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[186115_s_at]

Description T05H4.14 /REP_DB=WormBase Gene ID /WP=CE13295 /TR=O16519 /GB=AAB66019.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 77      4grp_high_8_8 > 0      6grp_highest_nonorm > 93      6grp_total > 77      4grp_total > 07      6grp_highest_sub > Da      6grp_cgc > 49      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 141
1 2 3 4 range
A 379.3
(3)
P 		357.8
(-2.5)
P 		355.8
(-3)
P 		373.7
(1.6)
M 		24
B 361.2
(0.4)
P 		280.5
(-3)
P 		360.6
(0.4)
P 		421.1
(3)
P 		141
C 397.9
(3)
P 		285.7
(-3)
P 		355.4
(0.7)
P 		339
(-0.1)
P 		112
avr 379.5
(3) 		308
(-3) 		357.3
(1.1) 		377.9
(2.9) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 307
1 2 3 4 5 6 range
A 343.2
(-2.1)
P 		301
(-3)
P 		338.7
(-2.2)
P 		377.5
(-1.4)
P 		392
(-1.1)
P 		589.1
(3)
P 		288
B 378.4
(-2)
P 		350.2
(-2.8)
P 		344.7
(-3)
P 		385.9
(-1.7)
P 		386.3
(-1.7)
P 		539.5
(3)
P 		195
C 350.3
(-2.3)
P 		314.2
(-3)
P 		344.6
(-2.4)
P 		336.5
(-2.5)
P 		397.8
(-1.3)
P 		607.8
(3)
P 		294
avr 357.3
(-2.2) 		321.8
(-3) 		342.7
(-2.5) 		366.6
(-2) 		392
(-1.4) 		578.8
(3) 		257
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 83
1 2 3 4 range
A 138.1
(3)
P 		77.7
(-1.3)
P 		93
(-0.2)
P 		54.7
(-3)
M 		83
B 115.2
(3)
P 		69.3
(-3)
P 		83.6
(-1.1)
P 		89.7
(-0.3)
P 		46
C 131.7
(3)
P 		82.1
(-1)
P 		123.9
(2.4)
P 		57.2
(-3)
P 		75
avr 128.3
(3) 		76.4
(-2.1) 		100.2
(0.2) 		67.2
(-3) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 140
1 2 3 4 5 6 range
A 63.3
(-3)
P 		107.1
(0.3)
P 		73.8
(-2.2)
P 		95.7
(-0.6)
P 		118.3
(1.1)
P 		143.4
(3)
P 		80
B 131.7
(-0.5)
P 		116.6
(-1.3)
P 		83.7
(-3)
P 		102.2
(-2.1)
P 		157
(0.7)
P 		201.2
(3)
P 		118
C 129.8
(-0.3)
P 		95.9
(-1.8)
P 		87.7
(-2.1)
P 		67.7
(-3)
P 		128.7
(-0.3)
P 		203.5
(3)
P 		136
avr 108.3
(-1.4) 		106.5
(-1.5) 		81.7
(-3) 		88.5
(-2.6) 		134.7
(0.1) 		182.7
(3) 		101
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186115_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11508
Sequence Type Exemplar sequence
Representative Public ID T05H4.14 NCBI
Target Description T05H4.14 /REP_DB=WormBase Gene ID /WP=CE13295 /TR=O16519 /GB=AAB66019.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072234(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072234 NCBI Caenorhabditis elegans GAstrulation Defective family member (gad-1) (gad-1) mRNA, complete cds. 11/11 None
SNAP00000040307 ENSEMBL cdna:SNAP chromosome:CEL140:V:6434033:6436015:-1 11/11 None
GENEFINDER00000040317 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6434033:6436081:-1 11/11 None
T05H4.14 ENSEMBL cdna:known chromosome:CEL140:V:6433935:6436090:-1 gene:T05H4.14 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6434032-6436081(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol gad-1
Ensembl T05H4.14
Entrez Gene 179027 Entrez gene
SwissProt O16519 EMBL-EBI
WormBase CE13295 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:13960_AT transducin family protein / WD-40 repeat family protein at
ATH1-121501:265259_AT transducin family protein / WD-40 repeat family protein at
CANINE_2:CFAAFFX.28528.1.S1_AT similar to CG5543-PA cfa
DROSOPHILA_2:1624247_AT dm
DROSGENOME1:154578_AT dm
CHICKEN:GGAAFFX.12761.1.S1_AT similar to hypothetical protein FLJ10233 gga
