCAGCATTCTTCTATTCGAGCAA 73 659 116 Antisense
AAAAAACGACTTCAGACCAGATTGT 188 119 150 Antisense
GATTGTCTAGAAAACACTGTTGTCG 103 433 169 Antisense
GAGGAGACAACCTATCCGGATGATG 450 403 229 Antisense
AGGAGTAGTGGCGGAGAACATCAAC 108 57 276 Antisense
Sequence Source Affymetrix Proprietary Database