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[186073_at]

Description Y27F2A.5 /REP_DB=WormBase Gene ID /WP=CE26057 /TR=Q9N552 /GB=AAF60476.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 14      4grp_total > 36      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 54.9
(0.1)
A 		93
(3)
A 		32
(-1.7)
A 		15.3
(-3)
A 		78
B 51.9
(0)
A 		64.2
(1.8)
A 		30.9
(-3)
A 		72.8
(3)
A 		42
C 58.7
(-0)
A 		100.8
(3)
A 		17.6
(-3)
A 		47.2
(-0.9)
A 		83
avr 55.2
(-0.1) 		86
(3) 		26.8
(-3) 		45.1
(-1.1) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 102.2
(3)
P 		57
(0.3)
A 		20.1
(-2)
A 		83.3
(1.9)
A 		37.1
(-1)
M 		3.5
(-3)
A 		99
B 97.6
(3)
P 		58.9
(-0.5)
M 		81.2
(1.5)
A 		46.7
(-1.6)
A 		44.7
(-1.8)
A 		31
(-3)
A 		67
C 86.9
(1.9)
P 		79.4
(1)
M 		96.2
(3)
A 		70.8
(0)
M 		45.1
(-3)
A 		54.3
(-1.9)
M 		51
avr 95.6
(3) 		65.1
(0.2) 		65.8
(0.3) 		66.9
(0.4) 		42.3
(-1.8) 		29.6
(-3) 		66
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 20
(2.9)
A 		20.2
(3)
A 		8.4
(-0.9)
A 		2.2
(-3)
A 		18
B 16.6
(3)
A 		15.9
(2.6)
A 		7.2
(-3)
A 		15.5
(2.3)
A 		9
C 19.4
(0.5)
A 		29
(3)
A 		6.1
(-3)
A 		8
(-2.5)
A 		23
avr 18.7
(1.8) 		21.7
(3) 		7.2
(-3) 		8.6
(-2.4) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 18.8
(2.3)
P 		20.3
(2.8)
A 		4.4
(-1.9)
A 		21.1
(3)
A 		11.2
(0.1)
M 		0.8
(-3)
A 		20
B 34
(3)
P 		19.6
(-0.8)
M 		19.7
(-0.8)
A 		12.4
(-2.8)
A 		18.1
(-1.2)
A 		11.5
(-3)
A 		23
C 32.2
(3)
P 		24.2
(0.3)
M 		24.5
(0.4)
A 		14.2
(-3)
M 		14.6
(-2.9)
A 		18.2
(-1.7)
M 		18
avr 28.3
(3) 		21.4
(0.7) 		16.2
(-1) 		15.9
(-1.1) 		14.6
(-1.5) 		10.2
(-3) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186073_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4320
Sequence Type Exemplar sequence
Representative Public ID Y27F2A.5 NCBI
Target Description Y27F2A.5 /REP_DB=WormBase Gene ID /WP=CE26057 /TR=Q9N552 /GB=AAF60476.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062063(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062063 NCBI Caenorhabditis elegans Y27F2A.5 (Y27F2A.5) mRNA, complete cds. 11/11 None
SNAP00000006763 ENSEMBL cdna:SNAP chromosome:CEL140:II:3168762:3170872:-1 11/11 None
GENEFINDER00000006774 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3168546:3172226:-1 11/11 None
Y27F2A.5 ENSEMBL cdna:known chromosome:CEL140:II:3168762:3175957:-1 gene:Y27F2A.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3168760-3172724(-) 41.05 52.34
Public Domain and Genome References
Gene Title
Ensembl Y27F2A.5
Entrez Gene 189559 Entrez gene
SwissProt Q9N552 EMBL-EBI
WormBase CE26057 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494464 Y27F2A.5 [Caenorhabditis elegans] gb|AAF60476.2| Hypothetical protein Y27F2A.5 [Caenorhabditis elegans] 0.0
blast AAB95006 Hypothetical protein F33H12.6 [Caenorhabditis elegans] ref|NP_494302.1| F33H12.6 [Caenorhabditis elegans] 0.0
blast NP_494142 ZK250.10 [Caenorhabditis elegans] 0.0
blast AAK71402 Serpentine receptor, class i protein 32 [Caenorhabditis elegans] ref|NP_494461.1| Serpentine Receptor, class I family member (sri-32) [Caenorhabditis elegans] 2.0E-35
blast NP_494463 Serpentine Receptor, class I family member (sri-34) [Caenorhabditis elegans] gb|AAK72091.1| Serpentine receptor, class i protein 34 [Caenorhabditis elegans] 3.0E-32
Protein Domains
Database ID Description E-Value
Pfam IPR010285 EMBL-EBI Eukaryotic protein of unknown function DUF889 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494464.1 4 795-817,855-877,905-927,939-961
Y27F2A.5 4 795-817,855-877,905-927,939-961
GENEFINDER00000006774 3 36-58,95-117,138-160
Sequence
Target Sequence 
>C. ELEGANS:186073_AT
atttggcgattgatcgacccactgcttactggccccctggacactttggaggtcattggg
agctccctttcaatagcgccagcgatatttcaaaacgcccgatgtttgatcttcaatttg
gaacctggaccttattccctacaagtaatctcacaccaacgcgtccatttcaagaatgct
tccatagacacctgcccggaaattgtgcaacttgtgagaaactgggtggaaaaccataag
cccgctggaacatggtactcgtttgatttcaagattgaggtgttgccgaagtcagtgctg
aatgaagtgaagatcaaccatgagaatcttctattggttgaggagggaaattcttctacc
caaatcatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTTGGCGATTGATCGACCCACTGC 243 19 3727 Antisense
CCCCCTGGACACTTTGGAGGTCATT 290 261 3759 Antisense
GGTCATTGGGAGCTCCCTTTCAATA 442 503 3777 Antisense
AACGCCCGATGTTTGATCTTCAATT 351 145 3820 Antisense
TGGACCTTATTCCCTACAAGTAATC 637 545 3852 Antisense
ACACCAACGCGTCCATTTCAAGAAT 594 109 3879 Antisense
AAGAATGCTTCCATAGACACCTGCC 502 151 3898 Antisense
TAGACACCTGCCCGGAAATTGTGCA 398 639 3911 Antisense
TAAGCCCGCTGGAACATGGTACTCG 187 637 3963 Antisense
GAGGTGTTGCCGAAGTCAGTGCTGA 708 401 4003 Antisense
GGGAAATTCTTCTACCCAAATCATC 251 495 4071 Antisense
Sequence Source Affymetrix Proprietary Database