CHICKEN:GGAAFFX.2320.1.S1_AT similar to hypothetical protein FLJ10233 gga
HG-U133_PLUS_2:219193_AT WD repeat domain 70 hs
HG-U133A:219193_AT WD repeat domain 70 hs
HG-U133A_2:219193_AT WD repeat domain 70 hs
HU35KSUBA:RC_AA179602_AT WD repeat domain 70 hs
U133_X3P:G8922300_3P_AT WD repeat domain 70 hs
HG-U95B:47912_AT WD repeat domain 70 hs
HG-U95B:44350_AT WD repeat domain 70 hs
HG-U95E:68274_AT WD repeat domain 70 hs
HG-U95D:89736_AT WD repeat domain 70 hs
HG-U95D:90095_AT WD repeat domain 70 hs
HU35KSUBC:RC_N74440_AT WD repeat domain 70 hs
MOUSE430_2:1455097_AT similar to RGD1309487_predicted protein mm
MOE430B:1455097_AT similar to RGD1309487_predicted protein mm
MG-U74BV2:163303_AT similar to RGD1309487_predicted protein mm
RG-U34B:RC_AI012824_AT similar to hypothetical protein FLJ10233 (predicted) rn
RAE230A:1374715_AT similar to hypothetical protein FLJ10233 (predicted) rn
RAT230_2:1374715_AT similar to hypothetical protein FLJ10233 (predicted) rn
RAE230B:1393859_AT Similar to hypothetical protein FLJ10233 (predicted) rn
RAT230_2:1393859_AT Similar to hypothetical protein FLJ10233 (predicted) rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5515 protein binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_504635 GAstrulation Defective family member (gad-1) [Caenorhabditis elegans] gb|AAB66019.1| Gastrulation defective protein 1 [Caenorhabditis elegans] 0.0
blast CAE71878 Hypothetical protein CBG18933 [Caenorhabditis briggsae] 0.0
blast AAH73073 MGC82743 protein [Xenopus laevis] 3.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.3E-4
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0016
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 6.0E-10
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 7.2E-5
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.0E-7
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.33
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.3E-4
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0016
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 6.0E-10
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 7.2E-5
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.0E-7
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.33
Sequence
Target Sequence 
>C. ELEGANS:186115_S_AT
gattaccctggaatttcgtgccaccgaattcagtggcacccgcgattgaatcaaattatc
gctggactctcggatggcaccattcacgtgtactacgatcaaacgatgtcacagcgagga
gttatgtcatgtgtgacgaagccactcaaaagaaatcgcgcatctgaagttgttcgagaa
gatatggttctttctcctctttccctcgaaatgttccaaccgcgcggagaggaaggagaa
gaaaaggaagtcactggatggagaatcaagaagtatctccgaatgcaggataacaagctt
cgtcccgagttccggaagcctgcagatatgcctatcaacggaaagagcgcaaatggacgt
gtggctgcaagtggtggaagtcttcattcgtatttggcaaagcaaatcggaactgctcgt
aatgccgagttcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTACCCTGGAATTTCGTGCCACC 423 429 1255 Antisense
AGTGGCACCCGCGATTGAATCAAAT 334 59 1286 Antisense
AAATTATCGCTGGACTCTCGGATGG 658 115 1307 Antisense
GGCACCATTCACGTGTACTACGATC 12 535 1330 Antisense
GAAATCGCGCATCTGAAGTTGTTCG 87 363 1406 Antisense
AAATGTTCCAACCGCGCGGAGAGGA 518 117 1463 Antisense
GCAGGATAACAAGCTTCGTCCCGAG 618 309 1539 Antisense
GTTCCGGAAGCCTGCAGATATGCCT 285 441 1563 Antisense
TGGACGTGTGGCTGCAAGTGGTGGA 550 547 1608 Antisense
GGAAGTCTTCATTCGTATTTGGCAA 580 531 1630 Antisense
GAACTGCTCGTAATGCCGAGTTCCT 541 347 1664 Antisense
Sequence Source Affymetrix Proprietary Database