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[186060_at]

Description C44B12.3 /REP_DB=WormBase Gene ID /WP=CE24844 /TR=O44142 /GB=AAB88326.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 28      4grp_total > 42      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 146.7
(2.3)
A 		148.6
(2.7)
A 		150.2
(3)
A 		120
(-3)
A 		30
B 87.9
(-2.7)
A 		169.2
(3)
A 		120.1
(-0.5)
A 		84.3
(-3)
A 		85
C 76.6
(-3)
A 		140.9
(2.5)
A 		77.8
(-2.9)
A 		147.2
(3)
A 		71
avr 103.7
(-3) 		152.9
(3) 		116
(-1.5) 		117.2
(-1.4) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 170
1 2 3 4 5 6 range
A 192.8
(3)
A 		98.8
(-1.6)
A 		105.7
(-1.3)
A 		71
(-3)
A 		91
(-2)
A 		114.6
(-0.9)
A 		122
B 118.4
(2.4)
A 		92.1
(-0.2)
A 		92.4
(-0.1)
A 		78.7
(-1.4)
A 		125.1
(3)
A 		62.3
(-3)
A 		63
C 105.1
(1.7)
A 		128.9
(3)
A 		89.9
(0.8)
A 		23.1
(-3)
A 		94.5
(1)
A 		83.8
(0.4)
A 		106
avr 138.8
(3) 		106.6
(0.6) 		96
(-0.2) 		57.6
(-3) 		103.5
(0.4) 		86.9
(-0.8) 		81
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 36
1 2 3 4 range
A 53.4
(3)
A 		32.3
(-0.5)
A 		39.3
(0.6)
A 		17.6
(-3)
A 		36
B 28
(-0.5)
A 		41.8
(3)
A 		27.8
(-0.5)
A 		18
(-3)
A 		24
C 25.4
(-2.8)
A 		40.5
(3)
A 		27.1
(-2.1)
A 		24.8
(-3)
A 		16
avr 35.6
(2.1) 		38.2
(3) 		31.4
(0.7) 		20.1
(-3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 35.5
(3)
A 		35.1
(2.9)
A 		23
(-1.3)
A 		18
(-3)
A 		27.5
(0.3)
A 		27.9
(0.4)
A 		18
B 41.2
(1.1)
A 		30.7
(-1)
A 		22.4
(-2.7)
A 		20.8
(-3)
A 		50.8
(3)
A 		23.2
(-2.5)
A 		30
C 38.9
(2.9)
A 		39.3
(3)
A 		22.9
(0.2)
A 		4.7
(-3)
A 		30.6
(1.5)
A 		28
(1)
A 		35
avr 38.5
(3) 		35
(2.1) 		22.8
(-0.9) 		14.5
(-3) 		36.3
(2.5) 		26.4
(-0) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186060_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10709
Sequence Type Exemplar sequence
Representative Public ID C44B12.3 NCBI
Target Description C44B12.3 /REP_DB=WormBase Gene ID /WP=CE24844 /TR=O44142 /GB=AAB88326.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067637
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000002996 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1104773:1105086:1 None
GENEFINDER00000003005 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:1100516:1102870:1 None
GENEFINDER00000003009 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:1104773:1105086:1 None
C44B12.3 ENSEMBL cdna:known chromosome:CEL140:IV:1104910:1105273:1 gene:C44B12.3 None
NM_067637 NCBI Caenorhabditis elegans C44B12.3 (C44B12.3) mRNA, complete cds. None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_067636 3/11 Cross Hyb Matching Probes A 
SNAP00000002994 3/11 Cross Hyb Matching Probes None
Y55F3AR.1.2 3/11 Cross Hyb Matching Probes None
Y55F3AR.1.1 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1101873-1105279(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C44B12.3
Entrez Gene 183436 Entrez gene
SwissProt O44142 EMBL-EBI
WormBase CE31332 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60807 Hypothetical protein Y55F3AR.1 [Caenorhabditis elegans] ref|NP_500037.2| Y55F3AR.1 [Caenorhabditis elegans] 2.0E-52
blast NP_500038 C44B12.3 [Caenorhabditis elegans] gb|AAB88326.3| Hypothetical protein C44B12.3 [Caenorhabditis elegans] 6.0E-45
blast NP_500038 C44B12.3 [Caenorhabditis elegans] gb|AAB88326.3| Hypothetical protein C44B12.3 [Caenorhabditis elegans] 9.0E-28
blast CAE63861 Hypothetical protein CBG08423 [Caenorhabditis briggsae] 3.0E-26
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000003005 3 35-57,78-97,149-168
Sequence
Target Sequence 
>C. ELEGANS:186060_AT
gaaatcgtacgacggagtcctggcatttttcacaatgttcgccgtgacgattatggctca
attgccggcgctccgaaatccaaaaatcactcgaaaaatcgtagtttttagctcattttt
tcccgaatttcgtccaaaaacccctgatcaccattttcttcaaagtatgatcaagttcgg
aatcgacaagaagtgggctcaaatgatcagcggaattgaggagaaacacgagaaagacaa
agcactgctcgaaaagaagtataatgatgaattggcagctgttgataagagtattccacc
agagcagtacaagcatatgatggattggtatcgtcatgccgaaaaccctgaaaattttaa
cttggaactttggctcattcgagccttctgtccaacagctgtcattatctactcatcatc
atcgtcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAATCGTACGACGGAGTCCTGGCA 276 361 27 Antisense
ACGGAGTCCTGGCATTTTTCACAAT 687 95 38 Antisense
CACAATGTTCGCCGTGACGATTATG 61 195 57 Antisense
CGATTATGGCTCAATTGCCGGCGCT 173 249 74 Antisense
TGCCGGCGCTCCGAAATCCAAAAAT 656 585 89 Antisense
CCTGATCACCATTTTCTTCAAAGTA 48 267 169 Antisense
GTATTCCACCAGAGCAGTACAAGCA 228 453 317 Antisense
GGTATCGTCATGCCGAAAACCCTGA 83 505 353 Antisense
TTGGCTCATTCGAGCCTTCTGTCCA 323 707 396 Antisense
GTCCAACAGCTGTCATTATCTACTC 13 479 416 Antisense
ATTATCTACTCATCATCATCGTCCT 410 13 430 Antisense
Sequence Source Affymetrix Proprietary Database