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[186056_s_at]

Description Y71H2B.2 /REP_DB=WormBase Gene ID /WP=CE24629 /TR=Q9N4E8 /GB=AAF36030.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 86      6grp_high1_10_10 > 67      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 92      6grp_total > 68      4grp_total > 21      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 274
1 2 3 4 range
A 320.1
(2.6)
P 		218.8
(-0.5)
P 		137.2
(-3)
P 		333.4
(3)
P 		196
B 259.5
(-0.7)
P 		245.6
(-1)
P 		164.1
(-3)
P 		410.8
(3)
P 		247
C 322.4
(0.3)
P 		260.9
(-2.3)
P 		244
(-3)
P 		386.1
(3)
P 		142
avr 300.7
(0.7) 		241.8
(-1.2) 		181.8
(-3) 		376.8
(3) 		195

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 367
1 2 3 4 5 6 range
A 218.8
(-2.8)
P 		229.5
(-2.5)
P 		252.3
(-2)
P 		210.3
(-3)
P 		224.7
(-2.7)
P 		459
(3)
P 		249
B 231.6
(-1.1)
P 		151.1
(-3)
P 		197.3
(-1.9)
P 		167.3
(-2.6)
P 		283.5
(0.2)
P 		401.7
(3)
P 		251
C 302.2
(-0.9)
P 		191.4
(-2.9)
P 		185.4
(-3)
P 		223.6
(-2.3)
P 		236.8
(-2.1)
P 		518.5
(3)
P 		333
avr 250.9
(-1.7) 		190.7
(-3) 		211.7
(-2.5) 		200.4
(-2.8) 		248.3
(-1.7) 		459.7
(3) 		269
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 81
1 2 3 4 range
A 116.6
(3)
P 		47.5
(-2.1)
P 		35.9
(-3)
P 		48.8
(-2)
P 		81
B 82.7
(2.4)
P 		60.7
(-0.2)
P 		38
(-3)
P 		87.5
(3)
P 		50
C 106.7
(3)
P 		75
(-1.6)
P 		85
(-0.1)
P 		65.1
(-3)
P 		42
avr 102
(3) 		61.1
(-2) 		53
(-3) 		67.1
(-1.3) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 40.3
(-3)
P 		81.6
(0.5)
P 		54.9
(-1.8)
P 		53.3
(-1.9)
P 		67.8
(-0.7)
P 		111.7
(3)
P 		71
B 80.6
(-0.9)
P 		50.3
(-2.7)
P 		47.9
(-2.8)
P 		44.3
(-3)
P 		115.2
(1)
P 		149.8
(3)
P 		106
C 112
(0.1)
P 		58.4
(-2.4)
P 		47.2
(-2.9)
P 		45
(-3)
P 		76.6
(-1.5)
P 		173.6
(3)
P 		129
avr 77.6
(-1.1) 		63.4
(-2) 		50
(-2.8) 		47.5
(-3) 		86.5
(-0.6) 		145
(3) 		98
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186056_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7146
Sequence Type Exemplar sequence
Representative Public ID Y71H2B.2 NCBI
Target Description Y71H2B.2 /REP_DB=WormBase Gene ID /WP=CE24629 /TR=Q9N4E8 /GB=AAF36030.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065191(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065191 NCBI Caenorhabditis elegans Y71H2B.2 (Y71H2B.2) mRNA, complete cds. 11/11 None
SNAP00000014011 ENSEMBL cdna:SNAP chromosome:CEL140:III:2622377:2627464:1 11/11 None
GENEFINDER00000014041 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2623160:2627464:1 11/11 None
Y71H2B.2 ENSEMBL cdna:known chromosome:CEL140:III:2623159:2627725:1 gene:Y71H2B.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2623159-2627464(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71H2B.2
Entrez Gene 175382 Entrez gene
SwissProt Q9N4E8 EMBL-EBI
WormBase CE24629 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF36030 Hypothetical protein Y71H2B.2 [Caenorhabditis elegans] ref|NP_497592.1| Y71H2B.2 [Caenorhabditis elegans] 1.0E-178
blast AAF36030 Hypothetical protein Y71H2B.2 [Caenorhabditis elegans] ref|NP_497592.1| Y71H2B.2 [Caenorhabditis elegans] 1.0E-174
blast CAE64992 Hypothetical protein CBG09828 [Caenorhabditis briggsae] 1.0E-121
blast CAE64992 Hypothetical protein CBG09828 [Caenorhabditis briggsae] 1.0E-117
blast EAA12336 ENSANGP00000011694 [Anopheles gambiae str. PEST] ref|XP_317409.2| ENSANGP00000011694 [Anopheles gambiae str. PEST] 2.0E-11
Sequence
Target Sequence 
>C. ELEGANS:186056_S_AT
gatatcattctggaggtcctcgacaaccttatccacgaaagacaactggtcaactgatgg
cttctgagtattcactggaatctatggttgcagctgaatttgctccgaatttgagtacga
aagagttgggaactcagatggccgccgcaatgggagaaaaggatccggatacggtgaaaa
agattacaaacgcgattggctcggaactatcgattaaattattcaatgagacaaaagaaa
tcgagcaaaatggtggaatgaagactgcggatggaagtcgtcgtcggactcccggtggag
tattcattacacttttcaagatggattcatcggttagccgagagattaaggatggcattt
ttggagatatgaggaatgctgaaaaagagagaactaagaagaagaagggagagaaacagt
ttacgagacagcttgaagatatgaagaaaacgatggaactcgccgctcaagccgaaaccg
atttggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATATCATTCTGGAGGTCCTCGACA 298 425 485 Antisense
TCCTCGACAACCTTATCCACGAAAG 457 593 501 Antisense
GACAACTGGTCAACTGATGGCTTCT 640 365 525 Antisense
GATGGCTTCTGAGTATTCACTGGAA 382 407 540 Antisense
GTTGCAGCTGAATTTGCTCCGAATT 554 439 571 Antisense
CTCAGATGGCCGCCGCAATGGGAGA 360 227 617 Antisense
CAAACGCGATTGGCTCGGAACTATC 605 189 671 Antisense
ACTGCGGATGGAAGTCGTCGTCGGA 187 97 748 Antisense
TCGTCGGACTCCCGGTGGAGTATTC 98 601 765 Antisense
AGAAAACGATGGAACTCGCCGCTCA 270 73 929 Antisense
CCGCTCAAGCCGAAACCGATTTGGC 378 261 947 Antisense
Sequence Source Affymetrix Proprietary Database