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[186063_at]

Description Y25C1A.6 /REP_DB=WormBase Gene ID /WP=CE21473 /TR=Q9TYM3 /GB=AAD12837.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 67      4grp_total > 43      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 5.6
(-3)
A 		12.3
(3)
A 		9.2
(0.2)
A 		8.1
(-0.8)
A 		7
B 7.8
(-3)
A 		8.4
(-1.7)
A 		10.6
(3)
A 		9.8
(1.3)
A 		3
C 8
(-0.6)
A 		13.4
(3)
A 		4.3
(-3)
A 		6.3
(-1.7)
A 		9
avr 7.1
(-3) 		11.4
(3) 		8
(-1.7) 		8.1
(-1.6) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 12.5
(-2.1)
A 		6.6
(-2.7)
A 		54.6
(1.9)
A 		3
(-3)
A 		9.3
(-2.4)
A 		66
(3)
A 		63
B 14
(-0.9)
A 		8.9
(-2.4)
A 		26.1
(2.6)
A 		6.9
(-3)
A 		15.1
(-0.6)
A 		27.6
(3)
A 		21
C 31
(-0.1)
A 		12.4
(-2.3)
A 		24.8
(-0.9)
A 		6.8
(-3)
A 		48.7
(2)
A 		57.2
(3)
A 		50
avr 19.2
(-1.2) 		9.3
(-2.5) 		35.2
(1) 		5.6
(-3) 		24.4
(-0.5) 		50.3
(3) 		45
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3
1 2 3 4 range
A 2
(0.2)
A 		2.7
(3)
A 		2.4
(1.8)
A 		1.2
(-3)
A 		2
B 2.5
(3)
A 		2.1
(-3)
A 		2.5
(3)
A 		2.1
(-3)
A 		0
C 2.6
(0.2)
A 		3.9
(3)
A 		1.5
(-2.1)
A 		1.1
(-3)
A 		3
avr 2.4
(0.9) 		2.9
(3) 		2.1
(-0.4) 		1.5
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 2.3
(-2.4)
A 		2.4
(-2.4)
A 		11.9
(1.4)
A 		0.8
(-3)
A 		2.8
(-2.2)
A 		16.1
(3)
A 		15
B 4.9
(-0.8)
A 		3
(-2.2)
A 		6.3
(0.2)
A 		1.8
(-3)
A 		6.1
(0)
A 		10.3
(3)
A 		9
C 11.5
(0.4)
A 		3.8
(-2.2)
A 		6.3
(-1.3)
A 		1.4
(-3)
A 		15.8
(1.9)
A 		19.1
(3)
A 		18
avr 6.2
(-0.9) 		3.1
(-2.2) 		8.2
(-0) 		1.3
(-3) 		8.2
(-0) 		15.2
(3) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186063_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4826
Sequence Type Exemplar sequence
Representative Public ID Y25C1A.6 NCBI
Target Description Y25C1A.6 /REP_DB=WormBase Gene ID /WP=CE21473 /TR=Q9TYM3 /GB=AAD12837.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062036(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062036 NCBI Caenorhabditis elegans Y25C1A.6 (Y25C1A.6) mRNA, complete cds. 11/11 None
SNAP00000041876 ENSEMBL cdna:SNAP chromosome:CEL140:II:3058186:3062030:1 11/11 None
GENEFINDER00000041891 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3058233:3062030:1 11/11 None
Y25C1A.6.1 ENSEMBL cdna:known chromosome:CEL140:II:3058253:3062086:1 gene:Y25C1A.6 11/11 None
Y25C1A.6.2 ENSEMBL cdna:known chromosome:CEL140:II:3061095:3062086:1 gene:Y25C1A.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3061162-3062029(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y25C1A.6
Entrez Gene 173653 Entrez gene
SwissProt Q9TYM3 EMBL-EBI
WormBase CE21473 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494437 Y25C1A.6 [Caenorhabditis elegans] gb|AAD12837.1| Hypothetical protein Y25C1A.6 [Caenorhabditis elegans] 1.0E-108
blast NP_494437 Y25C1A.6 [Caenorhabditis elegans] gb|AAD12837.1| Hypothetical protein Y25C1A.6 [Caenorhabditis elegans] 1.0E-107
blast AAB70947 Hypothetical protein B0454.1 [Caenorhabditis elegans] gb|AAZ77787.1| synthetic multivulva protein LIN-8 [Caenorhabditis elegans] ref|NP_494436.1| B0454.1 [Caenorhabditis elegans] 1.0E-102
blast AAB70947 Hypothetical protein B0454.1 [Caenorhabditis elegans] gb|AAZ77787.1| synthetic multivulva protein LIN-8 [Caenorhabditis elegans] ref|NP_494436.1| B0454.1 [Caenorhabditis elegans] 9.0E-94
blast D88088 protein B0454.1 [imported] - Caenorhabditis elegans 9.0E-63
blast D88088 protein B0454.1 [imported] - Caenorhabditis elegans 1.0E-54
Sequence
Target Sequence 
>C. ELEGANS:186063_AT
acagtctccaggacctcctgaacattcactcggtccaagaggacacccagtacaagtcta
tgggaatctacgacttgaaaacgtgcgagatcccaatgagacctccaactcaccagaatc
cggcccagaaggagcacaacactatggaagacgagatgatggccgtgctcaacagctttt
ccagaagaatcgagattatgagtgctccggagcccaaggctttcaggtactacttcgaga
tatccccaccgccgaagccagaagcccccaagtcccagcttggccttggagacctctccg
aagaaagctacccgttgactccggagcctattcagctggacacctgcctcacttggagcc
ctgtcgagctgcaattcgacccgtttgccgagccggagccaattttctacaatgaagaga
tggacagtgggccgacaccggagcccataccggatattttcgagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAGTCTCCAGGACCTCCTGAACAT 593 107 44 Antisense
TCCTGAACATTCACTCGGTCCAAGA 281 593 59 Antisense
GTGCGAGATCCCAATGAGACCTCCA 397 477 126 Antisense
GAATCCGGCCCAGAAGGAGCACAAC 481 329 159 Antisense
GGAGCCCAAGGCTTTCAGGTACTAC 356 521 252 Antisense
GGTACTACTTCGAGATATCCCCACC 538 293 269 Antisense
CCAAGTCCCAGCTTGGCCTTGGAGA 60 277 311 Antisense
GAGACCTCTCCGAAGAAAGCTACCC 271 395 332 Antisense
GCCCTGTCGAGCTGCAATTCGACCC 208 287 401 Antisense
TTTGCCGAGCCGGAGCCAATTTTCT 698 667 427 Antisense
GAGCCCATACCGGATATTTTCGAGT 15 385 484 Antisense
Sequence Source Affymetrix Proprietary Database