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[186057_at]

Description Y95B8A.9 /REP_DB=WormBase Gene ID /WP=CE25638 /TR=Q9N2W2 /GB=AAF60908.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 44      6grp_total > 24      4grp_total > 44      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 154
1 2 3 4 range
A 123.4
(-3)
P 		208.6
(3)
P 		127.2
(-2.7)
A 		205.7
(2.8)
P 		85
B 233.9
(3)
P 		230.4
(2.8)
P 		187.8
(0.5)
P 		123.4
(-3)
A 		111
C 233.1
(1.2)
P 		277.7
(3)
P 		193.3
(-0.4)
P 		130.5
(-3)
P 		147
avr 196.8
(0.1) 		238.9
(3) 		169.4
(-1.9) 		153.2
(-3) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 150
1 2 3 4 5 6 range
A 182.5
(3)
P 		162.9
(2)
P 		164
(2.1)
P 		117.4
(-0.2)
A 		89.7
(-1.6)
A 		62.3
(-3)
A 		120
B 211.8
(3)
P 		178.3
(1.3)
P 		114
(-2)
P 		95.5
(-3)
A 		121.6
(-1.7)
P 		110.9
(-2.2)
P 		116
C 153.8
(1.8)
P 		118.5
(-0.5)
P 		172.2
(3)
M 		80.3
(-3)
A 		115.9
(-0.7)
A 		84.7
(-2.7)
A 		92
avr 182.7
(3) 		153.2
(1.2) 		150.1
(1) 		97.7
(-2.3) 		109.1
(-1.6) 		86
(-3) 		97
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 44.9
(2.8)
P 		45.3
(3)
P 		33.2
(-1.8)
A 		30.1
(-3)
P 		15
B 74.6
(3)
P 		56.9
(0.8)
P 		43.5
(-0.9)
P 		26.3
(-3)
A 		48
C 77.2
(2.7)
P 		79.8
(3)
P 		67.4
(1.7)
P 		22
(-3)
P 		58
avr 65.6
(3) 		60.7
(2.3) 		48
(0.3) 		26.1
(-3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 33.6
(-0.4)
P 		57.9
(3)
P 		35.7
(-0.1)
P 		29.8
(-0.9)
A 		27.1
(-1.3)
A 		15.2
(-3)
A 		43
B 73.7
(3)
P 		59.4
(1.2)
P 		27.7
(-2.7)
P 		25.3
(-3)
A 		49.4
(-0)
P 		41.3
(-1)
P 		48
C 57
(3)
P 		36.2
(-0.1)
P 		43.8
(1.1)
M 		16.1
(-3)
A 		37.5
(0.1)
A 		28.4
(-1.2)
A 		41
avr 54.8
(3) 		51.2
(2.3) 		35.7
(-0.7) 		23.7
(-3) 		38
(-0.2) 		28.3
(-2.1) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186057_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22
Sequence Type Exemplar sequence
Representative Public ID Y95B8A.9 NCBI
Target Description Y95B8A.9 /REP_DB=WormBase Gene ID /WP=CE25638 /TR=Q9N2W2 /GB=AAF60908.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182008, NM_058382
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058382 NCBI Caenorhabditis elegans ZC123.4a (ZC123.4) mRNA, complete cds. A 
NM_182008 NCBI Caenorhabditis elegans ZC123.4b (ZC123.4) mRNA, complete cds. A 
ZC123.4c ENSEMBL cdna:known chromosome:CEL140:I:841756:846765:-1 gene:ZC123.4 None
ZC123.4b ENSEMBL cdna:known chromosome:CEL140:I:841753:847613:-1 gene:ZC123.4 A 
ZC123.4a ENSEMBL cdna:known chromosome:CEL140:I:841838:847613:-1 gene:ZC123.4 A 
SNAP00000034251 ENSEMBL cdna:SNAP chromosome:CEL140:I:845616:846747:-1 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000034285 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:845715-847613(-) 64.22 66.24
Public Domain and Genome References
Ensembl ZC123.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB97606 Hypothetical protein ZC123.4a [Caenorhabditis elegans] ref|NP_490783.2| ZC123.4a [Caenorhabditis elegans] 0.0
blast AAU87802 Hypothetical protein ZC123.4b [Caenorhabditis elegans] ref|NP_871808.2| ZC123.4b [Caenorhabditis elegans] 0.0
blast CAE71594 Hypothetical protein CBG18552 [Caenorhabditis briggsae] 0.0
blast AAU87803 Hypothetical protein ZC123.4c [Caenorhabditis elegans] ref|NP_001021842.1| ZC123.4c [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 9.0E-82
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-85
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 5.0E-71
hanks 3.1.7 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; PCTAIRE1 5.0E-76
hanks 3.1.7 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; PCTAIRE1 2.0E-80
hanks 3.1.7 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; PCTAIRE1 1.0E-84
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.6E-87
Pfam IPR000719 EMBL-EBI Protein kinase 8.6E-72
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-80
Sequence
Target Sequence 
>C. ELEGANS:186057_AT
gagaggcctatgagagcatcgtgctcactgtctatgatacatcatccacagctcaccgct
gttaggagttcgaagtcgtcggagaacttgcatagggtgagccacagtcggtcggtaaat
gaggatatttcttgtaagccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaagcctaaannnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnaagcctaaatatttcgattacgctccacatattccagatcaccac
aatgtcgccgcaacgaattcaggacgtggacggctcgccgagcacccggaaggtcaccgt
cctcgtcaattttcactttgcgtcggagtcggaataaggagaagaaatacaagaataacg
gcggaggctccaccaaatct

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAGGCCTATGAGAGCATCGTGCT 157 395 118 Antisense
GCATCGTGCTCACTGTCTATGATAC 345 309 133 Antisense
TATGATACATCATCCACAGCTCACC 698 655 150 Antisense
CAGCTCACCGCTGTTAGGAGTTCGA 307 203 166 Antisense
GTGAGCCACAGTCGGTCGGTAAATG 419 481 214 Antisense
TATTTCGATTACGCTCCACATATTC 380 661 502 Antisense
ATATTCCAGATCACCACAATGTCGC 304 19 521 Antisense
ACAATGTCGCCGCAACGAATTCAGG 113 113 536 Antisense
CGAATTCAGGACGTGGACGGCTCGC 6 253 551 Antisense
TCCTCGTCAATTTTCACTTTGCGTC 707 591 597 Antisense
TAACGGCGGAGGCTCCACCAAATCT 363 585 653 Antisense
Sequence Source Affymetrix Proprietary Database