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[186123_at]

Description K08B12.3 /REP_DB=WormBase Gene ID /WP=CE11914 /TR=O01581 /GB=AAB52259.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 06      6grp_high1_10_10 > 50      4grp_highest_8_8 > 38      4grp_high_8_8 > 03      6grp_highest_nonorm > 72      6grp_total > 40      4grp_total > 05      6grp_highest_sub > 04      6grp_high1_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 344
1 2 3 4 range
A 362.8
(-1.2)
P 		304.4
(-2.8)
P 		295.8
(-3)
P 		516.4
(3)
P 		221
B 379.9
(-0.1)
P 		214.8
(-3)
P 		269.7
(-2)
P 		558.3
(3)
P 		344
C 407.6
(0.9)
P 		234.4
(-3)
P 		251.6
(-2.6)
P 		500.4
(3)
P 		266
avr 383.4
(-0.1) 		251.2
(-3) 		272.4
(-2.5) 		525
(3) 		274

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 292
1 2 3 4 5 6 range
A 393.8
(-0.5)
P 		503.7
(3)
P 		502.2
(2.9)
P 		505
(3)
P 		313.5
(-3)
P 		369.6
(-1.2)
P 		192
B 339.3
(-2.2)
P 		476.5
(3)
P 		383.6
(-0.5)
P 		454.5
(2.2)
P 		335.9
(-2.3)
P 		318.5
(-3)
P 		158
C 410.3
(-0.5)
P 		419.5
(-0.3)
P 		502.1
(1.4)
P 		578.9
(3)
P 		287.2
(-3)
P 		333.7
(-2)
P 		292
avr 381.1
(-0.9) 		466.6
(1.6) 		462.6
(1.5) 		512.8
(3) 		312.2
(-3) 		340.6
(-2.2) 		201
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 132.1
(3)
P 		66.1
(-3)
P 		77.3
(-2)
P 		75.6
(-2.1)
P 		66
B 121.1
(3)
P 		53
(-3)
P 		62.5
(-2.2)
P 		119
(2.8)
P 		68
C 134.9
(3)
P 		67.4
(-3)
P 		87.7
(-1.2)
P 		84.4
(-1.5)
P 		68
avr 129.4
(3) 		62.2
(-3) 		75.8
(-1.8) 		93
(-0.3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 107
1 2 3 4 5 6 range
A 72.6
(-3)
P 		179.1
(3)
P 		109.4
(-0.9)
P 		128.1
(0.1)
P 		94.6
(-1.8)
P 		90
(-2)
P 		107
B 118.1
(-0.7)
P 		158.6
(3)
P 		93.1
(-3)
P 		120.4
(-0.5)
P 		136.5
(1)
P 		118.8
(-0.6)
P 		66
C 152.1
(3)
P 		128
(0.6)
P 		127.8
(0.5)
P 		116.4
(-0.6)
P 		92.9
(-3)
P 		111.7
(-1.1)
P 		59
avr 114.3
(-2.1) 		155.2
(3) 		110.1
(-2.6) 		121.6
(-1.2) 		108
(-2.9) 		106.8
(-3) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186123_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11322
Sequence Type Exemplar sequence
Representative Public ID K08B12.3 NCBI
Target Description K08B12.3 /REP_DB=WormBase Gene ID /WP=CE11914 /TR=O01581 /GB=AAB52259.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072196(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072196 NCBI Caenorhabditis elegans K08B12.3 (K08B12.3) mRNA, complete cds. 11/11 None
GENEFINDER00000019128 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6237777:6240201:1 11/11 None
K08B12.3 ENSEMBL cdna:known chromosome:CEL140:V:6237777:6240201:1 gene:K08B12.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000019123 4/11 Cross Hyb Matching Probes None
SNAP00000019124 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6237776-6240201(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K08B12.3
Entrez Gene 187136 Entrez gene
SwissProt O01581 EMBL-EBI
WormBase CE11914 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CHICKEN:GGA.12875.1.S1_AT similar to RIKEN cDNA C230094B15 gga
CHICKEN:GGA.9149.1.S1_AT similar to RIKEN cDNA C230094B15 gga
CHICKEN:GGAAFFX.1223.1.S1_AT similar to RIKEN cDNA C230094B15 gga
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52259 Hypothetical protein K08B12.3 [Caenorhabditis elegans] ref|NP_504597.1| K08B12.3 [Caenorhabditis elegans] 1.0E-141
blast CAE71850 Hypothetical protein CBG18893 [Caenorhabditis briggsae] 1.0E-117
blast AAB52259 Hypothetical protein K08B12.3 [Caenorhabditis elegans] ref|NP_504597.1| K08B12.3 [Caenorhabditis elegans] 5.0E-84
blast CAE71850 Hypothetical protein CBG18893 [Caenorhabditis briggsae] 1.0E-68
blast NP_001013491 hypothetical protein LOC541346 [Danio rerio] gb|AAH91457.1| Hypothetical protein LOC541346 [Danio rerio] 3.0E-64
blast XP_392391 PREDICTED: hypothetical protein XP_392391 [Apis mellifera] 2.0E-34
Protein Domains
Database ID Description E-Value
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 2.8E-18
Pfam IPR001888 EMBL-EBI Transposase, type 1 4.8E-14
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 2.0E-17
Sequence
Target Sequence 
>C. ELEGANS:186123_AT
ctactgcaccaacgtctgatcaattgcggattcaaggttgaaaaagaagagatttttacg
agtcttactgctgctcgtgatcttatcgttaagaatcagtaccgtccttttttcattgta
gatgaccgagcaatggaagattttgaaggaatatcaactgacgatccaaatgcagttgtt
atcggtctggcgcctgagaagtttaacgatacgacattgactcacgcgtttaggctcatc
aaagagaaaaaagcttcacttattgccatcaacaagggtagatatcaccagacaaatgcg
ggtctctgcctgggccccggtacgtacgttgcgggattggaatactccgctggtgttgag
gcaacaattgtcggaaagccaaacaagctgttctttgaatctgctcttcaatctctgaat
gaaaacgttgatttttcgtcagctgttatgattggtgatgatgtgaacgatgatgcactg
ggtgcaataaaaataggaatgagagcgattcttgtcaaaactggaaagtttagagatgga
gacgaactgaaagtcaaaaacgtggccaattcttttgttgacgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTACTGCACCAACGTCTGATCAATT 88 227 163 Antisense
GATTTTTACGAGTCTTACTGCTGCT 338 429 213 Antisense
TACTGCTGCTCGTGATCTTATCGTT 80 647 228 Antisense
AGAATCAGTACCGTCCTTTTTTCAT 139 73 254 Antisense
AATGCAGTTGTTATCGGTCTGGCGC 38 165 331 Antisense
GACATTGACTCACGCGTTTAGGCTC 450 369 375 Antisense
AAAGCTTCACTTATTGCCATCAACA 32 125 412 Antisense
CAGACAAATGCGGGTCTCTGCCTGG 538 205 451 Antisense
GGAATACTCCGCTGGTGTTGAGGCA 517 531 501 Antisense
AAGCTGTTCTTTGAATCTGCTCTTC 171 151 547 Antisense
CGTGGCCAATTCTTTTGTTGACGCC 704 243 723 Antisense
Sequence Source Affymetrix Proprietary Database